Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma

Lingling Ou,1 Ting Sun,1 Minyi Liu,1 Ye Zhang,1 Zhiying Zhou,1 Xiaozhen Zhan,1 Lihong Lu,1 Qingtong Zhao,1 Renfa Lai,1 Longquan Shao2 1The First Affiliated Hospital of Jinan University, Department of Stomatology, Guangzhou 510632, People’s Republic of China; 2Stomatological Hospital of Sou...

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Autores principales:	Ou L, Sun T, Liu M, Zhang Y, Zhou Z, Zhan X, Lu L, Zhao Q, Lai R, Shao L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	oral squamous cell carcinoma go-pei mir-214 inhibitor gene therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/662a50544a464d6183f00c9ce2ad74f2
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spelling	oai:doaj.org-article:662a50544a464d6183f00c9ce2ad74f22021-12-02T08:48:07ZEfficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma1178-2013https://doaj.org/article/662a50544a464d6183f00c9ce2ad74f22020-03-01T00:00:00Zhttps://www.dovepress.com/efficient-mirna-inhibitor-delivery-with-graphene-oxide-polyethylenimin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lingling Ou,1 Ting Sun,1 Minyi Liu,1 Ye Zhang,1 Zhiying Zhou,1 Xiaozhen Zhan,1 Lihong Lu,1 Qingtong Zhao,1 Renfa Lai,1 Longquan Shao2 1The First Affiliated Hospital of Jinan University, Department of Stomatology, Guangzhou 510632, People’s Republic of China; 2Stomatological Hospital of Southern Medical University, Department of Prosthodontics, Guangzhou 510260, People’s Republic of ChinaCorrespondence: Renfa LaiThe First Affiliated Hospital of Jinan University, No.613 West Huangpu Avenue, Guangzhou 510632, People’s Republic of ChinaEmail Prof.Dr.Lai@163.comLongquan ShaoStomatological Hospital of Southern Medical University, No. 366 South Jiangnan Avenue, Guangzhou 510260, People’s Republic of ChinaEmail shaolongquan@smu.edu.cnBackground: MicroRNAs (miRNAs) are widely believed to be promising targets for oral squamous cell carcinoma (OSCC) gene therapy. miR-214 has been identified as a promoter of OSCC aggression and metastasis.Methods: Graphene oxide-polyethylenimine (GO-PEI) complexes were prepared and loaded with a miRNA inhibitor at different N/P ratios. The transfection efficiency of GO-PEI-inhibitor was tested in Cal27 and SCC9 cells. Moreover, the tumor inhibition ability of GO-PEI-inhibitor was measured in an OSCC xenograft mouse model by intratumoral injection.Results: Here, we show that a GO-PEI complex efficiently delivers a miR-214 inhibitor into OSCC cells and controls the intracellular release of the miR-214 inhibitor. These results indicate that the GO-PEI-miR-214 inhibitor complex efficiently inhibited cellular miR-214, resulting in a decrease in OSCC cell invasion and migration and an increase in cell apoptosis by targeting PTEN and p53. In the xenograft mouse model, the GO-PEI-miR-214 inhibitor complex significantly prevented tumor volume growth.Conclusion: This study indicates that functionalized GO-PEI with low toxicity has promising potential for miRNA delivery for the treatment of OSCC.Keywords: oral squamous cell carcinoma, GO-PEI, miR-214 inhibitor, gene therapyOu LSun TLiu MZhang YZhou ZZhan XLu LZhao QLai RShao LDove Medical Pressarticleoral squamous cell carcinomago-peimir-214 inhibitorgene therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1569-1583 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral squamous cell carcinoma go-pei mir-214 inhibitor gene therapy Medicine (General) R5-920
spellingShingle	oral squamous cell carcinoma go-pei mir-214 inhibitor gene therapy Medicine (General) R5-920 Ou L Sun T Liu M Zhang Y Zhou Z Zhan X Lu L Zhao Q Lai R Shao L Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
description	Lingling Ou,1 Ting Sun,1 Minyi Liu,1 Ye Zhang,1 Zhiying Zhou,1 Xiaozhen Zhan,1 Lihong Lu,1 Qingtong Zhao,1 Renfa Lai,1 Longquan Shao2 1The First Affiliated Hospital of Jinan University, Department of Stomatology, Guangzhou 510632, People’s Republic of China; 2Stomatological Hospital of Southern Medical University, Department of Prosthodontics, Guangzhou 510260, People’s Republic of ChinaCorrespondence: Renfa LaiThe First Affiliated Hospital of Jinan University, No.613 West Huangpu Avenue, Guangzhou 510632, People’s Republic of ChinaEmail Prof.Dr.Lai@163.comLongquan ShaoStomatological Hospital of Southern Medical University, No. 366 South Jiangnan Avenue, Guangzhou 510260, People’s Republic of ChinaEmail shaolongquan@smu.edu.cnBackground: MicroRNAs (miRNAs) are widely believed to be promising targets for oral squamous cell carcinoma (OSCC) gene therapy. miR-214 has been identified as a promoter of OSCC aggression and metastasis.Methods: Graphene oxide-polyethylenimine (GO-PEI) complexes were prepared and loaded with a miRNA inhibitor at different N/P ratios. The transfection efficiency of GO-PEI-inhibitor was tested in Cal27 and SCC9 cells. Moreover, the tumor inhibition ability of GO-PEI-inhibitor was measured in an OSCC xenograft mouse model by intratumoral injection.Results: Here, we show that a GO-PEI complex efficiently delivers a miR-214 inhibitor into OSCC cells and controls the intracellular release of the miR-214 inhibitor. These results indicate that the GO-PEI-miR-214 inhibitor complex efficiently inhibited cellular miR-214, resulting in a decrease in OSCC cell invasion and migration and an increase in cell apoptosis by targeting PTEN and p53. In the xenograft mouse model, the GO-PEI-miR-214 inhibitor complex significantly prevented tumor volume growth.Conclusion: This study indicates that functionalized GO-PEI with low toxicity has promising potential for miRNA delivery for the treatment of OSCC.Keywords: oral squamous cell carcinoma, GO-PEI, miR-214 inhibitor, gene therapy
format	article
author	Ou L Sun T Liu M Zhang Y Zhou Z Zhan X Lu L Zhao Q Lai R Shao L
author_facet	Ou L Sun T Liu M Zhang Y Zhou Z Zhan X Lu L Zhao Q Lai R Shao L
author_sort	Ou L
title	Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
title_short	Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
title_full	Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
title_fullStr	Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
title_full_unstemmed	Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
title_sort	efficient mirna inhibitor delivery with graphene oxide-polyethylenimine to inhibit oral squamous cell carcinoma
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/662a50544a464d6183f00c9ce2ad74f2
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Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma

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