Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Murali M Yallapu1, Shadi F Othman2, Evan T Curtis2, Nichole A Bauer1, Neeraj Chauhan1,3, Deepak Kumar4,5, Meena Jaggi1,3,6, Subhash C Chauhan1,3,61Cancer Biology Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD, 2Department of Biological Systems Engineering, University o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yallapu MM, Othman SF, Curtis ET, Bauer NA, Chauhan N, Kumar D, Jaggi M, Chauhan SC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6654dc0f7c8d489c8c2aeeca44fd98f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6654dc0f7c8d489c8c2aeeca44fd98f4
record_format	dspace
spelling	oai:doaj.org-article:6654dc0f7c8d489c8c2aeeca44fd98f42021-12-02T07:28:31ZCurcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications1176-91141178-2013https://doaj.org/article/6654dc0f7c8d489c8c2aeeca44fd98f42012-04-01T00:00:00Zhttp://www.dovepress.com/curcumin-loaded-magnetic-nanoparticles-for-breast-cancer-therapeutics--a9709https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Murali M Yallapu1, Shadi F Othman2, Evan T Curtis2, Nichole A Bauer1, Neeraj Chauhan1,3, Deepak Kumar4,5, Meena Jaggi1,3,6, Subhash C Chauhan1,3,61Cancer Biology Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD, 2Department of Biological Systems Engineering, University of Nebraska&ndash;Lincoln, Lincoln, NE, 3Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, 4Cancer Research Laboratory, Department of Biology, University of the District of Columbia, 5Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, 6Department of Obstetrics/Gynecology, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USABackground: The next generation magnetic nanoparticles (MNPs) with theranostic applications have attracted significant attention and will greatly improve nanomedicine in cancer therapeutics. Such novel MNP formulations must have ultra-low particle size, high inherent magnetic properties, effective imaging, drug targeting, and drug delivery properties. To achieve these characteristic properties, a curcumin-loaded MNP (MNP-CUR) formulation was developed.Methods: MNPs were prepared by chemical precipitation method and loaded with curcumin (CUR) using diffusion method. The physicochemical properties of MNP-CUR were characterized using dynamic light scattering, transmission electron microscopy, and spectroscopy. The internalization of MNP-CUR was achieved after 6 hours incubation with MDA-MB-231 breast cancer cells. The anticancer potential was evaluated by a tetrazolium-based dye and colony formation assays. Further, to prove MNP-CUR results in superior therapeutic effects over CUR, the mitochondrial membrane potential integrity and reactive oxygen species generation were determined. Magnetic resonance imaging capability and magnetic targeting property were also evaluated.Results: MNP-CUR exhibited individual particle grain size of ~9 nm and hydrodynamic average aggregative particle size of ~123 nm. Internalized MNP-CUR showed a preferential uptake in MDA-MB-231 cells in a concentration-dependent manner and demonstrated accumulation throughout the cell, which indicates that particles are not attached on the cell surface but internalized through endocytosis. MNP-CUR displayed strong anticancer properties compared to free CUR. MNP-CUR also amplified loss of potential integrity and generation of reactive oxygen species upon treatment compared to free CUR. Furthermore, MNP-CUR exhibited superior magnetic resonance imaging characteristics and significantly increased the targeting capability of CUR.Conclusion: MNP-CUR exhibits potent anticancer activity along with imaging and magnetic targeting capabilities. This approach can be extended to preclinical and clinical use and may have importance in cancer treatment and cancer imaging in the future. Further, if these nanoparticles can functionalize with antibody/ligands, they will serve as novel platforms for multiple biomedical applications.Keywords: magnetic nanoparticles, drug delivery systems, magnetic resonance imaging, nanomedicine, cancer therapeutics, biomedical applicationsYallapu MMOthman SFCurtis ETBauer NAChauhan NKumar DJaggi MChauhan SCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1761-1779 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yallapu MM Othman SF Curtis ET Bauer NA Chauhan N Kumar D Jaggi M Chauhan SC Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
description	Murali M Yallapu1, Shadi F Othman2, Evan T Curtis2, Nichole A Bauer1, Neeraj Chauhan1,3, Deepak Kumar4,5, Meena Jaggi1,3,6, Subhash C Chauhan1,3,61Cancer Biology Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD, 2Department of Biological Systems Engineering, University of Nebraska&ndash;Lincoln, Lincoln, NE, 3Basic Biomedical Science Division, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, 4Cancer Research Laboratory, Department of Biology, University of the District of Columbia, 5Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, 6Department of Obstetrics/Gynecology, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USABackground: The next generation magnetic nanoparticles (MNPs) with theranostic applications have attracted significant attention and will greatly improve nanomedicine in cancer therapeutics. Such novel MNP formulations must have ultra-low particle size, high inherent magnetic properties, effective imaging, drug targeting, and drug delivery properties. To achieve these characteristic properties, a curcumin-loaded MNP (MNP-CUR) formulation was developed.Methods: MNPs were prepared by chemical precipitation method and loaded with curcumin (CUR) using diffusion method. The physicochemical properties of MNP-CUR were characterized using dynamic light scattering, transmission electron microscopy, and spectroscopy. The internalization of MNP-CUR was achieved after 6 hours incubation with MDA-MB-231 breast cancer cells. The anticancer potential was evaluated by a tetrazolium-based dye and colony formation assays. Further, to prove MNP-CUR results in superior therapeutic effects over CUR, the mitochondrial membrane potential integrity and reactive oxygen species generation were determined. Magnetic resonance imaging capability and magnetic targeting property were also evaluated.Results: MNP-CUR exhibited individual particle grain size of ~9 nm and hydrodynamic average aggregative particle size of ~123 nm. Internalized MNP-CUR showed a preferential uptake in MDA-MB-231 cells in a concentration-dependent manner and demonstrated accumulation throughout the cell, which indicates that particles are not attached on the cell surface but internalized through endocytosis. MNP-CUR displayed strong anticancer properties compared to free CUR. MNP-CUR also amplified loss of potential integrity and generation of reactive oxygen species upon treatment compared to free CUR. Furthermore, MNP-CUR exhibited superior magnetic resonance imaging characteristics and significantly increased the targeting capability of CUR.Conclusion: MNP-CUR exhibits potent anticancer activity along with imaging and magnetic targeting capabilities. This approach can be extended to preclinical and clinical use and may have importance in cancer treatment and cancer imaging in the future. Further, if these nanoparticles can functionalize with antibody/ligands, they will serve as novel platforms for multiple biomedical applications.Keywords: magnetic nanoparticles, drug delivery systems, magnetic resonance imaging, nanomedicine, cancer therapeutics, biomedical applications
format	article
author	Yallapu MM Othman SF Curtis ET Bauer NA Chauhan N Kumar D Jaggi M Chauhan SC
author_facet	Yallapu MM Othman SF Curtis ET Bauer NA Chauhan N Kumar D Jaggi M Chauhan SC
author_sort	Yallapu MM
title	Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
title_short	Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
title_full	Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
title_fullStr	Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
title_full_unstemmed	Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
title_sort	curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/6654dc0f7c8d489c8c2aeeca44fd98f4
work_keys_str_mv	AT yallapumm curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT othmansf curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT curtiset curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT bauerna curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT chauhann curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT kumard curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT jaggim curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications AT chauhansc curcuminloadedmagneticnanoparticlesforbreastcancertherapeuticsandimagingapplications
_version_	1718399405950763008

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Ejemplares similares