Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

Intercellular communication in the decidua plays important roles in relaying information between the maternal and fetal systems in the maintenance of pregnancy and the transition to labor. To date, several studies have explored cell-cell communications in the decidua during different periods of preg...

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Autores principales:	Jingrui Huang, Weishe Zhang, Yanhua Zhao, Jingzhi Li, Mingkun Xie, Yang Lu, Qiaozhen Peng, Jiejie Zhang, Ping Li, Lei Dai
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	decidua delivery intercellular communication B cell receptor repertoire T cell receptor repertoire single-cell RNA sequencing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/667eb6ff40fc413a8c22d48e3b436755
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spelling	oai:doaj.org-article:667eb6ff40fc413a8c22d48e3b4367552021-11-05T08:29:25ZDeciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery2296-634X10.3389/fcell.2021.770621https://doaj.org/article/667eb6ff40fc413a8c22d48e3b4367552021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.770621/fullhttps://doaj.org/toc/2296-634XIntercellular communication in the decidua plays important roles in relaying information between the maternal and fetal systems in the maintenance of pregnancy and the transition to labor. To date, several studies have explored cell-cell communications in the decidua during different periods of pregnancy, but studies systematically decoding the intercellular communication network, its internal cascades, and their involvement in labor are still lacking. In this study, we reconstructed a decidual cell-cell communication network based on scRNA-seq of peripartum decidua via the CellCall method. The results showed that endometrial cells (EECs) and extravillous trophoblasts relayed most of the common intercellular signals in the decidua both before delivery (DBD) and after delivery (DAD). Endothelial cells and EECs controlled many WNT-signaling-related intercellular communication factors that differed between DBD and DAD, some of which could be candidate biomarkers for the diagnosis of labor. Analysis of intercellular communications related to T cells identified abundant maternal-fetal immune-tolerance-related communication, such as TNFSF14-TNFRSF14/LTBR and FASLG-FAS signalings. We further explored the characteristics of the B cell receptor (BCR) and T cell receptor (TCR) repertoires by single-cell BCR/TCR sequencing. The results showed no significant differences in clonal expansion of B/T cells between DAD and DBD, indicating there was no significant change to adaptive immunity at the maternal-fetal interface during delivery. In summary, the findings provide a comprehensive view of the intercellular communication landscape in the peripartum decidua and identified some key intercellular communications involved in labor and maternal-fetal immune tolerance. We believe that our study provides valuable clues for understanding the mechanisms of pregnancy and provides possible diagnostic strategies for the onset of labor.Jingrui HuangWeishe ZhangWeishe ZhangYanhua ZhaoJingzhi LiMingkun XieYang LuQiaozhen PengJiejie ZhangPing LiLei DaiLei DaiFrontiers Media S.A.articledeciduadeliveryintercellular communicationB cell receptor repertoireT cell receptor repertoiresingle-cell RNA sequencingBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	decidua delivery intercellular communication B cell receptor repertoire T cell receptor repertoire single-cell RNA sequencing Biology (General) QH301-705.5
spellingShingle	decidua delivery intercellular communication B cell receptor repertoire T cell receptor repertoire single-cell RNA sequencing Biology (General) QH301-705.5 Jingrui Huang Weishe Zhang Weishe Zhang Yanhua Zhao Jingzhi Li Mingkun Xie Yang Lu Qiaozhen Peng Jiejie Zhang Ping Li Lei Dai Lei Dai Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
description	Intercellular communication in the decidua plays important roles in relaying information between the maternal and fetal systems in the maintenance of pregnancy and the transition to labor. To date, several studies have explored cell-cell communications in the decidua during different periods of pregnancy, but studies systematically decoding the intercellular communication network, its internal cascades, and their involvement in labor are still lacking. In this study, we reconstructed a decidual cell-cell communication network based on scRNA-seq of peripartum decidua via the CellCall method. The results showed that endometrial cells (EECs) and extravillous trophoblasts relayed most of the common intercellular signals in the decidua both before delivery (DBD) and after delivery (DAD). Endothelial cells and EECs controlled many WNT-signaling-related intercellular communication factors that differed between DBD and DAD, some of which could be candidate biomarkers for the diagnosis of labor. Analysis of intercellular communications related to T cells identified abundant maternal-fetal immune-tolerance-related communication, such as TNFSF14-TNFRSF14/LTBR and FASLG-FAS signalings. We further explored the characteristics of the B cell receptor (BCR) and T cell receptor (TCR) repertoires by single-cell BCR/TCR sequencing. The results showed no significant differences in clonal expansion of B/T cells between DAD and DBD, indicating there was no significant change to adaptive immunity at the maternal-fetal interface during delivery. In summary, the findings provide a comprehensive view of the intercellular communication landscape in the peripartum decidua and identified some key intercellular communications involved in labor and maternal-fetal immune tolerance. We believe that our study provides valuable clues for understanding the mechanisms of pregnancy and provides possible diagnostic strategies for the onset of labor.
format	article
author	Jingrui Huang Weishe Zhang Weishe Zhang Yanhua Zhao Jingzhi Li Mingkun Xie Yang Lu Qiaozhen Peng Jiejie Zhang Ping Li Lei Dai Lei Dai
author_facet	Jingrui Huang Weishe Zhang Weishe Zhang Yanhua Zhao Jingzhi Li Mingkun Xie Yang Lu Qiaozhen Peng Jiejie Zhang Ping Li Lei Dai Lei Dai
author_sort	Jingrui Huang
title	Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
title_short	Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
title_full	Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
title_fullStr	Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
title_full_unstemmed	Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery
title_sort	deciphering the intercellular communication network of peripartum decidua that orchestrates delivery
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/667eb6ff40fc413a8c22d48e3b436755
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Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

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