Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects

Song Yang, 1, 2,* Gaohua Han, 1, 2,* Quan Chen, 2, 3 Lei Yu, 1, 2 Peng Wang, 1, 2 Qi Zhang, 1, 2 Jiang Dong, 1, 2 Wei Zhang, 2, 4 Junxing Huang 1, 2 1Department of Oncology, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 2Medical School of Nantong Univ...

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Autores principales:	Yang S, Han G, Chen Q, Yu L, Wang P, Zhang Q, Dong J, Zhang W, Huang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	radiosensitizer nano-enzyme tme radiotherapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6663eb0fc532468799d1422cd7517ade
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spelling	oai:doaj.org-article:6663eb0fc532468799d1422cd7517ade2021-12-02T13:36:08ZAu-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects1178-2013https://doaj.org/article/6663eb0fc532468799d1422cd7517ade2021-01-01T00:00:00Zhttps://www.dovepress.com/au-pt-nanoparticle-formulation-as-a-radiosensitizer-for-radiotherapy-w-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Song Yang, 1, 2,* Gaohua Han, 1, 2,* Quan Chen, 2, 3 Lei Yu, 1, 2 Peng Wang, 1, 2 Qi Zhang, 1, 2 Jiang Dong, 1, 2 Wei Zhang, 2, 4 Junxing Huang 1, 2 1Department of Oncology, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 2Medical School of Nantong University, Nantong, Jiangsu, People’s Republic of China; 3Department of Thoracic Surgery, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 4Department of Infectious Disease, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei ZhangDepartment of Infectious Disease, Taizhou People’s Hospital, No. 399, South Hailing Road, Taizhou, 225300 Jiangsu, People’s Republic of ChinaTel +86-523-86606739Email zw562652291@126.comJunxing HuangDepartment of Oncology, Taizhou People’s Hospital, No. 399, South Hailing Road, Taizhou, 225300 Jiangsu, People’s Republic of ChinaTel +86-523-86606739Email hjxtz@sina.cnBackground: Radiotherapy occupies an essential position as one of the most significant approaches for the clinical treatment of cancer. However, we cannot overcome the shortcoming of X-rays which is the high value of the oxygen enhancement ratio (OER). Radiosensitizers with the ability to enhance the radiosensitivity of tumor cells provide an alternative to changing X-rays to protons and heavy ion radiotherapy.Materials and Methods: We prepared the Au-Pt nanoparticles (Au-Pt NPs) using a one-step method. The characteristics of the Au-Pt NPs were determined using TEM, HAADF-STEM, elemental mapping images, and DLS. The enhanced radiotherapy was demonstrated in vitro using MTT assays, colony formation assays, fluorescence imaging, and flow cytometric analyses of the apoptosis. The biodistribution of the Au-Pt NPs was analyzed using ICP-OES, and thermal images. The enhanced radiotherapy was demonstrated in vitro using immunofluorescence images, tumor volume and weigh, and hematoxylin & eosin (H&E) staining.Results: Polyethylene glycol (PEG) functionalized nanoparticles composed of the metallic elements Au and Pt were designed to increase synergistic radiosensitivity. The mechanism demonstrated that heavy metal NPs possess a high X-ray photon capture cross-section and Compton scattering effect which increased DNA damage. Furthermore, the Au-Pt NPs exhibited enzyme-mimicking activities by catalyzing the decomposition of endogenous H 2O 2 to O 2 in the solid tumor microenvironment (TME).Conclusion: Our work provides a systematically administered radiosensitizer that can selectively reside in a tumor via the EPR effect and enhances the efficiency of treating cancer with radiotherapy.Keywords: radiosensitizer, nano-enzyme, TME, radiotherapyYang SHan GChen QYu LWang PZhang QDong JZhang WHuang JDove Medical Pressarticleradiosensitizernano-enzymetmeradiotherapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 239-248 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	radiosensitizer nano-enzyme tme radiotherapy Medicine (General) R5-920
spellingShingle	radiosensitizer nano-enzyme tme radiotherapy Medicine (General) R5-920 Yang S Han G Chen Q Yu L Wang P Zhang Q Dong J Zhang W Huang J Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
description	Song Yang, 1, 2,* Gaohua Han, 1, 2,* Quan Chen, 2, 3 Lei Yu, 1, 2 Peng Wang, 1, 2 Qi Zhang, 1, 2 Jiang Dong, 1, 2 Wei Zhang, 2, 4 Junxing Huang 1, 2 1Department of Oncology, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 2Medical School of Nantong University, Nantong, Jiangsu, People’s Republic of China; 3Department of Thoracic Surgery, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 4Department of Infectious Disease, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei ZhangDepartment of Infectious Disease, Taizhou People’s Hospital, No. 399, South Hailing Road, Taizhou, 225300 Jiangsu, People’s Republic of ChinaTel +86-523-86606739Email zw562652291@126.comJunxing HuangDepartment of Oncology, Taizhou People’s Hospital, No. 399, South Hailing Road, Taizhou, 225300 Jiangsu, People’s Republic of ChinaTel +86-523-86606739Email hjxtz@sina.cnBackground: Radiotherapy occupies an essential position as one of the most significant approaches for the clinical treatment of cancer. However, we cannot overcome the shortcoming of X-rays which is the high value of the oxygen enhancement ratio (OER). Radiosensitizers with the ability to enhance the radiosensitivity of tumor cells provide an alternative to changing X-rays to protons and heavy ion radiotherapy.Materials and Methods: We prepared the Au-Pt nanoparticles (Au-Pt NPs) using a one-step method. The characteristics of the Au-Pt NPs were determined using TEM, HAADF-STEM, elemental mapping images, and DLS. The enhanced radiotherapy was demonstrated in vitro using MTT assays, colony formation assays, fluorescence imaging, and flow cytometric analyses of the apoptosis. The biodistribution of the Au-Pt NPs was analyzed using ICP-OES, and thermal images. The enhanced radiotherapy was demonstrated in vitro using immunofluorescence images, tumor volume and weigh, and hematoxylin & eosin (H&E) staining.Results: Polyethylene glycol (PEG) functionalized nanoparticles composed of the metallic elements Au and Pt were designed to increase synergistic radiosensitivity. The mechanism demonstrated that heavy metal NPs possess a high X-ray photon capture cross-section and Compton scattering effect which increased DNA damage. Furthermore, the Au-Pt NPs exhibited enzyme-mimicking activities by catalyzing the decomposition of endogenous H 2O 2 to O 2 in the solid tumor microenvironment (TME).Conclusion: Our work provides a systematically administered radiosensitizer that can selectively reside in a tumor via the EPR effect and enhances the efficiency of treating cancer with radiotherapy.Keywords: radiosensitizer, nano-enzyme, TME, radiotherapy
format	article
author	Yang S Han G Chen Q Yu L Wang P Zhang Q Dong J Zhang W Huang J
author_facet	Yang S Han G Chen Q Yu L Wang P Zhang Q Dong J Zhang W Huang J
author_sort	Yang S
title	Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
title_short	Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
title_full	Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
title_fullStr	Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
title_full_unstemmed	Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects
title_sort	au-pt nanoparticle formulation as a radiosensitizer for radiotherapy with dual effects
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/6663eb0fc532468799d1422cd7517ade
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Au-Pt Nanoparticle Formulation as a Radiosensitizer for Radiotherapy with Dual Effects

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