Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction

A lot of human activities have negative impact on water quality and sometimes result in the biological water contamination. Currently used chemical (chlorine, ozone, and etc.) and physical (UV) water disinfection methods have strong environmental disadvantages or suffers from limited efficiency. To...

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Autores principales:	Vasiliauske Deimante, Sakalauskaite Sandra, Kuliesiene Neringa, Tuckute Simona, Urbonavicius Marius, Varnagiris Sarunas, Daugelavicius Rimantas, Lelis Martynas
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	disinfection methylene blue photocatalysis salmonella enterica titanium dioxide uv light visible light Renewable energy sources TJ807-830
Acceso en línea:	https://doaj.org/article/6663874f1b5647819df4c7bc65e100b0
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spelling	oai:doaj.org-article:6663874f1b5647819df4c7bc65e100b02021-12-05T14:11:11ZSynergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction2255-883710.2478/rtuect-2021-0074https://doaj.org/article/6663874f1b5647819df4c7bc65e100b02021-01-01T00:00:00Zhttps://doi.org/10.2478/rtuect-2021-0074https://doaj.org/toc/2255-8837A lot of human activities have negative impact on water quality and sometimes result in the biological water contamination. Currently used chemical (chlorine, ozone, and etc.) and physical (UV) water disinfection methods have strong environmental disadvantages or suffers from limited efficiency. To overcome these problems, scientists suggest to use photocatalyst activated advanced oxidation processes. One of the most studied photocatalysts which attracts a lot of research interest is titanium dioxide. TiO2 application for the disinfection of water, air or surfaces is increasingly encouraged by researchers. However, to unlock its full potential it is highly desirable to make it suitable for the visible light activation. In the current study the effect of visible light assisted photocatalytic treatment to the outer membrane permeability of Salmonella enterica bacteria and how it changes under different titanium dioxide concentrations was analysed. The results from the treatment of relatively complex Salmonella enterica bacteria organism were compared to the visible light activated TiO2 ability to oxidise considerably simpler objects like methylene blue molecules. The efficiency of TiO2 photocatalytic disinfection process was evaluated using spread plate technique. Membrane permeability of the treated Salmonella enterica bacteria was determined by NPN uptake factor assay. Generation of intracellular reactive oxygen species was evaluated by Dichlorodihydrofluorescein diacetate fluorescence measurements. The key finding of this study was that intense wide spectrum visible light irradiation and TiO2 powder synergistically inactivate S. enterica bacteria and halt its potential to form colonies. High amounts of intracellular reactive oxygen species could be seen as the main suspects for the observed inactivation of S. enterica.Vasiliauske DeimanteSakalauskaite SandraKuliesiene NeringaTuckute SimonaUrbonavicius MariusVarnagiris SarunasDaugelavicius RimantasLelis MartynasSciendoarticledisinfectionmethylene bluephotocatalysissalmonella entericatitanium dioxideuv lightvisible lightRenewable energy sourcesTJ807-830ENEnvironmental and Climate Technologies, Vol 25, Iss 1, Pp 978-989 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	disinfection methylene blue photocatalysis salmonella enterica titanium dioxide uv light visible light Renewable energy sources TJ807-830
spellingShingle	disinfection methylene blue photocatalysis salmonella enterica titanium dioxide uv light visible light Renewable energy sources TJ807-830 Vasiliauske Deimante Sakalauskaite Sandra Kuliesiene Neringa Tuckute Simona Urbonavicius Marius Varnagiris Sarunas Daugelavicius Rimantas Lelis Martynas Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
description	A lot of human activities have negative impact on water quality and sometimes result in the biological water contamination. Currently used chemical (chlorine, ozone, and etc.) and physical (UV) water disinfection methods have strong environmental disadvantages or suffers from limited efficiency. To overcome these problems, scientists suggest to use photocatalyst activated advanced oxidation processes. One of the most studied photocatalysts which attracts a lot of research interest is titanium dioxide. TiO2 application for the disinfection of water, air or surfaces is increasingly encouraged by researchers. However, to unlock its full potential it is highly desirable to make it suitable for the visible light activation. In the current study the effect of visible light assisted photocatalytic treatment to the outer membrane permeability of Salmonella enterica bacteria and how it changes under different titanium dioxide concentrations was analysed. The results from the treatment of relatively complex Salmonella enterica bacteria organism were compared to the visible light activated TiO2 ability to oxidise considerably simpler objects like methylene blue molecules. The efficiency of TiO2 photocatalytic disinfection process was evaluated using spread plate technique. Membrane permeability of the treated Salmonella enterica bacteria was determined by NPN uptake factor assay. Generation of intracellular reactive oxygen species was evaluated by Dichlorodihydrofluorescein diacetate fluorescence measurements. The key finding of this study was that intense wide spectrum visible light irradiation and TiO2 powder synergistically inactivate S. enterica bacteria and halt its potential to form colonies. High amounts of intracellular reactive oxygen species could be seen as the main suspects for the observed inactivation of S. enterica.
format	article
author	Vasiliauske Deimante Sakalauskaite Sandra Kuliesiene Neringa Tuckute Simona Urbonavicius Marius Varnagiris Sarunas Daugelavicius Rimantas Lelis Martynas
author_facet	Vasiliauske Deimante Sakalauskaite Sandra Kuliesiene Neringa Tuckute Simona Urbonavicius Marius Varnagiris Sarunas Daugelavicius Rimantas Lelis Martynas
author_sort	Vasiliauske Deimante
title	Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
title_short	Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
title_full	Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
title_fullStr	Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
title_full_unstemmed	Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction
title_sort	synergistic generation of reactive oxygen species by visible light activated tio2 and s. enterica interaction
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/6663874f1b5647819df4c7bc65e100b0
work_keys_str_mv	AT vasiliauskedeimante synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT sakalauskaitesandra synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT kuliesieneneringa synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT tuckutesimona synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT urbonaviciusmarius synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT varnagirissarunas synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT daugelaviciusrimantas synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction AT lelismartynas synergisticgenerationofreactiveoxygenspeciesbyvisiblelightactivatedtio2andsentericainteraction
_version_	1718371302742425600

Synergistic Generation of Reactive Oxygen Species by Visible Light Activated TiO2 and S. Enterica Interaction

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