Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity

Background. ERK1 and ERK2 (extracellular signal-regulated kinase) are related protein serine / threonine kinases that are involved in the regulation of the cell cycle, cell migration and survival, differentiation, metabolism, and proliferation. Evaluation of this cascade is promising to determine th...

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Autores principales:	I. A. Shurygina, M. G. Shurygin, L. V. Rodionova, E. E. Chepurnykh, N. I. Ayushinova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	adhesive process mapk erk1 erk2 Science Q
Acceso en línea:	https://doaj.org/article/663ad07bb2c84181bffb9a419eb54b06
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spelling	oai:doaj.org-article:663ad07bb2c84181bffb9a419eb54b062021-11-23T06:14:45ZInvolvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity2541-94202587-959610.29413/ABS.2020-5.6.33https://doaj.org/article/663ad07bb2c84181bffb9a419eb54b062021-01-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2524https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. ERK1 and ERK2 (extracellular signal-regulated kinase) are related protein serine / threonine kinases that are involved in the regulation of the cell cycle, cell migration and survival, differentiation, metabolism, and proliferation. Evaluation of this cascade is promising to determine the possibility of influencing pathological conditions through the regulation of ERK1/2.Aim of the study. To assess the involvement of the ERK MAPK cascade in the development of adhesions in the abdominal cavity.Methods. The experiments were carried out on male Wistar rats. The adhesion process in the abdominal cavity was simulated (n = 40). The research time is from 2 hours to 30 days. Tissue samples from 5 intact animals were examined as a control. Histological sections were prepared and stained histochemically for ERK1. The expression of ERK1 and ERK2 was assessed by PCR.Results. Using the PCR method, the involvement of both the ERK1 and ERK2 cascades in the process was established, and, judging by the change in gene expression, the latter is more actively involved in the process. Histochemical study confirmed the role of ERK cascades in the management of repair in serous membrane injury. Histochemically revealed an increase in ERK1 expression with two peaks of activity – at 1 and 30 days.Conclusion. Our data establish multichannel control of the reparative process in the area of damage to the serous membrane with the involvement of both the p38 and ERK MAPK cascades, which is important to consider when developing methods of influencing the wound healing process in the abdominal cavity.I. A. ShuryginaM. G. ShuryginL. V. RodionovaE. E. ChepurnykhN. I. AyushinovaScientific Сentre for Family Health and Human Reproduction Problemsarticleadhesive processmapkerk1erk2ScienceQRUActa Biomedica Scientifica, Vol 5, Iss 6, Pp 254-258 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	adhesive process mapk erk1 erk2 Science Q
spellingShingle	adhesive process mapk erk1 erk2 Science Q I. A. Shurygina M. G. Shurygin L. V. Rodionova E. E. Chepurnykh N. I. Ayushinova Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
description	Background. ERK1 and ERK2 (extracellular signal-regulated kinase) are related protein serine / threonine kinases that are involved in the regulation of the cell cycle, cell migration and survival, differentiation, metabolism, and proliferation. Evaluation of this cascade is promising to determine the possibility of influencing pathological conditions through the regulation of ERK1/2.Aim of the study. To assess the involvement of the ERK MAPK cascade in the development of adhesions in the abdominal cavity.Methods. The experiments were carried out on male Wistar rats. The adhesion process in the abdominal cavity was simulated (n = 40). The research time is from 2 hours to 30 days. Tissue samples from 5 intact animals were examined as a control. Histological sections were prepared and stained histochemically for ERK1. The expression of ERK1 and ERK2 was assessed by PCR.Results. Using the PCR method, the involvement of both the ERK1 and ERK2 cascades in the process was established, and, judging by the change in gene expression, the latter is more actively involved in the process. Histochemical study confirmed the role of ERK cascades in the management of repair in serous membrane injury. Histochemically revealed an increase in ERK1 expression with two peaks of activity – at 1 and 30 days.Conclusion. Our data establish multichannel control of the reparative process in the area of damage to the serous membrane with the involvement of both the p38 and ERK MAPK cascades, which is important to consider when developing methods of influencing the wound healing process in the abdominal cavity.
format	article
author	I. A. Shurygina M. G. Shurygin L. V. Rodionova E. E. Chepurnykh N. I. Ayushinova
author_facet	I. A. Shurygina M. G. Shurygin L. V. Rodionova E. E. Chepurnykh N. I. Ayushinova
author_sort	I. A. Shurygina
title	Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
title_short	Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
title_full	Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
title_fullStr	Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
title_full_unstemmed	Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity
title_sort	involvement of the erk mapk cascade in the formation of adhesions in the abdominal cavity
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/663ad07bb2c84181bffb9a419eb54b06
work_keys_str_mv	AT iashurygina involvementoftheerkmapkcascadeintheformationofadhesionsintheabdominalcavity AT mgshurygin involvementoftheerkmapkcascadeintheformationofadhesionsintheabdominalcavity AT lvrodionova involvementoftheerkmapkcascadeintheformationofadhesionsintheabdominalcavity AT eechepurnykh involvementoftheerkmapkcascadeintheformationofadhesionsintheabdominalcavity AT niayushinova involvementoftheerkmapkcascadeintheformationofadhesionsintheabdominalcavity
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Involvement of the ERK MAPK Cascade in the Formation of Adhesions in the Abdominal Cavity

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