Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.

<h4>Background</h4>The Kato-Katz is the most common diagnostic method for Schistosoma mansoni infection. However, the day-to-day variability in host egg-excretion and its low detection sensitivity are major limits for its use in low transmission zones and after widespread chemotherapy. W...

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Autores principales:	Louis-Albert Tchuem Tchuenté, Césaire Joris Kueté Fouodo, Romuald Isaka Kamwa Ngassam, Laurentine Sumo, Calvine Dongmo Noumedem, Christian Mérimé Kenfack, Nestor Feussom Gipwe, Esther Dankoni Nana, J Russell Stothard, David Rollinson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/66a80b6b10ca48a0a0373157d6b3e48f
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spelling	oai:doaj.org-article:66a80b6b10ca48a0a0373157d6b3e48f2021-11-18T09:13:57ZEvaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.1935-27271935-273510.1371/journal.pntd.0001758https://doaj.org/article/66a80b6b10ca48a0a0373157d6b3e48f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22860148/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>The Kato-Katz is the most common diagnostic method for Schistosoma mansoni infection. However, the day-to-day variability in host egg-excretion and its low detection sensitivity are major limits for its use in low transmission zones and after widespread chemotherapy. We evaluated the accuracy of circulating cathodic antigen (CCA) urine-assay as a diagnostic tool of S. mansoni. In comparison, a low sensitive CCA test (CCA-L) was assessed.<h4>Methodology</h4>THE STUDY WAS CONDUCTED IN THREE SETTINGS: two foci with single S. mansoni infections (settings A and B), and one mixed S. mansoni - S. haematobium focus (setting C). Stool and urine samples were collected from school-children on three consecutive days. Triplicate Kato-Katz readings were performed per stool sample. Each urine sample was tested with one CCA and only the first urine sample was subjected to CCA-L. Urine samples were also examined for S. haematobium eggs using the filtration method and for microhaematuria using urine reagent strips. Overall, 625 children provided three stool and three urine samples.<h4>Principal findings</h4>Considering nine Kato-Katz thick smears as 'reference' diagnostic test, the prevalence of S. mansoni was 36.2%, 71.8% and 64.0% in settings A, B and C, respectively. The prevalence of S. haematobium in setting C was 12.0%. The sensitivities of single Kato-Katz, CCA and CCA-L from the first stool or urine samples were 58%, 82% and 46% in setting A, 56.8%, 82.4% and 68.8% in setting B, and 49.0%, 87.7% and 55.5% in setting C. The respective specificities were 100%, 64.7% and 100%; 100%, 62.3% and 91.3%; and 100%, 42.5% and 92.0%. Mixed infection with S. haematobium did not influence the CCA test results for S. mansoni diagnosis.<h4>Conclusions/significance</h4>Urine CCA revealed higher sensitivity than CCA-L and triplicate Kato-Katz, and produced similar prevalence as nine Kato-Katz. It seems an attractive method for S. mansoni diagnosis.Louis-Albert Tchuem TchuentéCésaire Joris Kueté FouodoRomuald Isaka Kamwa NgassamLaurentine SumoCalvine Dongmo NoumedemChristian Mérimé KenfackNestor Feussom GipweEsther Dankoni NanaJ Russell StothardDavid RollinsonPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 6, Iss 7, p e1758 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Louis-Albert Tchuem Tchuenté Césaire Joris Kueté Fouodo Romuald Isaka Kamwa Ngassam Laurentine Sumo Calvine Dongmo Noumedem Christian Mérimé Kenfack Nestor Feussom Gipwe Esther Dankoni Nana J Russell Stothard David Rollinson Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
description	<h4>Background</h4>The Kato-Katz is the most common diagnostic method for Schistosoma mansoni infection. However, the day-to-day variability in host egg-excretion and its low detection sensitivity are major limits for its use in low transmission zones and after widespread chemotherapy. We evaluated the accuracy of circulating cathodic antigen (CCA) urine-assay as a diagnostic tool of S. mansoni. In comparison, a low sensitive CCA test (CCA-L) was assessed.<h4>Methodology</h4>THE STUDY WAS CONDUCTED IN THREE SETTINGS: two foci with single S. mansoni infections (settings A and B), and one mixed S. mansoni - S. haematobium focus (setting C). Stool and urine samples were collected from school-children on three consecutive days. Triplicate Kato-Katz readings were performed per stool sample. Each urine sample was tested with one CCA and only the first urine sample was subjected to CCA-L. Urine samples were also examined for S. haematobium eggs using the filtration method and for microhaematuria using urine reagent strips. Overall, 625 children provided three stool and three urine samples.<h4>Principal findings</h4>Considering nine Kato-Katz thick smears as 'reference' diagnostic test, the prevalence of S. mansoni was 36.2%, 71.8% and 64.0% in settings A, B and C, respectively. The prevalence of S. haematobium in setting C was 12.0%. The sensitivities of single Kato-Katz, CCA and CCA-L from the first stool or urine samples were 58%, 82% and 46% in setting A, 56.8%, 82.4% and 68.8% in setting B, and 49.0%, 87.7% and 55.5% in setting C. The respective specificities were 100%, 64.7% and 100%; 100%, 62.3% and 91.3%; and 100%, 42.5% and 92.0%. Mixed infection with S. haematobium did not influence the CCA test results for S. mansoni diagnosis.<h4>Conclusions/significance</h4>Urine CCA revealed higher sensitivity than CCA-L and triplicate Kato-Katz, and produced similar prevalence as nine Kato-Katz. It seems an attractive method for S. mansoni diagnosis.
format	article
author	Louis-Albert Tchuem Tchuenté Césaire Joris Kueté Fouodo Romuald Isaka Kamwa Ngassam Laurentine Sumo Calvine Dongmo Noumedem Christian Mérimé Kenfack Nestor Feussom Gipwe Esther Dankoni Nana J Russell Stothard David Rollinson
author_facet	Louis-Albert Tchuem Tchuenté Césaire Joris Kueté Fouodo Romuald Isaka Kamwa Ngassam Laurentine Sumo Calvine Dongmo Noumedem Christian Mérimé Kenfack Nestor Feussom Gipwe Esther Dankoni Nana J Russell Stothard David Rollinson
author_sort	Louis-Albert Tchuem Tchuenté
title	Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
title_short	Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
title_full	Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
title_fullStr	Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
title_full_unstemmed	Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.
title_sort	evaluation of circulating cathodic antigen (cca) urine-tests for diagnosis of schistosoma mansoni infection in cameroon.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/66a80b6b10ca48a0a0373157d6b3e48f
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Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon.

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