Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections

Nosocomial infections caused by antibiotic-resistant bacteria are constantly growing healthcare threats, as they are the reason for the increased mortality, morbidity, and considerable financial burden due to the poor infection outcomes. Indwelling medical devices, such as urinary catheters, are fre...

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Autores principales:	Aleksandra Ivanova, Kristina Ivanova, Tzanko Tzanov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Polymyxin B poly(sulfobetaine) methacrylate sonochemistry nanohybrids antibacterial activity biofilm inhibition Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/661f6d6b6da64952a12a86438765ca01
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spelling	oai:doaj.org-article:661f6d6b6da64952a12a86438765ca012021-11-25T18:32:49ZSimultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections10.3390/nano111131432079-4991https://doaj.org/article/661f6d6b6da64952a12a86438765ca012021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3143https://doaj.org/toc/2079-4991Nosocomial infections caused by antibiotic-resistant bacteria are constantly growing healthcare threats, as they are the reason for the increased mortality, morbidity, and considerable financial burden due to the poor infection outcomes. Indwelling medical devices, such as urinary catheters, are frequently colonized by bacteria in the form of biofilms that cause dysfunction of the device and severe chronic infections. The current treatment strategies of such device-associated infections are impaired by the resistant pathogens but also by a risk of prompting the appearance of new antibiotic-resistant bacterial mechanisms. Herein, the one-step sonochemical synthesis of hybrid poly(sulfobetaine) methacrylate/Polymyxin B nanoparticles (pSBMA@PM NPs) coating was employed to engineer novel nanoenabled silicone catheters with improved antifouling, antibacterial, and antibiofilm efficiencies. The synergistic mode of action of nanohybridized zwitterionic polymer and antimicrobial peptide led to complete inhibition of the nonspecific protein adsorption and up to 97% reduction in <i>Pseudomonas aeruginosa</i> biofilm formation, in comparison with the pristine silicone. Additionally, the bactericidal activity in the hybrid coating reduced the free-floating and surface-attached bacterial growth by 8 logs, minimizing the probability for further <i>P. aeruginosa</i> spreading and host invasion. This coating was stable for up to 7 days under conditions simulating the real scenario of catheter usage and inhibited by 80% <i>P. aeruginosa</i> biofilms. For the same time of use, the pSBMA@PM NPs coating did not affect the metabolic activity and morphology of mammalian cells, demonstrating their capacity to control antibiotic-resistant biofilm-associated bacterial infections.Aleksandra IvanovaKristina IvanovaTzanko TzanovMDPI AGarticlePolymyxin Bpoly(sulfobetaine) methacrylatesonochemistrynanohybridsantibacterial activitybiofilm inhibitionChemistryQD1-999ENNanomaterials, Vol 11, Iss 3143, p 3143 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polymyxin B poly(sulfobetaine) methacrylate sonochemistry nanohybrids antibacterial activity biofilm inhibition Chemistry QD1-999
spellingShingle	Polymyxin B poly(sulfobetaine) methacrylate sonochemistry nanohybrids antibacterial activity biofilm inhibition Chemistry QD1-999 Aleksandra Ivanova Kristina Ivanova Tzanko Tzanov Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
description	Nosocomial infections caused by antibiotic-resistant bacteria are constantly growing healthcare threats, as they are the reason for the increased mortality, morbidity, and considerable financial burden due to the poor infection outcomes. Indwelling medical devices, such as urinary catheters, are frequently colonized by bacteria in the form of biofilms that cause dysfunction of the device and severe chronic infections. The current treatment strategies of such device-associated infections are impaired by the resistant pathogens but also by a risk of prompting the appearance of new antibiotic-resistant bacterial mechanisms. Herein, the one-step sonochemical synthesis of hybrid poly(sulfobetaine) methacrylate/Polymyxin B nanoparticles (pSBMA@PM NPs) coating was employed to engineer novel nanoenabled silicone catheters with improved antifouling, antibacterial, and antibiofilm efficiencies. The synergistic mode of action of nanohybridized zwitterionic polymer and antimicrobial peptide led to complete inhibition of the nonspecific protein adsorption and up to 97% reduction in <i>Pseudomonas aeruginosa</i> biofilm formation, in comparison with the pristine silicone. Additionally, the bactericidal activity in the hybrid coating reduced the free-floating and surface-attached bacterial growth by 8 logs, minimizing the probability for further <i>P. aeruginosa</i> spreading and host invasion. This coating was stable for up to 7 days under conditions simulating the real scenario of catheter usage and inhibited by 80% <i>P. aeruginosa</i> biofilms. For the same time of use, the pSBMA@PM NPs coating did not affect the metabolic activity and morphology of mammalian cells, demonstrating their capacity to control antibiotic-resistant biofilm-associated bacterial infections.
format	article
author	Aleksandra Ivanova Kristina Ivanova Tzanko Tzanov
author_facet	Aleksandra Ivanova Kristina Ivanova Tzanko Tzanov
author_sort	Aleksandra Ivanova
title	Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
title_short	Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
title_full	Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
title_fullStr	Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
title_full_unstemmed	Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections
title_sort	simultaneous ultrasound-assisted hybrid polyzwitterion/antimicrobial peptide nanoparticles synthesis and deposition on silicone urinary catheters for prevention of biofilm-associated infections
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/661f6d6b6da64952a12a86438765ca01
work_keys_str_mv	AT aleksandraivanova simultaneousultrasoundassistedhybridpolyzwitterionantimicrobialpeptidenanoparticlessynthesisanddepositiononsiliconeurinarycathetersforpreventionofbiofilmassociatedinfections AT kristinaivanova simultaneousultrasoundassistedhybridpolyzwitterionantimicrobialpeptidenanoparticlessynthesisanddepositiononsiliconeurinarycathetersforpreventionofbiofilmassociatedinfections AT tzankotzanov simultaneousultrasoundassistedhybridpolyzwitterionantimicrobialpeptidenanoparticlessynthesisanddepositiononsiliconeurinarycathetersforpreventionofbiofilmassociatedinfections
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Simultaneous Ultrasound-Assisted Hybrid Polyzwitterion/Antimicrobial Peptide Nanoparticles Synthesis and Deposition on Silicone Urinary Catheters for Prevention of Biofilm-Associated Infections

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