A polymer/oil based nanovaccine as a single-dose immunization approach.

The recognized necessity for new antigen delivery carriers with the capacity to boost, modulate and prolong neutralizing immune responses prompted our approach, in which we describe a multifunctional nanocarrier consisting of an oily nanocontainer protected by a polymeric shell made of chitosan (CS)...

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Autores principales:	Sara Vicente, Belen Diaz-Freitas, Mercedes Peleteiro, Alejandro Sanchez, David W Pascual, Africa Gonzalez-Fernandez, Maria J Alonso
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/669d2fc157904a95ad29517040c238dd
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spelling	oai:doaj.org-article:669d2fc157904a95ad29517040c238dd2021-11-18T07:48:36ZA polymer/oil based nanovaccine as a single-dose immunization approach.1932-620310.1371/journal.pone.0062500https://doaj.org/article/669d2fc157904a95ad29517040c238dd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23614052/?tool=EBIhttps://doaj.org/toc/1932-6203The recognized necessity for new antigen delivery carriers with the capacity to boost, modulate and prolong neutralizing immune responses prompted our approach, in which we describe a multifunctional nanocarrier consisting of an oily nanocontainer protected by a polymeric shell made of chitosan (CS), named CS nanocapsules (CSNC). The CS shell can associate the antigen on its surface, whereas the oily core might provide additional immunostimulating properties. In this first characterization of the system, we intended to study the influence of different antigen organizations on the nanocarrier's surface (using the recombinant hepatitis B surface antigen -rHBsAg- as a model antigen) on their long-term immunopotentiating effect, without any additional immunostimulant. Thus, two prototypes of antigen-loaded CSNC (CSNC+ and CSNC-), exhibiting similar particle size (200 nm) and high antigen association efficiency (>80%), were developed with different surface composition (polymer/antigen ratios) and surface charge (positive/negative, respectively). The biological evaluation of these nanovaccines evidenced the superiority of the CSNC+ as compared to CSNC- and alum-rHBsAg in terms of neutralizing antibody responses, following intramuscular vaccination. Moreover, a single dose of CSNC+ led to similar IgG levels to the positive control. The IgG1/IgG2a ratio suggested a mixed Th1/Th2 response elicited by CSNC+, in contrast to the typical Th2-biased response of alum. Finally, CSNC+ could be freeze-dried without altering its physicochemical properties and adjuvant effect in vivo. In conclusion, the evaluation of CSNC+ confirms its interesting features for enhancing, prolonging and modulating the type of immune response against subunit antigens, such as rHBsAg.Sara VicenteBelen Diaz-FreitasMercedes PeleteiroAlejandro SanchezDavid W PascualAfrica Gonzalez-FernandezMaria J AlonsoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62500 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Vicente Belen Diaz-Freitas Mercedes Peleteiro Alejandro Sanchez David W Pascual Africa Gonzalez-Fernandez Maria J Alonso A polymer/oil based nanovaccine as a single-dose immunization approach.
description	The recognized necessity for new antigen delivery carriers with the capacity to boost, modulate and prolong neutralizing immune responses prompted our approach, in which we describe a multifunctional nanocarrier consisting of an oily nanocontainer protected by a polymeric shell made of chitosan (CS), named CS nanocapsules (CSNC). The CS shell can associate the antigen on its surface, whereas the oily core might provide additional immunostimulating properties. In this first characterization of the system, we intended to study the influence of different antigen organizations on the nanocarrier's surface (using the recombinant hepatitis B surface antigen -rHBsAg- as a model antigen) on their long-term immunopotentiating effect, without any additional immunostimulant. Thus, two prototypes of antigen-loaded CSNC (CSNC+ and CSNC-), exhibiting similar particle size (200 nm) and high antigen association efficiency (>80%), were developed with different surface composition (polymer/antigen ratios) and surface charge (positive/negative, respectively). The biological evaluation of these nanovaccines evidenced the superiority of the CSNC+ as compared to CSNC- and alum-rHBsAg in terms of neutralizing antibody responses, following intramuscular vaccination. Moreover, a single dose of CSNC+ led to similar IgG levels to the positive control. The IgG1/IgG2a ratio suggested a mixed Th1/Th2 response elicited by CSNC+, in contrast to the typical Th2-biased response of alum. Finally, CSNC+ could be freeze-dried without altering its physicochemical properties and adjuvant effect in vivo. In conclusion, the evaluation of CSNC+ confirms its interesting features for enhancing, prolonging and modulating the type of immune response against subunit antigens, such as rHBsAg.
format	article
author	Sara Vicente Belen Diaz-Freitas Mercedes Peleteiro Alejandro Sanchez David W Pascual Africa Gonzalez-Fernandez Maria J Alonso
author_facet	Sara Vicente Belen Diaz-Freitas Mercedes Peleteiro Alejandro Sanchez David W Pascual Africa Gonzalez-Fernandez Maria J Alonso
author_sort	Sara Vicente
title	A polymer/oil based nanovaccine as a single-dose immunization approach.
title_short	A polymer/oil based nanovaccine as a single-dose immunization approach.
title_full	A polymer/oil based nanovaccine as a single-dose immunization approach.
title_fullStr	A polymer/oil based nanovaccine as a single-dose immunization approach.
title_full_unstemmed	A polymer/oil based nanovaccine as a single-dose immunization approach.
title_sort	polymer/oil based nanovaccine as a single-dose immunization approach.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/669d2fc157904a95ad29517040c238dd
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A polymer/oil based nanovaccine as a single-dose immunization approach.

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