Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure

Abstract During the last decades discussions were taking place on the existence of global, non-thermal structural changes in biological macromolecules induced by Terahertz (THz) radiation. Despite numerous studies, a clear experimental proof of this effect for biological particles in solution is sti...

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Autores principales:	Martin A. Schroer, Siawosch Schewa, Andrey Yu. Gruzinov, Christian Rönnau, Janine Mia Lahey-Rudolph, Clement E. Blanchet, Till Zickmantel, Young-Hwa Song, Dmitri I. Svergun, Manfred Roessle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/668ea2ef468f4b189896403cd4d47cbb
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spelling	oai:doaj.org-article:668ea2ef468f4b189896403cd4d47cbb2021-11-21T12:20:10ZProbing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure10.1038/s41598-021-01774-62045-2322https://doaj.org/article/668ea2ef468f4b189896403cd4d47cbb2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01774-6https://doaj.org/toc/2045-2322Abstract During the last decades discussions were taking place on the existence of global, non-thermal structural changes in biological macromolecules induced by Terahertz (THz) radiation. Despite numerous studies, a clear experimental proof of this effect for biological particles in solution is still missing. We developed a setup combining THz-irradiation with small angle X-ray scattering (SAXS), which is a sensitive method for detecting the expected structural changes. We investigated in detail protein systems with different shape morphologies (bovine serum albumin, microtubules), which have been proposed to be susceptible to THz-radiation, under variable parameters (THz wavelength, THz power densities up to 6.8 mW/cm2, protein concentrations). None of the studied systems and conditions revealed structural changes detectable by SAXS suggesting that the expected non-thermal THz-induced effects do not lead to alterations of the overall structures, which are revealed by scattering from dissolved macromolecules. This leaves us with the conclusion that, if such effects are present, these are either local or outside of the spectrum and power range covered by the present study.Martin A. SchroerSiawosch SchewaAndrey Yu. GruzinovChristian RönnauJanine Mia Lahey-RudolphClement E. BlanchetTill ZickmantelYoung-Hwa SongDmitri I. SvergunManfred RoessleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin A. Schroer Siawosch Schewa Andrey Yu. Gruzinov Christian Rönnau Janine Mia Lahey-Rudolph Clement E. Blanchet Till Zickmantel Young-Hwa Song Dmitri I. Svergun Manfred Roessle Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
description	Abstract During the last decades discussions were taking place on the existence of global, non-thermal structural changes in biological macromolecules induced by Terahertz (THz) radiation. Despite numerous studies, a clear experimental proof of this effect for biological particles in solution is still missing. We developed a setup combining THz-irradiation with small angle X-ray scattering (SAXS), which is a sensitive method for detecting the expected structural changes. We investigated in detail protein systems with different shape morphologies (bovine serum albumin, microtubules), which have been proposed to be susceptible to THz-radiation, under variable parameters (THz wavelength, THz power densities up to 6.8 mW/cm2, protein concentrations). None of the studied systems and conditions revealed structural changes detectable by SAXS suggesting that the expected non-thermal THz-induced effects do not lead to alterations of the overall structures, which are revealed by scattering from dissolved macromolecules. This leaves us with the conclusion that, if such effects are present, these are either local or outside of the spectrum and power range covered by the present study.
format	article
author	Martin A. Schroer Siawosch Schewa Andrey Yu. Gruzinov Christian Rönnau Janine Mia Lahey-Rudolph Clement E. Blanchet Till Zickmantel Young-Hwa Song Dmitri I. Svergun Manfred Roessle
author_facet	Martin A. Schroer Siawosch Schewa Andrey Yu. Gruzinov Christian Rönnau Janine Mia Lahey-Rudolph Clement E. Blanchet Till Zickmantel Young-Hwa Song Dmitri I. Svergun Manfred Roessle
author_sort	Martin A. Schroer
title	Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
title_short	Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
title_full	Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
title_fullStr	Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
title_full_unstemmed	Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure
title_sort	probing the existence of non-thermal terahertz radiation induced changes of the protein solution structure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/668ea2ef468f4b189896403cd4d47cbb
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Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure

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