Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4

Hepatitis E virus (HEV) belongs to the family Hepeviridae and is the major cause of hepatitis E infections across the globe. Recently, a novel viral protein of HEV, named as open reading frame (ORF4), has been associated with its replication in genotype 1 isolates. However, much information regardin...

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Autores principales:	Zoya Shafat, Ayesha Tazeen, Murshad Ahmed, et al.
Formato:	article
Lenguaje:	EN
Publicado:	International Academy of Ecology and Environmental Sciences 2021
Materias:	hepatitis e virus open reading frame (orf4) physicochemical parameters structural analysis functional analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/665096b3b9ff4a1b8dc275270a6282e2
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spelling	oai:doaj.org-article:665096b3b9ff4a1b8dc275270a6282e22021-11-17T03:38:26ZUnderstanding Hepatitis E Viruses by exploring the structural and functional properties of ORF42220-8879https://doaj.org/article/665096b3b9ff4a1b8dc275270a6282e22021-12-01T00:00:00Zhttp://www.iaees.org/publications/journals/nb/articles/2021-11(4)/structural-and-functional-properties-of-ORF4-Hepatitis-virus.pdfhttps://doaj.org/toc/2220-8879Hepatitis E virus (HEV) belongs to the family Hepeviridae and is the major cause of hepatitis E infections across the globe. Recently, a novel viral protein of HEV, named as open reading frame (ORF4), has been associated with its replication in genotype 1 isolates. However, much information regarding ORF4 has not been explored. Thus, the study was conceptualized to explore the structural and functional features of HEV ORF4 protein to better understand the possible molecular mechanisms. The detailed investigation of the ORF4 was carried out in terms of its physicochemical properties, secondary and tertiary structure predictions and functional analysis using different bioinformatics tools. The in silico analyses revealed that ORF4 sequences were enriched in Serine, Proline and Glycine amino acid residues suggesting the prevalence of disordered residues. The protein was found to be thermostable, unstable and highly hydrophobic. The structural analysis showed the presence of cleft, tunnel and pore suggesting their participation in interaction with other molecules. Moreover, identification of several modified sites in ORF4 sequences such as glycosylation, phosphorylation and myristoylation sequences suggest their involvement in cellular signaling pathways and biological processes. Thus, taken together, it can be interpreted that HEV ORF4 possesses significant enormous flexibility due to the presence of Serine, Glycine and Proline amino acids, which suggest its involvement in protein-protein interaction. Furthermore, the presence of motifs, clefts and tunnels also strengthens our analysis, suggesting the commitment of ORF4 towards interaction with other target molecules. Thus, it could be potent drug-targets.Zoya ShafatAyesha TazeenMurshad Ahmed, et al.International Academy of Ecology and Environmental Sciencesarticlehepatitis e virusopen reading frame (orf4)physicochemical parametersstructural analysisfunctional analysisBiology (General)QH301-705.5ENNetwork Biology, Vol 11, Iss 4, Pp 277-294 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis e virus open reading frame (orf4) physicochemical parameters structural analysis functional analysis Biology (General) QH301-705.5
spellingShingle	hepatitis e virus open reading frame (orf4) physicochemical parameters structural analysis functional analysis Biology (General) QH301-705.5 Zoya Shafat Ayesha Tazeen Murshad Ahmed, et al. Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
description	Hepatitis E virus (HEV) belongs to the family Hepeviridae and is the major cause of hepatitis E infections across the globe. Recently, a novel viral protein of HEV, named as open reading frame (ORF4), has been associated with its replication in genotype 1 isolates. However, much information regarding ORF4 has not been explored. Thus, the study was conceptualized to explore the structural and functional features of HEV ORF4 protein to better understand the possible molecular mechanisms. The detailed investigation of the ORF4 was carried out in terms of its physicochemical properties, secondary and tertiary structure predictions and functional analysis using different bioinformatics tools. The in silico analyses revealed that ORF4 sequences were enriched in Serine, Proline and Glycine amino acid residues suggesting the prevalence of disordered residues. The protein was found to be thermostable, unstable and highly hydrophobic. The structural analysis showed the presence of cleft, tunnel and pore suggesting their participation in interaction with other molecules. Moreover, identification of several modified sites in ORF4 sequences such as glycosylation, phosphorylation and myristoylation sequences suggest their involvement in cellular signaling pathways and biological processes. Thus, taken together, it can be interpreted that HEV ORF4 possesses significant enormous flexibility due to the presence of Serine, Glycine and Proline amino acids, which suggest its involvement in protein-protein interaction. Furthermore, the presence of motifs, clefts and tunnels also strengthens our analysis, suggesting the commitment of ORF4 towards interaction with other target molecules. Thus, it could be potent drug-targets.
format	article
author	Zoya Shafat Ayesha Tazeen Murshad Ahmed, et al.
author_facet	Zoya Shafat Ayesha Tazeen Murshad Ahmed, et al.
author_sort	Zoya Shafat
title	Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
title_short	Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
title_full	Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
title_fullStr	Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
title_full_unstemmed	Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4
title_sort	understanding hepatitis e viruses by exploring the structural and functional properties of orf4
publisher	International Academy of Ecology and Environmental Sciences
publishDate	2021
url	https://doaj.org/article/665096b3b9ff4a1b8dc275270a6282e2
work_keys_str_mv	AT zoyashafat understandinghepatitisevirusesbyexploringthestructuralandfunctionalpropertiesoforf4 AT ayeshatazeen understandinghepatitisevirusesbyexploringthestructuralandfunctionalpropertiesoforf4 AT murshadahmedetal understandinghepatitisevirusesbyexploringthestructuralandfunctionalpropertiesoforf4
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Understanding Hepatitis E Viruses by exploring the structural and functional properties of ORF4

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