Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.

Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of...

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Autores principales:	Reem M Hassan, Sherifa T Salem, Saly Ismail Mostafa Hassan, Asmaa Sayed Hegab, Yasmine S Elkholy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/664f5ccf02594a93a0af9300120a4c75
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spelling	oai:doaj.org-article:664f5ccf02594a93a0af9300120a4c752021-12-02T20:05:17ZMolecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.1932-620310.1371/journal.pone.0251508https://doaj.org/article/664f5ccf02594a93a0af9300120a4c752021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251508https://doaj.org/toc/1932-6203Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of ß-lactamases, have been identified as the most common mechanism of carbapenem resistance in A. baumannii. The alarming increase in the prevalence of CR-AB necessitates continuous screening and molecular characterization to appreciate the problem. The present study was performed to assess the prevalence and characterize carbapenemases among 206 CR-AB isolated from various clinical specimens collected from different intensive care units at Kasr Al-Aini Hospital. All isolates were confirmed to be A. baumannii by detection of the blaOXA-51-like gene. Molecular screening of 13 common Ambler class bla carbapenemases genes in addition to insertion sequence (IS-1) upstream OXA-23 were performed by using four sets of multiplex PCR, followed by identification using gene sequencing technology. Among the investigated genes, the prevalence of blaOXA-23, and blaOXA-58 were 77.7%, and 1.9%, respectively. The ISAba1 was detected in 10% of the blaOXA-23 positive isolates. The prevalence of metallo-β-lactamases (MBLs) studied; blaNDM-1, blaSPM, blaVIM, blaSIM-1 were 11.7%, 6.3%, 0.5%, and 0.5% respectively. One of class A; bla KPC was detected in 10.7% of the investigated isolates. blaOXA-24/40, blaIMP, blaGES, blaVEB and blaGIM were not detected in any of the studied isolates. Moreover, 18.4% of the isolates have shown to harbor two or more of the screened bla genes. We concluded that the most prevalent type of ß-lactamases genes among CR-AB isolates collected from Egyptian patients were blaOXA-23 followed by blaNDM-1 and blaKPC.Reem M HassanSherifa T SalemSaly Ismail Mostafa HassanAsmaa Sayed HegabYasmine S ElkholyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251508 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reem M Hassan Sherifa T Salem Saly Ismail Mostafa Hassan Asmaa Sayed Hegab Yasmine S Elkholy Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
description	Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of ß-lactamases, have been identified as the most common mechanism of carbapenem resistance in A. baumannii. The alarming increase in the prevalence of CR-AB necessitates continuous screening and molecular characterization to appreciate the problem. The present study was performed to assess the prevalence and characterize carbapenemases among 206 CR-AB isolated from various clinical specimens collected from different intensive care units at Kasr Al-Aini Hospital. All isolates were confirmed to be A. baumannii by detection of the blaOXA-51-like gene. Molecular screening of 13 common Ambler class bla carbapenemases genes in addition to insertion sequence (IS-1) upstream OXA-23 were performed by using four sets of multiplex PCR, followed by identification using gene sequencing technology. Among the investigated genes, the prevalence of blaOXA-23, and blaOXA-58 were 77.7%, and 1.9%, respectively. The ISAba1 was detected in 10% of the blaOXA-23 positive isolates. The prevalence of metallo-β-lactamases (MBLs) studied; blaNDM-1, blaSPM, blaVIM, blaSIM-1 were 11.7%, 6.3%, 0.5%, and 0.5% respectively. One of class A; bla KPC was detected in 10.7% of the investigated isolates. blaOXA-24/40, blaIMP, blaGES, blaVEB and blaGIM were not detected in any of the studied isolates. Moreover, 18.4% of the isolates have shown to harbor two or more of the screened bla genes. We concluded that the most prevalent type of ß-lactamases genes among CR-AB isolates collected from Egyptian patients were blaOXA-23 followed by blaNDM-1 and blaKPC.
format	article
author	Reem M Hassan Sherifa T Salem Saly Ismail Mostafa Hassan Asmaa Sayed Hegab Yasmine S Elkholy
author_facet	Reem M Hassan Sherifa T Salem Saly Ismail Mostafa Hassan Asmaa Sayed Hegab Yasmine S Elkholy
author_sort	Reem M Hassan
title	Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
title_short	Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
title_full	Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
title_fullStr	Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
title_full_unstemmed	Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.
title_sort	molecular characterization of carbapenem-resistant acinetobacter baumannii clinical isolates from egyptian patients.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/664f5ccf02594a93a0af9300120a4c75
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Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients.

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