NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus

Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Samuel Santos Pereira, Robert Andreata-Santos, Lennon Ramos Pereira, Camila Pereira Soares, Alvina Clara Félix, Patrícia de Mello Jungmann Cardoso de Andrade, Edison Luís Durigon, Camila Malta Romano, Luís Carlos de Souza Ferreira
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Dengue virus Serological diagnosis NS1 ELISA Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/660dc5acfb314465b99606cb9fe8d15a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:660dc5acfb314465b99606cb9fe8d15a
record_format	dspace
spelling	oai:doaj.org-article:660dc5acfb314465b99606cb9fe8d15a2021-11-30T04:14:09ZNS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus1201-971210.1016/j.ijid.2021.09.009https://doaj.org/article/660dc5acfb314465b99606cb9fe8d15a2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221007190https://doaj.org/toc/1201-9712Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The ΔNS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the ∆NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The ∆NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The ∆NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission.Samuel Santos PereiraRobert Andreata-SantosLennon Ramos PereiraCamila Pereira SoaresAlvina Clara FélixPatrícia de Mello Jungmann Cardoso de AndradeEdison Luís DurigonCamila Malta RomanoLuís Carlos de Souza FerreiraElsevierarticleDengue virusSerological diagnosisNS1ELISAInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 112, Iss , Pp 202-204 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dengue virus Serological diagnosis NS1 ELISA Infectious and parasitic diseases RC109-216
spellingShingle	Dengue virus Serological diagnosis NS1 ELISA Infectious and parasitic diseases RC109-216 Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
description	Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (ΔNS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The ΔNS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the ∆NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The ∆NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The ∆NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission.
format	article
author	Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira
author_facet	Samuel Santos Pereira Robert Andreata-Santos Lennon Ramos Pereira Camila Pereira Soares Alvina Clara Félix Patrícia de Mello Jungmann Cardoso de Andrade Edison Luís Durigon Camila Malta Romano Luís Carlos de Souza Ferreira
author_sort	Samuel Santos Pereira
title	NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
title_short	NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
title_full	NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
title_fullStr	NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
title_full_unstemmed	NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
title_sort	ns1-based elisa test efficiently detects dengue infections without cross-reactivity with zika virus
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/660dc5acfb314465b99606cb9fe8d15a
work_keys_str_mv	AT samuelsantospereira ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT robertandreatasantos ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT lennonramospereira ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT camilapereirasoares ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT alvinaclarafelix ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT patriciademellojungmanncardosodeandrade ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT edisonluisdurigon ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT camilamaltaromano ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus AT luiscarlosdesouzaferreira ns1basedelisatestefficientlydetectsdengueinfectionswithoutcrossreactivitywithzikavirus
_version_	1718406805887909888

NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus

Ejemplares similares