Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures

Using a high-throughput mitochondrial phenotyping platform to quantify multiple mitochondrial features among molecularly defined immune cell subtypes, we quantify the natural variation in mitochondrial DNA copy number (mtDNAcn), citrate synthase, and respiratory chain enzymatic activities in human n...

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Autores principales:	Shannon Rausser, Caroline Trumpff, Marlon A McGill, Alex Junker, Wei Wang, Siu-Hong Ho, Anika Mitchell, Kalpita R Karan, Catherine Monk, Suzanne C Segerstrom, Rebecca G Reed, Martin Picard
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	mitochondria leukocytes sexual dimorphism aging dynamic variation immunometabolism Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/66750d7ee5894ea68f42b14e43507f68
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spelling	oai:doaj.org-article:66750d7ee5894ea68f42b14e43507f682021-11-24T16:29:06ZMitochondrial phenotypes in purified human immune cell subtypes and cell mixtures10.7554/eLife.708992050-084Xe70899https://doaj.org/article/66750d7ee5894ea68f42b14e43507f682021-10-01T00:00:00Zhttps://elifesciences.org/articles/70899https://doaj.org/toc/2050-084XUsing a high-throughput mitochondrial phenotyping platform to quantify multiple mitochondrial features among molecularly defined immune cell subtypes, we quantify the natural variation in mitochondrial DNA copy number (mtDNAcn), citrate synthase, and respiratory chain enzymatic activities in human neutrophils, monocytes, B cells, and naïve and memory T lymphocyte subtypes. In mixed peripheral blood mononuclear cells (PBMCs) from the same individuals, we show to what extent mitochondrial measures are confounded by both cell type distributions and contaminating platelets. Cell subtype-specific measures among women and men spanning four decades of life indicate potential age- and sex-related differences, including an age-related elevation in mtDNAcn, which are masked or blunted in mixed PBMCs. Finally, a proof-of-concept, repeated-measures study in a single individual validates cell type differences and also reveals week-to-week changes in mitochondrial activities. Larger studies are required to validate and mechanistically extend these findings. These mitochondrial phenotyping data build upon established immunometabolic differences among leukocyte subpopulations, and provide foundational quantitative knowledge to develop interpretable blood-based assays of mitochondrial health.Shannon RausserCaroline TrumpffMarlon A McGillAlex JunkerWei WangSiu-Hong HoAnika MitchellKalpita R KaranCatherine MonkSuzanne C SegerstromRebecca G ReedMartin PicardeLife Sciences Publications Ltdarticlemitochondrialeukocytessexual dimorphismagingdynamic variationimmunometabolismMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mitochondria leukocytes sexual dimorphism aging dynamic variation immunometabolism Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	mitochondria leukocytes sexual dimorphism aging dynamic variation immunometabolism Medicine R Science Q Biology (General) QH301-705.5 Shannon Rausser Caroline Trumpff Marlon A McGill Alex Junker Wei Wang Siu-Hong Ho Anika Mitchell Kalpita R Karan Catherine Monk Suzanne C Segerstrom Rebecca G Reed Martin Picard Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
description	Using a high-throughput mitochondrial phenotyping platform to quantify multiple mitochondrial features among molecularly defined immune cell subtypes, we quantify the natural variation in mitochondrial DNA copy number (mtDNAcn), citrate synthase, and respiratory chain enzymatic activities in human neutrophils, monocytes, B cells, and naïve and memory T lymphocyte subtypes. In mixed peripheral blood mononuclear cells (PBMCs) from the same individuals, we show to what extent mitochondrial measures are confounded by both cell type distributions and contaminating platelets. Cell subtype-specific measures among women and men spanning four decades of life indicate potential age- and sex-related differences, including an age-related elevation in mtDNAcn, which are masked or blunted in mixed PBMCs. Finally, a proof-of-concept, repeated-measures study in a single individual validates cell type differences and also reveals week-to-week changes in mitochondrial activities. Larger studies are required to validate and mechanistically extend these findings. These mitochondrial phenotyping data build upon established immunometabolic differences among leukocyte subpopulations, and provide foundational quantitative knowledge to develop interpretable blood-based assays of mitochondrial health.
format	article
author	Shannon Rausser Caroline Trumpff Marlon A McGill Alex Junker Wei Wang Siu-Hong Ho Anika Mitchell Kalpita R Karan Catherine Monk Suzanne C Segerstrom Rebecca G Reed Martin Picard
author_facet	Shannon Rausser Caroline Trumpff Marlon A McGill Alex Junker Wei Wang Siu-Hong Ho Anika Mitchell Kalpita R Karan Catherine Monk Suzanne C Segerstrom Rebecca G Reed Martin Picard
author_sort	Shannon Rausser
title	Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
title_short	Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
title_full	Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
title_fullStr	Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
title_full_unstemmed	Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
title_sort	mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/66750d7ee5894ea68f42b14e43507f68
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Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures

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