Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)

Abstract Identification of markers associated with major physiological and yield component traits under moisture deficit stress conditions in preferred donor lines paves the way for marker-assisted selection (MAS). In the present study, a set of 183 backcross inbred lines (BILs) derived from the cro...

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Autores principales:	Shashikumara Puttamadanayaka, Harikrishna, Manu Balaramaiah, Sunil Biradar, Sunilkumar V. Parmeshwarappa, Nivedita Sinha, S. V. Sai Prasad, P. C. Mishra, Neelu Jain, Pradeep Kumar Singh, Gyanendra Pratap Singh, Kumble Vinod Prabhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6685360e6cc046fba7d7e969ea69bb11
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spelling	oai:doaj.org-article:6685360e6cc046fba7d7e969ea69bb112021-12-02T12:33:15ZMapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)10.1038/s41598-020-78671-x2045-2322https://doaj.org/article/6685360e6cc046fba7d7e969ea69bb112020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78671-xhttps://doaj.org/toc/2045-2322Abstract Identification of markers associated with major physiological and yield component traits under moisture deficit stress conditions in preferred donor lines paves the way for marker-assisted selection (MAS). In the present study, a set of 183 backcross inbred lines (BILs) derived from the cross HD2733/2*C306 were genotyped using 35K Axiom genotyping array and SSR markers. The multi-trait, multi-location field phenotyping of BILs was done at three locations covering two major wheat growing zones of India, north-western plains zone (NWPZ) and central zone (CZ) under varying moisture regimes. A linkage map was constructed using 705 SNPs and 86 SSR polymorphic markers. A total of 43 genomic regions and QTL × QTL epistatic interactions were identified for 14 physiological and yield component traits, including NDVI, chlorophyll content, CT, CL, PH, GWPS, TGW and GY. Chromosomes 2A, 5D, 5A and 4B harbors greater number of QTLs for these traits. Seven Stable QTLs were identified across environment for DH (QDh.iari_6D), GWPS (QGWPS.iari_5B), PH (QPh.iari_4B-2, QPh.iari_4B-3) and NDVI (QNdvi1.iari_5D, QNdvi3.iari_5A). Nine genomic regions identified carrying major QTLs for CL, NDVI, RWC, FLA, PH, TGW and biomass explaining 10.32–28.35% of the phenotypic variance. The co-segregation of QTLs of physiological traits with yield component traits indicate the pleiotropic effects and their usefulness in the breeding programme. Our findings will be useful in dissecting genetic nature and marker-assisted selection for moisture deficit stress tolerance in wheat.Shashikumara PuttamadanayakaHarikrishnaManu BalaramaiahSunil BiradarSunilkumar V. ParmeshwarappaNivedita SinhaS. V. Sai PrasadP. C. MishraNeelu JainPradeep Kumar SinghGyanendra Pratap SinghKumble Vinod PrabhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shashikumara Puttamadanayaka Harikrishna Manu Balaramaiah Sunil Biradar Sunilkumar V. Parmeshwarappa Nivedita Sinha S. V. Sai Prasad P. C. Mishra Neelu Jain Pradeep Kumar Singh Gyanendra Pratap Singh Kumble Vinod Prabhu Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
description	Abstract Identification of markers associated with major physiological and yield component traits under moisture deficit stress conditions in preferred donor lines paves the way for marker-assisted selection (MAS). In the present study, a set of 183 backcross inbred lines (BILs) derived from the cross HD2733/2*C306 were genotyped using 35K Axiom genotyping array and SSR markers. The multi-trait, multi-location field phenotyping of BILs was done at three locations covering two major wheat growing zones of India, north-western plains zone (NWPZ) and central zone (CZ) under varying moisture regimes. A linkage map was constructed using 705 SNPs and 86 SSR polymorphic markers. A total of 43 genomic regions and QTL × QTL epistatic interactions were identified for 14 physiological and yield component traits, including NDVI, chlorophyll content, CT, CL, PH, GWPS, TGW and GY. Chromosomes 2A, 5D, 5A and 4B harbors greater number of QTLs for these traits. Seven Stable QTLs were identified across environment for DH (QDh.iari_6D), GWPS (QGWPS.iari_5B), PH (QPh.iari_4B-2, QPh.iari_4B-3) and NDVI (QNdvi1.iari_5D, QNdvi3.iari_5A). Nine genomic regions identified carrying major QTLs for CL, NDVI, RWC, FLA, PH, TGW and biomass explaining 10.32–28.35% of the phenotypic variance. The co-segregation of QTLs of physiological traits with yield component traits indicate the pleiotropic effects and their usefulness in the breeding programme. Our findings will be useful in dissecting genetic nature and marker-assisted selection for moisture deficit stress tolerance in wheat.
format	article
author	Shashikumara Puttamadanayaka Harikrishna Manu Balaramaiah Sunil Biradar Sunilkumar V. Parmeshwarappa Nivedita Sinha S. V. Sai Prasad P. C. Mishra Neelu Jain Pradeep Kumar Singh Gyanendra Pratap Singh Kumble Vinod Prabhu
author_facet	Shashikumara Puttamadanayaka Harikrishna Manu Balaramaiah Sunil Biradar Sunilkumar V. Parmeshwarappa Nivedita Sinha S. V. Sai Prasad P. C. Mishra Neelu Jain Pradeep Kumar Singh Gyanendra Pratap Singh Kumble Vinod Prabhu
author_sort	Shashikumara Puttamadanayaka
title	Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
title_short	Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
title_full	Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
title_fullStr	Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
title_full_unstemmed	Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)
title_sort	mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (triticum aestivum l.)
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6685360e6cc046fba7d7e969ea69bb11
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Mapping genomic regions of moisture deficit stress tolerance using backcross inbred lines in wheat (Triticum aestivum L.)

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