Ectoine can enhance structural changes in DNA in vitro

Abstract Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulat...

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Autores principales:	S. Meyer, M.-A. Schröter, M. B. Hahn, T. Solomun, H. Sturm, H. J. Kunte
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/663b00e8106040f6bbb3608387caae94
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spelling	oai:doaj.org-article:663b00e8106040f6bbb3608387caae942021-12-02T11:40:41ZEctoine can enhance structural changes in DNA in vitro10.1038/s41598-017-07441-z2045-2322https://doaj.org/article/663b00e8106040f6bbb3608387caae942017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07441-zhttps://doaj.org/toc/2045-2322Abstract Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulated that ectoine-like molecules bind to negatively charged spheres that mimic DNA surfaces. We investigated the effect of ectoine on DNA and whether ectoine is able to protect DNA from damages caused by ultraviolet radiation (UV-A). In order to determine different isoforms of DNA, agarose gel electrophoresis and atomic force microscopy experiments were carried out with plasmid pUC19 DNA. Our quantitative results revealed that a prolonged incubation of DNA with ectoine leads to an increase in transitions from supercoiled (undamaged) to open circular (single-strand break) conformation at pH 6.6. The effect is pH dependent and no significant changes were observed at physiological pH of 7.5. After UV-A irradiation in ectoine solution, changes in DNA conformation were even more pronounced and this effect was pH dependent. We hypothesize that ectoine is attracted to the negatively charge surface of DNA at lower pH and therefore fails to act as a stabilizing agent for DNA in our in vitro experiments.S. MeyerM.-A. SchröterM. B. HahnT. SolomunH. SturmH. J. KunteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Meyer M.-A. Schröter M. B. Hahn T. Solomun H. Sturm H. J. Kunte Ectoine can enhance structural changes in DNA in vitro
description	Abstract Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulated that ectoine-like molecules bind to negatively charged spheres that mimic DNA surfaces. We investigated the effect of ectoine on DNA and whether ectoine is able to protect DNA from damages caused by ultraviolet radiation (UV-A). In order to determine different isoforms of DNA, agarose gel electrophoresis and atomic force microscopy experiments were carried out with plasmid pUC19 DNA. Our quantitative results revealed that a prolonged incubation of DNA with ectoine leads to an increase in transitions from supercoiled (undamaged) to open circular (single-strand break) conformation at pH 6.6. The effect is pH dependent and no significant changes were observed at physiological pH of 7.5. After UV-A irradiation in ectoine solution, changes in DNA conformation were even more pronounced and this effect was pH dependent. We hypothesize that ectoine is attracted to the negatively charge surface of DNA at lower pH and therefore fails to act as a stabilizing agent for DNA in our in vitro experiments.
format	article
author	S. Meyer M.-A. Schröter M. B. Hahn T. Solomun H. Sturm H. J. Kunte
author_facet	S. Meyer M.-A. Schröter M. B. Hahn T. Solomun H. Sturm H. J. Kunte
author_sort	S. Meyer
title	Ectoine can enhance structural changes in DNA in vitro
title_short	Ectoine can enhance structural changes in DNA in vitro
title_full	Ectoine can enhance structural changes in DNA in vitro
title_fullStr	Ectoine can enhance structural changes in DNA in vitro
title_full_unstemmed	Ectoine can enhance structural changes in DNA in vitro
title_sort	ectoine can enhance structural changes in dna in vitro
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/663b00e8106040f6bbb3608387caae94
work_keys_str_mv	AT smeyer ectoinecanenhancestructuralchangesindnainvitro AT maschroter ectoinecanenhancestructuralchangesindnainvitro AT mbhahn ectoinecanenhancestructuralchangesindnainvitro AT tsolomun ectoinecanenhancestructuralchangesindnainvitro AT hsturm ectoinecanenhancestructuralchangesindnainvitro AT hjkunte ectoinecanenhancestructuralchangesindnainvitro
_version_	1718395585786019840

Ectoine can enhance structural changes in DNA in vitro

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