Telomeric DNA sequences in beetle taxa vary with species richness

Abstract Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most commo...

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Autores principales:	Daniela Prušáková, Vratislav Peska, Stano Pekár, Michal Bubeník, Lukáš Čížek, Aleš Bezděk, Radmila Čapková Frydrychová
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66b01c3f6226475890c80dc43d07f29e
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spelling	oai:doaj.org-article:66b01c3f6226475890c80dc43d07f29e2021-12-02T16:07:04ZTelomeric DNA sequences in beetle taxa vary with species richness10.1038/s41598-021-92705-y2045-2322https://doaj.org/article/66b01c3f6226475890c80dc43d07f29e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92705-yhttps://doaj.org/toc/2045-2322Abstract Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern of telomeric sequences across eukaryotes is (TxAyGz)n maintained by telomerase. However, telomerase-added DNA repeats in some insect taxa frequently vary, show unusual features, and can even be absent. It has been speculated about factors that might allow frequent changes in telomere composition in Insecta. Coleoptera (beetles) is the largest of all insect orders and based on previously available data, it seemed that the telomeric sequence of beetles varies to a great extent. We performed an extensive mapping of the (TTAGG)n sequence, the ancestral telomeric sequence in Insects, across the main branches of Coleoptera. Our study indicates that the (TTAGG)n sequence has been repeatedly or completely lost in more than half of the tested beetle superfamilies. Although the exact telomeric motif in most of the (TTAGG)n-negative beetles is unknown, we found that the (TTAGG)n sequence has been replaced by two alternative telomeric motifs, the (TCAGG)n and (TTAGGG)n, in at least three superfamilies of Coleoptera. The diversity of the telomeric motifs was positively related to the species richness of taxa, regardless of the age of the taxa. The presence/absence of the (TTAGG)n sequence highly varied within the Curculionoidea, Chrysomeloidea, and Staphylinoidea, which are the three most diverse superfamilies within Metazoa. Our data supports the hypothesis that telomere dysfunctions can initiate rapid genomic changes that lead to reproductive isolation and speciation.Daniela PrušákováVratislav PeskaStano PekárMichal BubeníkLukáš ČížekAleš BezděkRadmila Čapková FrydrychováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Prušáková Vratislav Peska Stano Pekár Michal Bubeník Lukáš Čížek Aleš Bezděk Radmila Čapková Frydrychová Telomeric DNA sequences in beetle taxa vary with species richness
description	Abstract Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern of telomeric sequences across eukaryotes is (TxAyGz)n maintained by telomerase. However, telomerase-added DNA repeats in some insect taxa frequently vary, show unusual features, and can even be absent. It has been speculated about factors that might allow frequent changes in telomere composition in Insecta. Coleoptera (beetles) is the largest of all insect orders and based on previously available data, it seemed that the telomeric sequence of beetles varies to a great extent. We performed an extensive mapping of the (TTAGG)n sequence, the ancestral telomeric sequence in Insects, across the main branches of Coleoptera. Our study indicates that the (TTAGG)n sequence has been repeatedly or completely lost in more than half of the tested beetle superfamilies. Although the exact telomeric motif in most of the (TTAGG)n-negative beetles is unknown, we found that the (TTAGG)n sequence has been replaced by two alternative telomeric motifs, the (TCAGG)n and (TTAGGG)n, in at least three superfamilies of Coleoptera. The diversity of the telomeric motifs was positively related to the species richness of taxa, regardless of the age of the taxa. The presence/absence of the (TTAGG)n sequence highly varied within the Curculionoidea, Chrysomeloidea, and Staphylinoidea, which are the three most diverse superfamilies within Metazoa. Our data supports the hypothesis that telomere dysfunctions can initiate rapid genomic changes that lead to reproductive isolation and speciation.
format	article
author	Daniela Prušáková Vratislav Peska Stano Pekár Michal Bubeník Lukáš Čížek Aleš Bezděk Radmila Čapková Frydrychová
author_facet	Daniela Prušáková Vratislav Peska Stano Pekár Michal Bubeník Lukáš Čížek Aleš Bezděk Radmila Čapková Frydrychová
author_sort	Daniela Prušáková
title	Telomeric DNA sequences in beetle taxa vary with species richness
title_short	Telomeric DNA sequences in beetle taxa vary with species richness
title_full	Telomeric DNA sequences in beetle taxa vary with species richness
title_fullStr	Telomeric DNA sequences in beetle taxa vary with species richness
title_full_unstemmed	Telomeric DNA sequences in beetle taxa vary with species richness
title_sort	telomeric dna sequences in beetle taxa vary with species richness
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66b01c3f6226475890c80dc43d07f29e
work_keys_str_mv	AT danielaprusakova telomericdnasequencesinbeetletaxavarywithspeciesrichness AT vratislavpeska telomericdnasequencesinbeetletaxavarywithspeciesrichness AT stanopekar telomericdnasequencesinbeetletaxavarywithspeciesrichness AT michalbubenik telomericdnasequencesinbeetletaxavarywithspeciesrichness AT lukascizek telomericdnasequencesinbeetletaxavarywithspeciesrichness AT alesbezdek telomericdnasequencesinbeetletaxavarywithspeciesrichness AT radmilacapkovafrydrychova telomericdnasequencesinbeetletaxavarywithspeciesrichness
_version_	1718384779247747072

Telomeric DNA sequences in beetle taxa vary with species richness

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