Transcriptional analysis of the response of C. elegans to ethanol exposure

Abstract Ethanol-induced transcriptional changes underlie important physiological responses to ethanol that are likely to contribute to the addictive properties of the drug. We examined the transcriptional responses of Caenorhabditis elegans across a timecourse of ethanol exposure, between 30 min an...

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Autores principales:	Mark G. Sterken, Marijke H. van Wijk, Elizabeth C. Quamme, Joost A. G. Riksen, Lucinda Carnell, Laura D. Mathies, Andrew G. Davies, Jan E. Kammenga, Jill C. Bettinger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66a7b91017484a01971b473c2c84f1bc
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spelling	oai:doaj.org-article:66a7b91017484a01971b473c2c84f1bc2021-12-02T14:49:12ZTranscriptional analysis of the response of C. elegans to ethanol exposure10.1038/s41598-021-90282-82045-2322https://doaj.org/article/66a7b91017484a01971b473c2c84f1bc2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90282-8https://doaj.org/toc/2045-2322Abstract Ethanol-induced transcriptional changes underlie important physiological responses to ethanol that are likely to contribute to the addictive properties of the drug. We examined the transcriptional responses of Caenorhabditis elegans across a timecourse of ethanol exposure, between 30 min and 8 h, to determine what genes and genetic pathways are regulated in response to ethanol in this model. We found that short exposures to ethanol (up to 2 h) induced expression of metabolic enzymes involved in metabolizing ethanol and retinol, while longer exposure (8 h) had much more profound effects on the transcriptome. Several genes that are known to be involved in the physiological response to ethanol, including direct ethanol targets, were regulated at 8 h of exposure. This longer exposure to ethanol also resulted in the regulation of genes involved in cilia function, which is consistent with an important role for the effects of ethanol on cilia in the deleterious effects of chronic ethanol consumption in humans. Finally, we found that food deprivation for an 8-h period induced gene expression changes that were somewhat ameliorated by the presence of ethanol, supporting previous observations that worms can use ethanol as a calorie source.Mark G. SterkenMarijke H. van WijkElizabeth C. QuammeJoost A. G. RiksenLucinda CarnellLaura D. MathiesAndrew G. DaviesJan E. KammengaJill C. BettingerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark G. Sterken Marijke H. van Wijk Elizabeth C. Quamme Joost A. G. Riksen Lucinda Carnell Laura D. Mathies Andrew G. Davies Jan E. Kammenga Jill C. Bettinger Transcriptional analysis of the response of C. elegans to ethanol exposure
description	Abstract Ethanol-induced transcriptional changes underlie important physiological responses to ethanol that are likely to contribute to the addictive properties of the drug. We examined the transcriptional responses of Caenorhabditis elegans across a timecourse of ethanol exposure, between 30 min and 8 h, to determine what genes and genetic pathways are regulated in response to ethanol in this model. We found that short exposures to ethanol (up to 2 h) induced expression of metabolic enzymes involved in metabolizing ethanol and retinol, while longer exposure (8 h) had much more profound effects on the transcriptome. Several genes that are known to be involved in the physiological response to ethanol, including direct ethanol targets, were regulated at 8 h of exposure. This longer exposure to ethanol also resulted in the regulation of genes involved in cilia function, which is consistent with an important role for the effects of ethanol on cilia in the deleterious effects of chronic ethanol consumption in humans. Finally, we found that food deprivation for an 8-h period induced gene expression changes that were somewhat ameliorated by the presence of ethanol, supporting previous observations that worms can use ethanol as a calorie source.
format	article
author	Mark G. Sterken Marijke H. van Wijk Elizabeth C. Quamme Joost A. G. Riksen Lucinda Carnell Laura D. Mathies Andrew G. Davies Jan E. Kammenga Jill C. Bettinger
author_facet	Mark G. Sterken Marijke H. van Wijk Elizabeth C. Quamme Joost A. G. Riksen Lucinda Carnell Laura D. Mathies Andrew G. Davies Jan E. Kammenga Jill C. Bettinger
author_sort	Mark G. Sterken
title	Transcriptional analysis of the response of C. elegans to ethanol exposure
title_short	Transcriptional analysis of the response of C. elegans to ethanol exposure
title_full	Transcriptional analysis of the response of C. elegans to ethanol exposure
title_fullStr	Transcriptional analysis of the response of C. elegans to ethanol exposure
title_full_unstemmed	Transcriptional analysis of the response of C. elegans to ethanol exposure
title_sort	transcriptional analysis of the response of c. elegans to ethanol exposure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66a7b91017484a01971b473c2c84f1bc
work_keys_str_mv	AT markgsterken transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT marijkehvanwijk transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT elizabethcquamme transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT joostagriksen transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT lucindacarnell transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT lauradmathies transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT andrewgdavies transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT janekammenga transcriptionalanalysisoftheresponseofceleganstoethanolexposure AT jillcbettinger transcriptionalanalysisoftheresponseofceleganstoethanolexposure
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Transcriptional analysis of the response of C. elegans to ethanol exposure

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