Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes

New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Var...

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Autores principales:	Lucia Rosaria Forleo, Margherita D’Amico, Teodora Basile, Antonio Domenico Marsico, Maria Francesca Cardone, Flavia Angela Maria Maggiolini, Riccardo Velasco, Carlo Bergamini
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	table grapes genetic improvement new breeding techniques <i>Vitis vinifera</i> L. embryogenic callus Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/66b086443354488d928506ad9b14f389
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spelling	oai:doaj.org-article:66b086443354488d928506ad9b14f3892021-11-25T17:47:45ZSomatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes10.3390/horticulturae71105112311-7524https://doaj.org/article/66b086443354488d928506ad9b14f3892021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/511https://doaj.org/toc/2311-7524New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Varieties such as Italia have proven to be very recalcitrant to regeneration via somatic embryogenesis. In this work, the development of a protocol for improved production of embryogenic calluses is described. Two sterilization protocols were tested: (a) a lower active chlorine concentration for a longer time (LS); and (b) a higher chlorine concentration for a shorter time (HS), in combination with the absence or presence of citric acid in the growing substrate in the first growth media. The embryogenic calluses formation in Chardonnay, a cv. with a high embryogenic response, was significantly higher in presence of citric acid in the initial growing substrate regardless of the sterilization protocol. In Aglianico, a cv. with a lower embryogenic response, no significant differences were observed. Instead, in a recalcitrant cv. as Italia, we obtained a 13-fold increase in embryogenic calluses formation performing sterilization of flowers with the HS protocol compared to LS.Lucia Rosaria ForleoMargherita D’AmicoTeodora BasileAntonio Domenico MarsicoMaria Francesca CardoneFlavia Angela Maria MaggioliniRiccardo VelascoCarlo BergaminiMDPI AGarticletable grapesgenetic improvementnew breeding techniques<i>Vitis vinifera</i> L.embryogenic callusPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 511, p 511 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	table grapes genetic improvement new breeding techniques <i>Vitis vinifera</i> L. embryogenic callus Plant culture SB1-1110
spellingShingle	table grapes genetic improvement new breeding techniques <i>Vitis vinifera</i> L. embryogenic callus Plant culture SB1-1110 Lucia Rosaria Forleo Margherita D’Amico Teodora Basile Antonio Domenico Marsico Maria Francesca Cardone Flavia Angela Maria Maggiolini Riccardo Velasco Carlo Bergamini Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
description	New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Varieties such as Italia have proven to be very recalcitrant to regeneration via somatic embryogenesis. In this work, the development of a protocol for improved production of embryogenic calluses is described. Two sterilization protocols were tested: (a) a lower active chlorine concentration for a longer time (LS); and (b) a higher chlorine concentration for a shorter time (HS), in combination with the absence or presence of citric acid in the growing substrate in the first growth media. The embryogenic calluses formation in Chardonnay, a cv. with a high embryogenic response, was significantly higher in presence of citric acid in the initial growing substrate regardless of the sterilization protocol. In Aglianico, a cv. with a lower embryogenic response, no significant differences were observed. Instead, in a recalcitrant cv. as Italia, we obtained a 13-fold increase in embryogenic calluses formation performing sterilization of flowers with the HS protocol compared to LS.
format	article
author	Lucia Rosaria Forleo Margherita D’Amico Teodora Basile Antonio Domenico Marsico Maria Francesca Cardone Flavia Angela Maria Maggiolini Riccardo Velasco Carlo Bergamini
author_facet	Lucia Rosaria Forleo Margherita D’Amico Teodora Basile Antonio Domenico Marsico Maria Francesca Cardone Flavia Angela Maria Maggiolini Riccardo Velasco Carlo Bergamini
author_sort	Lucia Rosaria Forleo
title	Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
title_short	Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
title_full	Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
title_fullStr	Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
title_full_unstemmed	Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
title_sort	somatic embryogenesis in <i>vitis</i> for genome editing: optimization of protocols for recalcitrant genotypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/66b086443354488d928506ad9b14f389
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Somatic Embryogenesis in <i>Vitis</i> for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes

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