Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.

Mitochondrial Ca(2+) uptake, central to neural metabolism and function, is diminished in aging whereas enhanced after acute/sub-acute traumatic brain injury. To develop relevant translational models for these neuropathologies, we determined the impact of perturbed mitochondrial Ca(2+) uptake capacit...

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Autores principales:	Basavaraju G Sanganahalli, Peter Herman, Fahmeed Hyder, Sridhar S Kannurpatti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66bdd89cf2934528a709af5462926dc7
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spelling	oai:doaj.org-article:66bdd89cf2934528a709af5462926dc72021-11-18T07:47:10ZMitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.1932-620310.1371/journal.pone.0063317https://doaj.org/article/66bdd89cf2934528a709af5462926dc72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23650561/?tool=EBIhttps://doaj.org/toc/1932-6203Mitochondrial Ca(2+) uptake, central to neural metabolism and function, is diminished in aging whereas enhanced after acute/sub-acute traumatic brain injury. To develop relevant translational models for these neuropathologies, we determined the impact of perturbed mitochondrial Ca(2+) uptake capacities on intrinsic brain activity using clinically relevant markers. From a multi-compartment estimate of probable baseline Ca(2+) ranges in the brain, we hypothesized that reduced or enhanced mitochondrial Ca(2+) uptake capacity would decrease or increase spontaneous neuronal activity respectively. As resting state fMRI-BOLD fluctuations and stimulus-evoked BOLD responses have similar physiological origins [1] and stimulus-evoked neuronal and hemodynamic responses are modulated by mitochondrial Ca(2+) uptake capacity [2], [3] respectively, we tested our hypothesis by measuring hemodynamic fluctuations and spontaneous neuronal activities during normal and altered mitochondrial functional states. Mitochondrial Ca(2+) uptake capacity was perturbed by pharmacologically inhibiting or enhancing the mitochondrial Ca(2+) uniporter (mCU) activity. Neuronal electrical activity and cerebral blood flow (CBF) fluctuations were measured simultaneously and integrated with fMRI-BOLD fluctuations at 11.7T. mCU inhibition reduced spontaneous neuronal activity and the resting state functional connectivity (RSFC), whereas mCU enhancement increased spontaneous neuronal activity but reduced RSFC. We conclude that increased or decreased mitochondrial Ca(2+) uptake capacities lead to diminished resting state modes of brain functional connectivity.Basavaraju G SanganahalliPeter HermanFahmeed HyderSridhar S KannurpattiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63317 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Basavaraju G Sanganahalli Peter Herman Fahmeed Hyder Sridhar S Kannurpatti Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
description	Mitochondrial Ca(2+) uptake, central to neural metabolism and function, is diminished in aging whereas enhanced after acute/sub-acute traumatic brain injury. To develop relevant translational models for these neuropathologies, we determined the impact of perturbed mitochondrial Ca(2+) uptake capacities on intrinsic brain activity using clinically relevant markers. From a multi-compartment estimate of probable baseline Ca(2+) ranges in the brain, we hypothesized that reduced or enhanced mitochondrial Ca(2+) uptake capacity would decrease or increase spontaneous neuronal activity respectively. As resting state fMRI-BOLD fluctuations and stimulus-evoked BOLD responses have similar physiological origins [1] and stimulus-evoked neuronal and hemodynamic responses are modulated by mitochondrial Ca(2+) uptake capacity [2], [3] respectively, we tested our hypothesis by measuring hemodynamic fluctuations and spontaneous neuronal activities during normal and altered mitochondrial functional states. Mitochondrial Ca(2+) uptake capacity was perturbed by pharmacologically inhibiting or enhancing the mitochondrial Ca(2+) uniporter (mCU) activity. Neuronal electrical activity and cerebral blood flow (CBF) fluctuations were measured simultaneously and integrated with fMRI-BOLD fluctuations at 11.7T. mCU inhibition reduced spontaneous neuronal activity and the resting state functional connectivity (RSFC), whereas mCU enhancement increased spontaneous neuronal activity but reduced RSFC. We conclude that increased or decreased mitochondrial Ca(2+) uptake capacities lead to diminished resting state modes of brain functional connectivity.
format	article
author	Basavaraju G Sanganahalli Peter Herman Fahmeed Hyder Sridhar S Kannurpatti
author_facet	Basavaraju G Sanganahalli Peter Herman Fahmeed Hyder Sridhar S Kannurpatti
author_sort	Basavaraju G Sanganahalli
title	Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
title_short	Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
title_full	Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
title_fullStr	Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
title_full_unstemmed	Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.
title_sort	mitochondrial functional state impacts spontaneous neocortical activity and resting state fmri.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/66bdd89cf2934528a709af5462926dc7
work_keys_str_mv	AT basavarajugsanganahalli mitochondrialfunctionalstateimpactsspontaneousneocorticalactivityandrestingstatefmri AT peterherman mitochondrialfunctionalstateimpactsspontaneousneocorticalactivityandrestingstatefmri AT fahmeedhyder mitochondrialfunctionalstateimpactsspontaneousneocorticalactivityandrestingstatefmri AT sridharskannurpatti mitochondrialfunctionalstateimpactsspontaneousneocorticalactivityandrestingstatefmri
_version_	1718422976050757632

Mitochondrial functional state impacts spontaneous neocortical activity and resting state FMRI.

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