Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.

A filamentous fungus, Magnaporthe oryzae, is a causal agent of rice blast disease, which is one of the most serious diseases affecting cultivated rice, Oryza sativa. However, the molecular mechanisms underlying both rice defense and fungal attack are not yet fully understood. Extensive past studies...

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Autores principales:	Yoshihiro Kawahara, Youko Oono, Hiroyuki Kanamori, Takashi Matsumoto, Takeshi Itoh, Eiichi Minami
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6628325e08dd4368a39e60d9cde12ce5
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spelling	oai:doaj.org-article:6628325e08dd4368a39e60d9cde12ce52021-11-18T08:09:52ZSimultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.1932-620310.1371/journal.pone.0049423https://doaj.org/article/6628325e08dd4368a39e60d9cde12ce52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139845/?tool=EBIhttps://doaj.org/toc/1932-6203A filamentous fungus, Magnaporthe oryzae, is a causal agent of rice blast disease, which is one of the most serious diseases affecting cultivated rice, Oryza sativa. However, the molecular mechanisms underlying both rice defense and fungal attack are not yet fully understood. Extensive past studies have characterized many infection-responsive genes in the pathogen and host plant, separately. To understand the plant-pathogen interaction comprehensively, it is valuable to monitor the gene expression profiles of both interacting organisms simultaneously in the same infected plant tissue. Although the host-pathogen interaction during the initial infection stage is important for the establishment of infection, the detection of fungal gene expression in infected leaves at the stage has been difficult because very few numbers of fungal cells are present. Using the emerging RNA-Seq technique, which has a wide dynamic range for expression analyses, we analyzed the mixed transcriptome of rice and blast fungus in infected leaves at 24 hours post-inoculation, which is the point when the primary infection hyphae penetrate leaf epidermal cells. We demonstrated that our method detected the gene expression of both the host plant and pathogen simultaneously in the same infected leaf blades in natural infection conditions without any artificial treatments. The upregulation of 240 fungal transcripts encoding putative secreted proteins was observed, suggesting that these candidates of fungal effector genes may play important roles in initial infection processes. The upregulation of transcripts encoding glycosyl hydrolases, cutinases and LysM domain-containing proteins were observed in the blast fungus, whereas pathogenesis-related and phytoalexin biosynthetic genes were upregulated in rice. Furthermore, more drastic changes in expression were observed in the incompatible interactions compared with the compatible ones in both rice and blast fungus at this stage. Our mixed transcriptome analysis is useful for the simultaneous elucidation of the tactics of host plant defense and pathogen attack.Yoshihiro KawaharaYouko OonoHiroyuki KanamoriTakashi MatsumotoTakeshi ItohEiichi MinamiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49423 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshihiro Kawahara Youko Oono Hiroyuki Kanamori Takashi Matsumoto Takeshi Itoh Eiichi Minami Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
description	A filamentous fungus, Magnaporthe oryzae, is a causal agent of rice blast disease, which is one of the most serious diseases affecting cultivated rice, Oryza sativa. However, the molecular mechanisms underlying both rice defense and fungal attack are not yet fully understood. Extensive past studies have characterized many infection-responsive genes in the pathogen and host plant, separately. To understand the plant-pathogen interaction comprehensively, it is valuable to monitor the gene expression profiles of both interacting organisms simultaneously in the same infected plant tissue. Although the host-pathogen interaction during the initial infection stage is important for the establishment of infection, the detection of fungal gene expression in infected leaves at the stage has been difficult because very few numbers of fungal cells are present. Using the emerging RNA-Seq technique, which has a wide dynamic range for expression analyses, we analyzed the mixed transcriptome of rice and blast fungus in infected leaves at 24 hours post-inoculation, which is the point when the primary infection hyphae penetrate leaf epidermal cells. We demonstrated that our method detected the gene expression of both the host plant and pathogen simultaneously in the same infected leaf blades in natural infection conditions without any artificial treatments. The upregulation of 240 fungal transcripts encoding putative secreted proteins was observed, suggesting that these candidates of fungal effector genes may play important roles in initial infection processes. The upregulation of transcripts encoding glycosyl hydrolases, cutinases and LysM domain-containing proteins were observed in the blast fungus, whereas pathogenesis-related and phytoalexin biosynthetic genes were upregulated in rice. Furthermore, more drastic changes in expression were observed in the incompatible interactions compared with the compatible ones in both rice and blast fungus at this stage. Our mixed transcriptome analysis is useful for the simultaneous elucidation of the tactics of host plant defense and pathogen attack.
format	article
author	Yoshihiro Kawahara Youko Oono Hiroyuki Kanamori Takashi Matsumoto Takeshi Itoh Eiichi Minami
author_facet	Yoshihiro Kawahara Youko Oono Hiroyuki Kanamori Takashi Matsumoto Takeshi Itoh Eiichi Minami
author_sort	Yoshihiro Kawahara
title	Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
title_short	Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
title_full	Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
title_fullStr	Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
title_full_unstemmed	Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
title_sort	simultaneous rna-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6628325e08dd4368a39e60d9cde12ce5
work_keys_str_mv	AT yoshihirokawahara simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction AT youkooono simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction AT hiroyukikanamori simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction AT takashimatsumoto simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction AT takeshiitoh simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction AT eiichiminami simultaneousrnaseqanalysisofamixedtranscriptomeofriceandblastfungusinteraction
_version_	1718422110659936256

Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.

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