Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp

Abstract Several factors, including bacterial and viral infections, have been associated with rhinosinusitis and nasal tissue remodelling that may result in nasal polyp formation. However, the potential role of bacterial or viral stimuli triggering polyp development is unclear. Here, we used lipopol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jee Hye Wee, Young-Kyung Ko, Roza Khalmuratova, Hyun-Woo Shin, Dae Woo Kim, Chae-Seo Rhee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66a1662d8cbc4387a2fc2aa98743ed17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:66a1662d8cbc4387a2fc2aa98743ed17
record_format	dspace
spelling	oai:doaj.org-article:66a1662d8cbc4387a2fc2aa98743ed172021-12-02T14:12:42ZEffect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp10.1038/s41598-020-80483-y2045-2322https://doaj.org/article/66a1662d8cbc4387a2fc2aa98743ed172021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80483-yhttps://doaj.org/toc/2045-2322Abstract Several factors, including bacterial and viral infections, have been associated with rhinosinusitis and nasal tissue remodelling that may result in nasal polyp formation. However, the potential role of bacterial or viral stimuli triggering polyp development is unclear. Here, we used lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [poly(I:C)] in a murine model of allergic rhinosinusitis to compare different effects of bacterial- and virus-derived stimuli in the pathogenesis of nasal polyp formation. Briefly, BALB/c mice were sensitised and challenged with ovalbumin and staphylococcal enterotoxin, with or without LPS or poly(I:C), and the consequent histopathological profiles, cytokines, and systemic humoral responses were studied. While no significant differences in polyp formations and epithelial disruptions were observed among the experimental groups, the local cell recruitment patterns slightly differed in animals that received either LPS or poly(I:C). Additionally, the local immune environments generated by LPS or poly(I:C) stimulation varied. LPS stimulation induced a marked Th1/Th17 response and predominantly neutrophilic nasal polyp formations, whereas poly(I:C) induced a Th2-skewed environment in neutrophilic nasal polyp development. Overall, our findings show that both cell recruitment patterns and local immune environments induced by these two stimuli differ, which may have implications in the physiopathology of rhinosinusitis with nasal polyp.Jee Hye WeeYoung-Kyung KoRoza KhalmuratovaHyun-Woo ShinDae Woo KimChae-Seo RheeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jee Hye Wee Young-Kyung Ko Roza Khalmuratova Hyun-Woo Shin Dae Woo Kim Chae-Seo Rhee Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
description	Abstract Several factors, including bacterial and viral infections, have been associated with rhinosinusitis and nasal tissue remodelling that may result in nasal polyp formation. However, the potential role of bacterial or viral stimuli triggering polyp development is unclear. Here, we used lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [poly(I:C)] in a murine model of allergic rhinosinusitis to compare different effects of bacterial- and virus-derived stimuli in the pathogenesis of nasal polyp formation. Briefly, BALB/c mice were sensitised and challenged with ovalbumin and staphylococcal enterotoxin, with or without LPS or poly(I:C), and the consequent histopathological profiles, cytokines, and systemic humoral responses were studied. While no significant differences in polyp formations and epithelial disruptions were observed among the experimental groups, the local cell recruitment patterns slightly differed in animals that received either LPS or poly(I:C). Additionally, the local immune environments generated by LPS or poly(I:C) stimulation varied. LPS stimulation induced a marked Th1/Th17 response and predominantly neutrophilic nasal polyp formations, whereas poly(I:C) induced a Th2-skewed environment in neutrophilic nasal polyp development. Overall, our findings show that both cell recruitment patterns and local immune environments induced by these two stimuli differ, which may have implications in the physiopathology of rhinosinusitis with nasal polyp.
format	article
author	Jee Hye Wee Young-Kyung Ko Roza Khalmuratova Hyun-Woo Shin Dae Woo Kim Chae-Seo Rhee
author_facet	Jee Hye Wee Young-Kyung Ko Roza Khalmuratova Hyun-Woo Shin Dae Woo Kim Chae-Seo Rhee
author_sort	Jee Hye Wee
title	Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
title_short	Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
title_full	Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
title_fullStr	Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
title_full_unstemmed	Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
title_sort	effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66a1662d8cbc4387a2fc2aa98743ed17
work_keys_str_mv	AT jeehyewee effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp AT youngkyungko effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp AT rozakhalmuratova effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp AT hyunwooshin effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp AT daewookim effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp AT chaeseorhee effectoflipopolysaccharideandpolyinosinicpolycytidylicacidinamurinemodelofnasalpolyp
_version_	1718391787291148288

Effect of lipopolysaccharide and polyinosinic:polycytidylic acid in a murine model of nasal polyp

Ejemplares similares