Molecular architecture of black widow spider neurotoxins

The venom of Latrodectus spiders contains seven Latrotoxins (LaTXs), among them α-latrocrustatoxin (LCT) and δ- latroinsectotoxins δ-LIT. LaTXs bind to specific receptors on the surface of neuronal cells and target the molecular exocytosis machinery. Here, the authors present the cryo-EM structure o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Minghao Chen, Daniel Blum, Lena Engelhard, Stefan Raunser, Richard Wagner, Christos Gatsogiannis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/665db54f53c6489eba8074903eca63cd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:665db54f53c6489eba8074903eca63cd
record_format	dspace
spelling	oai:doaj.org-article:665db54f53c6489eba8074903eca63cd2021-12-05T12:21:51ZMolecular architecture of black widow spider neurotoxins10.1038/s41467-021-26562-82041-1723https://doaj.org/article/665db54f53c6489eba8074903eca63cd2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26562-8https://doaj.org/toc/2041-1723The venom of Latrodectus spiders contains seven Latrotoxins (LaTXs), among them α-latrocrustatoxin (LCT) and δ- latroinsectotoxins δ-LIT. LaTXs bind to specific receptors on the surface of neuronal cells and target the molecular exocytosis machinery. Here, the authors present the cryo-EM structure of the α-LCT monomer and the δ-LIT dimer, which reveal that LaTXs are organized in four domains and they discuss the potential oligomerisation mechanism that takes place before LaTXs membrane insertion. Both recombinant α-LCT and δ-LIT form channels in artificial membrane bilayers, that are stabilized by Ca2+ ions.Minghao ChenDaniel BlumLena EngelhardStefan RaunserRichard WagnerChristos GatsogiannisNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Minghao Chen Daniel Blum Lena Engelhard Stefan Raunser Richard Wagner Christos Gatsogiannis Molecular architecture of black widow spider neurotoxins
description	The venom of Latrodectus spiders contains seven Latrotoxins (LaTXs), among them α-latrocrustatoxin (LCT) and δ- latroinsectotoxins δ-LIT. LaTXs bind to specific receptors on the surface of neuronal cells and target the molecular exocytosis machinery. Here, the authors present the cryo-EM structure of the α-LCT monomer and the δ-LIT dimer, which reveal that LaTXs are organized in four domains and they discuss the potential oligomerisation mechanism that takes place before LaTXs membrane insertion. Both recombinant α-LCT and δ-LIT form channels in artificial membrane bilayers, that are stabilized by Ca2+ ions.
format	article
author	Minghao Chen Daniel Blum Lena Engelhard Stefan Raunser Richard Wagner Christos Gatsogiannis
author_facet	Minghao Chen Daniel Blum Lena Engelhard Stefan Raunser Richard Wagner Christos Gatsogiannis
author_sort	Minghao Chen
title	Molecular architecture of black widow spider neurotoxins
title_short	Molecular architecture of black widow spider neurotoxins
title_full	Molecular architecture of black widow spider neurotoxins
title_fullStr	Molecular architecture of black widow spider neurotoxins
title_full_unstemmed	Molecular architecture of black widow spider neurotoxins
title_sort	molecular architecture of black widow spider neurotoxins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/665db54f53c6489eba8074903eca63cd
work_keys_str_mv	AT minghaochen moleculararchitectureofblackwidowspiderneurotoxins AT danielblum moleculararchitectureofblackwidowspiderneurotoxins AT lenaengelhard moleculararchitectureofblackwidowspiderneurotoxins AT stefanraunser moleculararchitectureofblackwidowspiderneurotoxins AT richardwagner moleculararchitectureofblackwidowspiderneurotoxins AT christosgatsogiannis moleculararchitectureofblackwidowspiderneurotoxins
_version_	1718372020989722624

Molecular architecture of black widow spider neurotoxins

Ejemplares similares