Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles

Sarah Deville,1,2 Wahyu Wijaya Hadiwikarta,1 Nick Smisdom,1,2 Bart Wathiong,1,3 Marcel Ameloot,2 Inge Nelissen,1 Jef Hooyberghs1,3 1VITO, Flemish Institute for Technological Research, Mol, Belgium; 2Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; 3Theoretical Physics, Hassel...

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Autores principales:	Deville S, Hadiwikarta WW, Smisdom N, Wathiong B, Ameloot M, Nelissen I, Hooyberghs J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanoparticles hematopoietic progenitor cells dendritic cells uptake release Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/66b9f1f378074e778d595b994c4ba5c9
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spelling	oai:doaj.org-article:66b9f1f378074e778d595b994c4ba5c92021-12-02T03:55:35ZTransient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles1178-2013https://doaj.org/article/66b9f1f378074e778d595b994c4ba5c92017-01-01T00:00:00Zhttps://www.dovepress.com/transient-loading-of-cd34-hematopoietic-progenitor-cells-with-polystyr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sarah Deville,1,2 Wahyu Wijaya Hadiwikarta,1 Nick Smisdom,1,2 Bart Wathiong,1,3 Marcel Ameloot,2 Inge Nelissen,1 Jef Hooyberghs1,3 1VITO, Flemish Institute for Technological Research, Mol, Belgium; 2Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; 3Theoretical Physics, Hasselt University, Diepenbeek, Belgium Abstract: CD34+ hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential for a successful application. The present study focusses on the interaction kinetics of 40 nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within 1 hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modeling the kinetics of NP loading and release is presented, contributing to a framework of describing this phenomenon. Keywords: nanoparticles, hematopoietic progenitor cells, dendritic cells, uptake, releaseDeville SHadiwikarta WWSmisdom NWathiong BAmeloot MNelissen IHooyberghs JDove Medical Pressarticlenanoparticleshematopoietic progenitor cellsdendritic cellsuptakereleaseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 459-472 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles hematopoietic progenitor cells dendritic cells uptake release Medicine (General) R5-920
spellingShingle	nanoparticles hematopoietic progenitor cells dendritic cells uptake release Medicine (General) R5-920 Deville S Hadiwikarta WW Smisdom N Wathiong B Ameloot M Nelissen I Hooyberghs J Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
description	Sarah Deville,1,2 Wahyu Wijaya Hadiwikarta,1 Nick Smisdom,1,2 Bart Wathiong,1,3 Marcel Ameloot,2 Inge Nelissen,1 Jef Hooyberghs1,3 1VITO, Flemish Institute for Technological Research, Mol, Belgium; 2Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; 3Theoretical Physics, Hasselt University, Diepenbeek, Belgium Abstract: CD34+ hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential for a successful application. The present study focusses on the interaction kinetics of 40 nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within 1 hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modeling the kinetics of NP loading and release is presented, contributing to a framework of describing this phenomenon. Keywords: nanoparticles, hematopoietic progenitor cells, dendritic cells, uptake, release
format	article
author	Deville S Hadiwikarta WW Smisdom N Wathiong B Ameloot M Nelissen I Hooyberghs J
author_facet	Deville S Hadiwikarta WW Smisdom N Wathiong B Ameloot M Nelissen I Hooyberghs J
author_sort	Deville S
title	Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
title_short	Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
title_full	Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
title_fullStr	Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
title_full_unstemmed	Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
title_sort	transient loading of cd34+ hematopoietic progenitor cells with polystyrene nanoparticles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/66b9f1f378074e778d595b994c4ba5c9
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_version_	1718401553140809728

Transient loading of CD34+&nbsp;hematopoietic progenitor cells with polystyrene nanoparticles

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Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles