Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium

AIM: To characterize the anti-inflammatory and anti-apoptotic effects of N-acetylcysteine (NAC) in streptozotocin (STZ)-induced diabetic rat corneal epithelium and human corneal epithelial cells (HCECs) exposed to a high-glucose environment. METHODS: HCECs were incubated in 0, 5, 50 mmol/L glucose m...

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Autores principales:	Sae-Byeok Hwang, Jin Hyoung Park, Ji-Yun Park, Soon-Suk Kang, Ho Seok Chung, Hun Lee, Jae Yong Kim, Hungwon Tchah
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	n-acetylcysteine apoptosis inflammation diabetes corneal epithelium rat Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/66875ae847054e169558252a892fc915
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spelling	oai:doaj.org-article:66875ae847054e169558252a892fc9152021-11-26T04:02:12ZAnti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium2222-39592227-489810.18240/ijo.2021.12.01https://doaj.org/article/66875ae847054e169558252a892fc9152021-12-01T00:00:00Zhttp://ies.ijo.cn/en_publish/2021/12/20211201.pdfhttps://doaj.org/toc/2222-3959https://doaj.org/toc/2227-4898AIM: To characterize the anti-inflammatory and anti-apoptotic effects of N-acetylcysteine (NAC) in streptozotocin (STZ)-induced diabetic rat corneal epithelium and human corneal epithelial cells (HCECs) exposed to a high-glucose environment. METHODS: HCECs were incubated in 0, 5, 50 mmol/L glucose medium, or 50 mmol/L glucose medium with NAC for 24h. Diabetes was induced in rats by intraperitoneal injection of 65 mg/kg STZ and some of these rats were topically administered NAC to corneas with 3 mice per group. We characterized receptor for advanced glycation end-products (RAGE) expression using immunofluorescence, and interleukin (IL)-1β and cleaved caspase-3 (CCAP-3) expression using immunohistochemistry. Circulating tumor necrosis factor (TNF)-α concentration was measured by ELISA and cleaved poly-ADP ribose polymerase (PARP) concentration was quantified by Western blotting. Apoptotic cells were detected using TUNEL assay and annexin V and propidium iodide staining. RESULTS: Diabetic rats had higher expression of RAGE (2.46±0.13 fold), IL-1β, and CCAP-3 in apoptotic cells of their corneas than control rats. The expression of RAGE (1.83±0.11 fold), IL-1β, and CCAP-3, and the number of apoptotic cells, were reduced by topical NAC treatment. HCECs incubated in 50 mmol/L glucose medium showed high concentrations of TNF-α (310±2.00 pg/mL) and cleaved PARP (7.43±0.56 fold), and more extensive apoptosis than cells in 50 mmol/L glucose medium. However, the addition of NAC reduced the concentrations of TNF-α (153.67±2.31 pg/mL) and cleaved PARP (5.55±0.31 fold) and the number of apoptotic cells. CONCLUSION: NAC inhibits inflammation and apoptosis in the corneas of diabetic rats and HCECs maintained in a high-glucose environment.Sae-Byeok HwangJin Hyoung ParkJi-Yun ParkSoon-Suk KangHo Seok ChungHun LeeJae Yong KimHungwon TchahPress of International Journal of Ophthalmology (IJO PRESS)articlen-acetylcysteineapoptosisinflammationdiabetescorneal epitheliumratOphthalmologyRE1-994ENInternational Journal of Ophthalmology, Vol 14, Iss 12, Pp 1805-1812 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	n-acetylcysteine apoptosis inflammation diabetes corneal epithelium rat Ophthalmology RE1-994
spellingShingle	n-acetylcysteine apoptosis inflammation diabetes corneal epithelium rat Ophthalmology RE1-994 Sae-Byeok Hwang Jin Hyoung Park Ji-Yun Park Soon-Suk Kang Ho Seok Chung Hun Lee Jae Yong Kim Hungwon Tchah Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
description	AIM: To characterize the anti-inflammatory and anti-apoptotic effects of N-acetylcysteine (NAC) in streptozotocin (STZ)-induced diabetic rat corneal epithelium and human corneal epithelial cells (HCECs) exposed to a high-glucose environment. METHODS: HCECs were incubated in 0, 5, 50 mmol/L glucose medium, or 50 mmol/L glucose medium with NAC for 24h. Diabetes was induced in rats by intraperitoneal injection of 65 mg/kg STZ and some of these rats were topically administered NAC to corneas with 3 mice per group. We characterized receptor for advanced glycation end-products (RAGE) expression using immunofluorescence, and interleukin (IL)-1β and cleaved caspase-3 (CCAP-3) expression using immunohistochemistry. Circulating tumor necrosis factor (TNF)-α concentration was measured by ELISA and cleaved poly-ADP ribose polymerase (PARP) concentration was quantified by Western blotting. Apoptotic cells were detected using TUNEL assay and annexin V and propidium iodide staining. RESULTS: Diabetic rats had higher expression of RAGE (2.46±0.13 fold), IL-1β, and CCAP-3 in apoptotic cells of their corneas than control rats. The expression of RAGE (1.83±0.11 fold), IL-1β, and CCAP-3, and the number of apoptotic cells, were reduced by topical NAC treatment. HCECs incubated in 50 mmol/L glucose medium showed high concentrations of TNF-α (310±2.00 pg/mL) and cleaved PARP (7.43±0.56 fold), and more extensive apoptosis than cells in 50 mmol/L glucose medium. However, the addition of NAC reduced the concentrations of TNF-α (153.67±2.31 pg/mL) and cleaved PARP (5.55±0.31 fold) and the number of apoptotic cells. CONCLUSION: NAC inhibits inflammation and apoptosis in the corneas of diabetic rats and HCECs maintained in a high-glucose environment.
format	article
author	Sae-Byeok Hwang Jin Hyoung Park Ji-Yun Park Soon-Suk Kang Ho Seok Chung Hun Lee Jae Yong Kim Hungwon Tchah
author_facet	Sae-Byeok Hwang Jin Hyoung Park Ji-Yun Park Soon-Suk Kang Ho Seok Chung Hun Lee Jae Yong Kim Hungwon Tchah
author_sort	Sae-Byeok Hwang
title	Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
title_short	Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
title_full	Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
title_fullStr	Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
title_full_unstemmed	Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium
title_sort	anti-inflammatory and anti-apoptotic effects of n-acetylcysteine in diabetic rat corneal epithelium
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/66875ae847054e169558252a892fc915
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_version_	1718409905666260992

Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium

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