Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles

Abstract Glaucoma is a major cause of blindness and is frequently associated with elevated intraocular pressure. The trabecular meshwork (TM), the tissue that primarily regulates intraocular pressure, is known to have reduced cellularity in glaucoma. Thus, stem cells, if properly delivered to the TM...

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Autores principales:	E. J. Snider, K. P. Kubelick, K. Tweed, R. K. Kim, Y. Li, K. Gao, A. T. Read, S. Emelianov, C. R. Ethier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6647e12fda074f039ae079b15de63faa
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spelling	oai:doaj.org-article:6647e12fda074f039ae079b15de63faa2021-12-02T15:08:22ZImproving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles10.1038/s41598-018-30834-72045-2322https://doaj.org/article/6647e12fda074f039ae079b15de63faa2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30834-7https://doaj.org/toc/2045-2322Abstract Glaucoma is a major cause of blindness and is frequently associated with elevated intraocular pressure. The trabecular meshwork (TM), the tissue that primarily regulates intraocular pressure, is known to have reduced cellularity in glaucoma. Thus, stem cells, if properly delivered to the TM, may offer a novel therapeutic option for intraocular pressure control in glaucoma patients. For this purpose, targeted delivery of stem cells to the TM is desired. Here, we used magnetic nanoparticles (Prussian blue nanocubes [PBNCs]) to label mesenchymal stem cells and to magnetically steer them to the TM following injection into the eye’s anterior chamber. PBNC-labeled stem cells showed increased delivery to the TM vs. unlabeled cells after only 15-minute exposure to a magnetic field. Further, PBNC-labeled mesenchymal stem cells could be delivered to the entire circumference of the TM, which was not possible without magnetic steering. PBNCs did not affect mesenchymal stem cell viability or multipotency. We conclude that this labeling approach allows for targeted, relatively high-efficiency delivery of stem cells to the TM in clinically translatable time-scales, which are necessary steps towards regenerative medicine therapies for control of ocular hypertension in glaucoma patients.E. J. SniderK. P. KubelickK. TweedR. K. KimY. LiK. GaoA. T. ReadS. EmelianovC. R. EthierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. J. Snider K. P. Kubelick K. Tweed R. K. Kim Y. Li K. Gao A. T. Read S. Emelianov C. R. Ethier Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
description	Abstract Glaucoma is a major cause of blindness and is frequently associated with elevated intraocular pressure. The trabecular meshwork (TM), the tissue that primarily regulates intraocular pressure, is known to have reduced cellularity in glaucoma. Thus, stem cells, if properly delivered to the TM, may offer a novel therapeutic option for intraocular pressure control in glaucoma patients. For this purpose, targeted delivery of stem cells to the TM is desired. Here, we used magnetic nanoparticles (Prussian blue nanocubes [PBNCs]) to label mesenchymal stem cells and to magnetically steer them to the TM following injection into the eye’s anterior chamber. PBNC-labeled stem cells showed increased delivery to the TM vs. unlabeled cells after only 15-minute exposure to a magnetic field. Further, PBNC-labeled mesenchymal stem cells could be delivered to the entire circumference of the TM, which was not possible without magnetic steering. PBNCs did not affect mesenchymal stem cell viability or multipotency. We conclude that this labeling approach allows for targeted, relatively high-efficiency delivery of stem cells to the TM in clinically translatable time-scales, which are necessary steps towards regenerative medicine therapies for control of ocular hypertension in glaucoma patients.
format	article
author	E. J. Snider K. P. Kubelick K. Tweed R. K. Kim Y. Li K. Gao A. T. Read S. Emelianov C. R. Ethier
author_facet	E. J. Snider K. P. Kubelick K. Tweed R. K. Kim Y. Li K. Gao A. T. Read S. Emelianov C. R. Ethier
author_sort	E. J. Snider
title	Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
title_short	Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
title_full	Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
title_fullStr	Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
title_full_unstemmed	Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles
title_sort	improving stem cell delivery to the trabecular meshwork using magnetic nanoparticles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6647e12fda074f039ae079b15de63faa
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Improving Stem Cell Delivery to the Trabecular Meshwork Using Magnetic Nanoparticles

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