Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants

Abstract Programmed cell death (PCD) is one of the most intensively researched fields in modern mammalian biology with roles in cancer, aging, diabetes and numerous neurodegenerative diseases. It is becoming increasingly clear that PCD also plays significant roles in plant defence and responses to t...

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Autores principales:	Pauline Okemo, Hao Long, Yen Cheng, Sagadevan Mundree, Brett Williams
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66b5ca4e4b3a419998348edeb0d28bb4
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spelling	oai:doaj.org-article:66b5ca4e4b3a419998348edeb0d28bb42021-12-02T18:17:42ZStachyose triggers apoptotic like cell death in drought sensitive but not resilient plants10.1038/s41598-021-86559-72045-2322https://doaj.org/article/66b5ca4e4b3a419998348edeb0d28bb42021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86559-7https://doaj.org/toc/2045-2322Abstract Programmed cell death (PCD) is one of the most intensively researched fields in modern mammalian biology with roles in cancer, aging, diabetes and numerous neurodegenerative diseases. It is becoming increasingly clear that PCD also plays significant roles in plant defence and responses to the environment. Given their unique ability to tolerate desiccation (cells remain viable even after they’ve lost 95% of their water), resurrection plants make ideal models to study the regulation of plant PCD pathways. Previously, we showed that the Australian resurrection plant, Tripogon loliiformis, suppresses plant PCD, via trehalose-mediated activation of autophagy pathways, during drying. In the present study, we created a full-length T. loliiformis cDNA library, performed a large-scale Agrobacterium screen for improved salinity tolerance and identified Stachyose synthase (TlStach) as a potential candidate for improving stress tolerance. Tripogon loliiformis shoots accumulate stachyose synthase transcripts and stachyose during drying. Attempts to generate transgenic plants expressing TlStach failed and were consistent with previous reports in mammals that demonstrated stachyose-mediated induction of apoptosis. Using a combination of transcriptomics, metabolomics and cell death assays (TUNNEL and DNA laddering), we investigated whether stachyose induces apoptotic-like cell death in T. loliiformis. We show that stachyose triggers the formation of the hallmarks of plant apoptotic-like cell death in the desiccation sensitive Nicotiana benthamiana but not the resilient T. loliiformis. These findings suggest that T. loliiformis suppresses stachyose-mediated apoptotic-like cell death and provides insights on the role of sugar metabolism and plant PCD pathways. A better understanding of how resilient plants regulate sugar metabolism and PCD pathways may facilitate future targeting of plant metabolic pathways for increased stress tolerance.Pauline OkemoHao LongYen ChengSagadevan MundreeBrett WilliamsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pauline Okemo Hao Long Yen Cheng Sagadevan Mundree Brett Williams Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
description	Abstract Programmed cell death (PCD) is one of the most intensively researched fields in modern mammalian biology with roles in cancer, aging, diabetes and numerous neurodegenerative diseases. It is becoming increasingly clear that PCD also plays significant roles in plant defence and responses to the environment. Given their unique ability to tolerate desiccation (cells remain viable even after they’ve lost 95% of their water), resurrection plants make ideal models to study the regulation of plant PCD pathways. Previously, we showed that the Australian resurrection plant, Tripogon loliiformis, suppresses plant PCD, via trehalose-mediated activation of autophagy pathways, during drying. In the present study, we created a full-length T. loliiformis cDNA library, performed a large-scale Agrobacterium screen for improved salinity tolerance and identified Stachyose synthase (TlStach) as a potential candidate for improving stress tolerance. Tripogon loliiformis shoots accumulate stachyose synthase transcripts and stachyose during drying. Attempts to generate transgenic plants expressing TlStach failed and were consistent with previous reports in mammals that demonstrated stachyose-mediated induction of apoptosis. Using a combination of transcriptomics, metabolomics and cell death assays (TUNNEL and DNA laddering), we investigated whether stachyose induces apoptotic-like cell death in T. loliiformis. We show that stachyose triggers the formation of the hallmarks of plant apoptotic-like cell death in the desiccation sensitive Nicotiana benthamiana but not the resilient T. loliiformis. These findings suggest that T. loliiformis suppresses stachyose-mediated apoptotic-like cell death and provides insights on the role of sugar metabolism and plant PCD pathways. A better understanding of how resilient plants regulate sugar metabolism and PCD pathways may facilitate future targeting of plant metabolic pathways for increased stress tolerance.
format	article
author	Pauline Okemo Hao Long Yen Cheng Sagadevan Mundree Brett Williams
author_facet	Pauline Okemo Hao Long Yen Cheng Sagadevan Mundree Brett Williams
author_sort	Pauline Okemo
title	Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
title_short	Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
title_full	Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
title_fullStr	Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
title_full_unstemmed	Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
title_sort	stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66b5ca4e4b3a419998348edeb0d28bb4
work_keys_str_mv	AT paulineokemo stachyosetriggersapoptoticlikecelldeathindroughtsensitivebutnotresilientplants AT haolong stachyosetriggersapoptoticlikecelldeathindroughtsensitivebutnotresilientplants AT yencheng stachyosetriggersapoptoticlikecelldeathindroughtsensitivebutnotresilientplants AT sagadevanmundree stachyosetriggersapoptoticlikecelldeathindroughtsensitivebutnotresilientplants AT brettwilliams stachyosetriggersapoptoticlikecelldeathindroughtsensitivebutnotresilientplants
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Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants

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