Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis

Tsutomu Sakai1, Kenichi Kohzaki1, Akira Watanabe1, Hiroshi Tsuneoka1, Mitsunobu Shimadzu21Department of Ophthalmology, Jikei University School of Medicine, 2Mitsubishi Chemical Medience Corporation, Tokyo, JapanBackground: To examine the utility of DNA microarray analysis for identifying causative m...

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Autores principales:	Sakai T, Kohzaki K, Watanabe A, Tsuneoka H, Shimadzu M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/6621c30623ee4175a1ad86060586ab2e
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spelling	oai:doaj.org-article:6621c30623ee4175a1ad86060586ab2e2021-12-02T02:45:18ZUse of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis1177-54671177-5483https://doaj.org/article/6621c30623ee4175a1ad86060586ab2e2012-02-01T00:00:00Zhttp://www.dovepress.com/use-of-dna-microarray-analysis-in-diagnosis-of-bacterial-and-fungal-en-a9379https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Tsutomu Sakai1, Kenichi Kohzaki1, Akira Watanabe1, Hiroshi Tsuneoka1, Mitsunobu Shimadzu21Department of Ophthalmology, Jikei University School of Medicine, 2Mitsubishi Chemical Medience Corporation, Tokyo, JapanBackground: To examine the utility of DNA microarray analysis for identifying causative microorganisms in endophthalmitis.Methods: Thirteen samples of vitreous fluid (VF) were obtained from 13 patients during vitrectomy. Vitreous fluids from three patients with suspected endophthalmitis and ten controls without infection were subjected to testing for the presence of bacteria and fungi in culture tests, polymerase chain reaction (PCR) analysis, and DNA microarray analysis.Results: No control sample was positive for bacteria or fungi in the culture test, PCR, or microarray analysis. Specimens from two patients (Cases 1 and 2) with suspected endophthalmitis were positive for bacteria in PCR, and a specimen from one patient (Case 3) was positive for fungi in PCR. Klebsiella pneumonia (Case 1), Streptococcus agalactiae (Case 2), and Candida parapsilosis (Case 3) in the PCR-positive specimens were identified by DNA microarray analysis within 24 hours. Culture results were also positive for K. pneumonia in Case 1, S. agalactiae in Case 2, and C. parapsilosis in Case 3, but required 3 to 4 days to obtain.Conclusions: Microarray analysis is complementary to routine cultures for identifying causative microorganisms and is likely to be a useful tool in patients with suspected endophthalmitis who require rapid diagnosis and early antibiotic treatment.Keywords: DNA microarray, endophthalmitis, microorganism, vitreous fluidSakai TKohzaki KWatanabe ATsuneoka HShimadzu MDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 321-326 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Sakai T Kohzaki K Watanabe A Tsuneoka H Shimadzu M Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
description	Tsutomu Sakai1, Kenichi Kohzaki1, Akira Watanabe1, Hiroshi Tsuneoka1, Mitsunobu Shimadzu21Department of Ophthalmology, Jikei University School of Medicine, 2Mitsubishi Chemical Medience Corporation, Tokyo, JapanBackground: To examine the utility of DNA microarray analysis for identifying causative microorganisms in endophthalmitis.Methods: Thirteen samples of vitreous fluid (VF) were obtained from 13 patients during vitrectomy. Vitreous fluids from three patients with suspected endophthalmitis and ten controls without infection were subjected to testing for the presence of bacteria and fungi in culture tests, polymerase chain reaction (PCR) analysis, and DNA microarray analysis.Results: No control sample was positive for bacteria or fungi in the culture test, PCR, or microarray analysis. Specimens from two patients (Cases 1 and 2) with suspected endophthalmitis were positive for bacteria in PCR, and a specimen from one patient (Case 3) was positive for fungi in PCR. Klebsiella pneumonia (Case 1), Streptococcus agalactiae (Case 2), and Candida parapsilosis (Case 3) in the PCR-positive specimens were identified by DNA microarray analysis within 24 hours. Culture results were also positive for K. pneumonia in Case 1, S. agalactiae in Case 2, and C. parapsilosis in Case 3, but required 3 to 4 days to obtain.Conclusions: Microarray analysis is complementary to routine cultures for identifying causative microorganisms and is likely to be a useful tool in patients with suspected endophthalmitis who require rapid diagnosis and early antibiotic treatment.Keywords: DNA microarray, endophthalmitis, microorganism, vitreous fluid
format	article
author	Sakai T Kohzaki K Watanabe A Tsuneoka H Shimadzu M
author_facet	Sakai T Kohzaki K Watanabe A Tsuneoka H Shimadzu M
author_sort	Sakai T
title	Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
title_short	Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
title_full	Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
title_fullStr	Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
title_full_unstemmed	Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
title_sort	use of dna microarray analysis in diagnosis of bacterial and fungal endophthalmitis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/6621c30623ee4175a1ad86060586ab2e
work_keys_str_mv	AT sakait useofdnamicroarrayanalysisindiagnosisofbacterialandfungalendophthalmitis AT kohzakik useofdnamicroarrayanalysisindiagnosisofbacterialandfungalendophthalmitis AT watanabea useofdnamicroarrayanalysisindiagnosisofbacterialandfungalendophthalmitis AT tsuneokah useofdnamicroarrayanalysisindiagnosisofbacterialandfungalendophthalmitis AT shimadzum useofdnamicroarrayanalysisindiagnosisofbacterialandfungalendophthalmitis
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Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis

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