Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.

We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undiffere...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gnanaratnam Giritharan, Dusko Ilic, Matthew Gormley, Ana Krtolica
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/663d2cddc2f247bbab0124f14323e5ee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:663d2cddc2f247bbab0124f14323e5ee
record_format	dspace
spelling	oai:doaj.org-article:663d2cddc2f247bbab0124f14323e5ee2021-11-18T07:35:56ZHuman embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.1932-620310.1371/journal.pone.0026570https://doaj.org/article/663d2cddc2f247bbab0124f14323e5ee2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039509/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undifferentiated proliferation, genomic stability, expression of pluripotency markers and the ability to differentiate into the cells of all three germ layers both in vitro and in vivo. To examine whether hESC lines derived from two developmental stages of the embryo differ in gene expression, we have subjected three blastomere-derived hESC lines and two ICM-derived hESC lines grown under identical culture conditions to transcriptome analysis using gene expression arrays. Unlike previously reported comparisons of hESC lines which demonstrated, apart from core hESC-associated pluripotency signature, significant variations in gene expression profiles of different lines, our data show that hESC lines derived and grown under well-controlled defined culture conditions adopt nearly identical gene expression profiles. Moreover, blastomere-derived and ICM-derived hESC exhibited very similar transcriptional profiles independent of the developmental stage of the embryo from which they originated. Furthermore, this profile was evident in very early passages of the cells and did not appear to be affected by extensive passaging. These results suggest that during derivation process cells which give rise to hESC acquire virtually identical stable phenotype and are not affected by the developmental stage of the starting cell population.Gnanaratnam GiritharanDusko IlicMatthew GormleyAna KrtolicaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26570 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gnanaratnam Giritharan Dusko Ilic Matthew Gormley Ana Krtolica Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
description	We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undifferentiated proliferation, genomic stability, expression of pluripotency markers and the ability to differentiate into the cells of all three germ layers both in vitro and in vivo. To examine whether hESC lines derived from two developmental stages of the embryo differ in gene expression, we have subjected three blastomere-derived hESC lines and two ICM-derived hESC lines grown under identical culture conditions to transcriptome analysis using gene expression arrays. Unlike previously reported comparisons of hESC lines which demonstrated, apart from core hESC-associated pluripotency signature, significant variations in gene expression profiles of different lines, our data show that hESC lines derived and grown under well-controlled defined culture conditions adopt nearly identical gene expression profiles. Moreover, blastomere-derived and ICM-derived hESC exhibited very similar transcriptional profiles independent of the developmental stage of the embryo from which they originated. Furthermore, this profile was evident in very early passages of the cells and did not appear to be affected by extensive passaging. These results suggest that during derivation process cells which give rise to hESC acquire virtually identical stable phenotype and are not affected by the developmental stage of the starting cell population.
format	article
author	Gnanaratnam Giritharan Dusko Ilic Matthew Gormley Ana Krtolica
author_facet	Gnanaratnam Giritharan Dusko Ilic Matthew Gormley Ana Krtolica
author_sort	Gnanaratnam Giritharan
title	Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
title_short	Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
title_full	Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
title_fullStr	Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
title_full_unstemmed	Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
title_sort	human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/663d2cddc2f247bbab0124f14323e5ee
work_keys_str_mv	AT gnanaratnamgiritharan humanembryonicstemcellsderivedfromembryosatdifferentstagesofdevelopmentsharesimilartranscriptionprofiles AT duskoilic humanembryonicstemcellsderivedfromembryosatdifferentstagesofdevelopmentsharesimilartranscriptionprofiles AT matthewgormley humanembryonicstemcellsderivedfromembryosatdifferentstagesofdevelopmentsharesimilartranscriptionprofiles AT anakrtolica humanembryonicstemcellsderivedfromembryosatdifferentstagesofdevelopmentsharesimilartranscriptionprofiles
_version_	1718423225145229312

Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles.

Ejemplares similares