Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics

Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are...

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Autores principales:	Maria G. Sande, Joana L. Rodrigues, Débora Ferreira, Carla J. Silva, Ligia R. Rodrigues
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biorecognition diagnosis biosensor pathogens aptamers antibodies Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/6668ca6eff644ba2b71fdd33915cfe98
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spelling	oai:doaj.org-article:6668ca6eff644ba2b71fdd33915cfe982021-11-25T16:54:59ZNovel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics10.3390/bios111104182079-6374https://doaj.org/article/6668ca6eff644ba2b71fdd33915cfe982021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/418https://doaj.org/toc/2079-6374Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient rapid diagnostics which can be easily administered at the point-of-care (POC) and at low cost without sacrificing reliability. Biosensors and other rapid POC diagnostic tools which require biorecognition elements to precisely identify the causative pathogen are being developed. The effectiveness of these devices is highly dependent on their biorecognition capabilities. Naturally occurring biorecognition elements include antibodies, bacteriophages and enzymes. Recently, modified molecules such as DNAzymes, peptide nucleic acids and molecules which suffer a selective screening like aptamers and peptides are gaining interest for their biorecognition capabilities and other advantages over purely natural ones, such as robustness and lower production costs. Antimicrobials with a broad-spectrum activity against pathogens, such as antibiotics, are also used in dual diagnostic and therapeutic strategies. Other successful pathogen identification strategies use chemical ligands, molecularly imprinted polymers and Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease. Herein, the latest developments regarding biorecognition elements and strategies to use them in the design of new biosensors for pathogens detection are reviewed.Maria G. SandeJoana L. RodriguesDébora FerreiraCarla J. SilvaLigia R. RodriguesMDPI AGarticlebiorecognitiondiagnosisbiosensorpathogensaptamersantibodiesBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 418, p 418 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biorecognition diagnosis biosensor pathogens aptamers antibodies Biotechnology TP248.13-248.65
spellingShingle	biorecognition diagnosis biosensor pathogens aptamers antibodies Biotechnology TP248.13-248.65 Maria G. Sande Joana L. Rodrigues Débora Ferreira Carla J. Silva Ligia R. Rodrigues Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
description	Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient rapid diagnostics which can be easily administered at the point-of-care (POC) and at low cost without sacrificing reliability. Biosensors and other rapid POC diagnostic tools which require biorecognition elements to precisely identify the causative pathogen are being developed. The effectiveness of these devices is highly dependent on their biorecognition capabilities. Naturally occurring biorecognition elements include antibodies, bacteriophages and enzymes. Recently, modified molecules such as DNAzymes, peptide nucleic acids and molecules which suffer a selective screening like aptamers and peptides are gaining interest for their biorecognition capabilities and other advantages over purely natural ones, such as robustness and lower production costs. Antimicrobials with a broad-spectrum activity against pathogens, such as antibiotics, are also used in dual diagnostic and therapeutic strategies. Other successful pathogen identification strategies use chemical ligands, molecularly imprinted polymers and Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease. Herein, the latest developments regarding biorecognition elements and strategies to use them in the design of new biosensors for pathogens detection are reviewed.
format	article
author	Maria G. Sande Joana L. Rodrigues Débora Ferreira Carla J. Silva Ligia R. Rodrigues
author_facet	Maria G. Sande Joana L. Rodrigues Débora Ferreira Carla J. Silva Ligia R. Rodrigues
author_sort	Maria G. Sande
title	Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
title_short	Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
title_full	Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
title_fullStr	Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
title_full_unstemmed	Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics
title_sort	novel biorecognition elements against pathogens in the design of state-of-the-art diagnostics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6668ca6eff644ba2b71fdd33915cfe98
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Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics

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