Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis

Analysis, purification, and characterization of AFB1 degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB1...

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Autores principales:	Yan-Duo Wang, Cheng-Gang Song, Jian Yang, Tao Zhou, Yu-Yang Zhao, Jian-Chun Qin, Lan-Ping Guo, Gang Ding
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	aflatoxin B1 UPLC-Q-TOF-MS/MS degraded products purification NMR Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/669ddbef7cc349abb9cb7f3b7d9f0e98
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spelling	oai:doaj.org-article:669ddbef7cc349abb9cb7f3b7d9f0e982021-11-30T18:27:25ZAccurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis2296-264610.3389/fchem.2021.789249https://doaj.org/article/669ddbef7cc349abb9cb7f3b7d9f0e982021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.789249/fullhttps://doaj.org/toc/2296-2646Analysis, purification, and characterization of AFB1 degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB1 photocatalyzed under UV irradiation. A total of seventeen degraded products were characterized based on the UPLC-Q-TOF-MS/MS analysis, in which seven ones (1–7) including four (stereo) isomers (1,2, 5, and 6) were purified and elucidated by NMR experiments. According to the structural features of AFB1 and degraded products (1–7), the possible photocatalytic mechanisms were suggested. Furthermore, AFB1 and degraded products (1–7) were evaluated against different cell lines. The results indicated that the UPLC-Q-TOF-MS/MS technique combined with purification, NMR spectral experiments, and biological tests was an applicable integrated approach for analysis, characterization, and toxic evaluation of degraded products of AFB1, which could be used to evaluate other mycotoxin degradation processes.Yan-Duo WangCheng-Gang SongJian YangTao ZhouYu-Yang ZhaoJian-Chun QinLan-Ping GuoGang DingFrontiers Media S.A.articleaflatoxin B1UPLC-Q-TOF-MS/MSdegraded productspurificationNMRChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aflatoxin B1 UPLC-Q-TOF-MS/MS degraded products purification NMR Chemistry QD1-999
spellingShingle	aflatoxin B1 UPLC-Q-TOF-MS/MS degraded products purification NMR Chemistry QD1-999 Yan-Duo Wang Cheng-Gang Song Jian Yang Tao Zhou Yu-Yang Zhao Jian-Chun Qin Lan-Ping Guo Gang Ding Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
description	Analysis, purification, and characterization of AFB1 degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB1 photocatalyzed under UV irradiation. A total of seventeen degraded products were characterized based on the UPLC-Q-TOF-MS/MS analysis, in which seven ones (1–7) including four (stereo) isomers (1,2, 5, and 6) were purified and elucidated by NMR experiments. According to the structural features of AFB1 and degraded products (1–7), the possible photocatalytic mechanisms were suggested. Furthermore, AFB1 and degraded products (1–7) were evaluated against different cell lines. The results indicated that the UPLC-Q-TOF-MS/MS technique combined with purification, NMR spectral experiments, and biological tests was an applicable integrated approach for analysis, characterization, and toxic evaluation of degraded products of AFB1, which could be used to evaluate other mycotoxin degradation processes.
format	article
author	Yan-Duo Wang Cheng-Gang Song Jian Yang Tao Zhou Yu-Yang Zhao Jian-Chun Qin Lan-Ping Guo Gang Ding
author_facet	Yan-Duo Wang Cheng-Gang Song Jian Yang Tao Zhou Yu-Yang Zhao Jian-Chun Qin Lan-Ping Guo Gang Ding
author_sort	Yan-Duo Wang
title	Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
title_short	Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
title_full	Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
title_fullStr	Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
title_full_unstemmed	Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
title_sort	accurate identification of degraded products of aflatoxin b1 under uv irradiation based on uplc-q-tof-ms/ms and nmr analysis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/669ddbef7cc349abb9cb7f3b7d9f0e98
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Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis

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