CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.

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Autores principales:	Mitchell G. Kluesner, Walker S. Lahr, Cara-lin Lonetree, Branden A. Smeester, Xiaohong Qiu, Nicholas J. Slipek, Patricia N. Claudio Vázquez, Samuel P. Pitzen, Emily J. Pomeroy, Madison J. Vignes, Samantha C. Lee, Samuel P. Bingea, Aneesha A. Andrew, Beau R. Webber, Branden S. Moriarity
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/66661828f7814bdf8c8c049d29a4b13b
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spelling	oai:doaj.org-article:66661828f7814bdf8c8c049d29a4b13b2021-12-02T13:39:33ZCRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells10.1038/s41467-021-22009-22041-1723https://doaj.org/article/66661828f7814bdf8c8c049d29a4b13b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22009-2https://doaj.org/toc/2041-1723Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.Mitchell G. KluesnerWalker S. LahrCara-lin LonetreeBranden A. SmeesterXiaohong QiuNicholas J. SlipekPatricia N. Claudio VázquezSamuel P. PitzenEmily J. PomeroyMadison J. VignesSamantha C. LeeSamuel P. BingeaAneesha A. AndrewBeau R. WebberBranden S. MoriarityNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mitchell G. Kluesner Walker S. Lahr Cara-lin Lonetree Branden A. Smeester Xiaohong Qiu Nicholas J. Slipek Patricia N. Claudio Vázquez Samuel P. Pitzen Emily J. Pomeroy Madison J. Vignes Samantha C. Lee Samuel P. Bingea Aneesha A. Andrew Beau R. Webber Branden S. Moriarity CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
description	Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.
format	article
author	Mitchell G. Kluesner Walker S. Lahr Cara-lin Lonetree Branden A. Smeester Xiaohong Qiu Nicholas J. Slipek Patricia N. Claudio Vázquez Samuel P. Pitzen Emily J. Pomeroy Madison J. Vignes Samantha C. Lee Samuel P. Bingea Aneesha A. Andrew Beau R. Webber Branden S. Moriarity
author_facet	Mitchell G. Kluesner Walker S. Lahr Cara-lin Lonetree Branden A. Smeester Xiaohong Qiu Nicholas J. Slipek Patricia N. Claudio Vázquez Samuel P. Pitzen Emily J. Pomeroy Madison J. Vignes Samantha C. Lee Samuel P. Bingea Aneesha A. Andrew Beau R. Webber Branden S. Moriarity
author_sort	Mitchell G. Kluesner
title	CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
title_short	CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
title_full	CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
title_fullStr	CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
title_full_unstemmed	CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
title_sort	crispr-cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66661828f7814bdf8c8c049d29a4b13b
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CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

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