Nano-graphene oxide composite for in vivo imaging

Sung-Chan Jang,1,2,* Sung-Min Kang,1,* Jun Young Lee,3,* Seo Yeong Oh,1 AT Ezhil Vilian,4 Ilsong Lee,1,2 Young-Kyu Han,4 Jeong Hoon Park,3 Wan-Seob Cho,5,* Changhyun Roh,2,6 Yun Suk Huh1 1Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Inche...

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Autores principales:	Jang SC, Kang S, Lee JY, Oh SY, Vilian ATE, Lee I, Han Y, Park JH, Cho WS, Roh C, Huh YS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Graphene oxide nanocomposite Imaging Radiotracer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/66c4b85f65d741789964839d789e5885
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spelling	oai:doaj.org-article:66c4b85f65d741789964839d789e58852021-12-02T02:10:29ZNano-graphene oxide composite for in vivo imaging1178-2013https://doaj.org/article/66c4b85f65d741789964839d789e58852018-01-01T00:00:00Zhttps://www.dovepress.com/nano-graphene-oxide-composite-for-in-vivo-imaging-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sung-Chan Jang,1,2,* Sung-Min Kang,1,* Jun Young Lee,3,* Seo Yeong Oh,1 AT Ezhil Vilian,4 Ilsong Lee,1,2 Young-Kyu Han,4 Jeong Hoon Park,3 Wan-Seob Cho,5,* Changhyun Roh,2,6 Yun Suk Huh1 1Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon, 2Biotechnology Research Division, 3Radiation Instrumentation Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, 4Department of Energy and Materials Engineering, Dongguk University, Seoul, 5Laboratory of Toxicology, Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 6Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST), Daejeon, Republic of Korea *These authors contributed equally to this work Introduction: Positron emission tomography (PET) tracers has the potential to revolutionize cancer imaging and diagnosis. PET tracers offer non-invasive quantitative imaging in biotechnology and biomedical applications, but it requires radioisotopes as radioactive imaging tracers or radiopharmaceuticals. Method: This paper reports the synthesis of 18F-nGO-PEG by covalently functionalizing PEG with nano-graphene oxide, and its excellent stability in physiological solutions. Using a green synthesis route, nGO is then functionalized with a biocompatible PEG polymer to acquire high stability in PBS and DMEM. Results and discussion: The radiochemical safety of 18F-nGO-PEG was measured by a reactive oxygen species and cell viability test. The biodistribution of 18F-nGO-PEG could be observed easily by PET, which suggested the significantly high sensitivity tumor uptake of 18F-nGO-PEG and in a tumor bearing CT-26 mouse compared to the control. 18F-nGO-PEG was applied successfully as an efficient radiotracer or drug agent in vivo using PET imaging. This article is expected to assist many researchers in the fabrication of 18F-labeled graphene-based bio-conjugates with high reproducibility for applications in the biomedicine field. Keywords: graphene oxide, nanocomposite, imaging, radiotracerJang SCKang SLee JYOh SYVilian ATELee IHan YPark JHCho WSRoh CHuh YSDove Medical PressarticleGraphene oxidenanocompositeImagingRadiotracerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 221-234 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene oxide nanocomposite Imaging Radiotracer Medicine (General) R5-920
spellingShingle	Graphene oxide nanocomposite Imaging Radiotracer Medicine (General) R5-920 Jang SC Kang S Lee JY Oh SY Vilian ATE Lee I Han Y Park JH Cho WS Roh C Huh YS Nano-graphene oxide composite for in vivo imaging
description	Sung-Chan Jang,1,2,* Sung-Min Kang,1,* Jun Young Lee,3,* Seo Yeong Oh,1 AT Ezhil Vilian,4 Ilsong Lee,1,2 Young-Kyu Han,4 Jeong Hoon Park,3 Wan-Seob Cho,5,* Changhyun Roh,2,6 Yun Suk Huh1 1Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon, 2Biotechnology Research Division, 3Radiation Instrumentation Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, 4Department of Energy and Materials Engineering, Dongguk University, Seoul, 5Laboratory of Toxicology, Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 6Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST), Daejeon, Republic of Korea *These authors contributed equally to this work Introduction: Positron emission tomography (PET) tracers has the potential to revolutionize cancer imaging and diagnosis. PET tracers offer non-invasive quantitative imaging in biotechnology and biomedical applications, but it requires radioisotopes as radioactive imaging tracers or radiopharmaceuticals. Method: This paper reports the synthesis of 18F-nGO-PEG by covalently functionalizing PEG with nano-graphene oxide, and its excellent stability in physiological solutions. Using a green synthesis route, nGO is then functionalized with a biocompatible PEG polymer to acquire high stability in PBS and DMEM. Results and discussion: The radiochemical safety of 18F-nGO-PEG was measured by a reactive oxygen species and cell viability test. The biodistribution of 18F-nGO-PEG could be observed easily by PET, which suggested the significantly high sensitivity tumor uptake of 18F-nGO-PEG and in a tumor bearing CT-26 mouse compared to the control. 18F-nGO-PEG was applied successfully as an efficient radiotracer or drug agent in vivo using PET imaging. This article is expected to assist many researchers in the fabrication of 18F-labeled graphene-based bio-conjugates with high reproducibility for applications in the biomedicine field. Keywords: graphene oxide, nanocomposite, imaging, radiotracer
format	article
author	Jang SC Kang S Lee JY Oh SY Vilian ATE Lee I Han Y Park JH Cho WS Roh C Huh YS
author_facet	Jang SC Kang S Lee JY Oh SY Vilian ATE Lee I Han Y Park JH Cho WS Roh C Huh YS
author_sort	Jang SC
title	Nano-graphene oxide composite for in vivo imaging
title_short	Nano-graphene oxide composite for in vivo imaging
title_full	Nano-graphene oxide composite for in vivo imaging
title_fullStr	Nano-graphene oxide composite for in vivo imaging
title_full_unstemmed	Nano-graphene oxide composite for in vivo imaging
title_sort	nano-graphene oxide composite for in vivo imaging
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/66c4b85f65d741789964839d789e5885
work_keys_str_mv	AT jangsc nanographeneoxidecompositeforinvivoimaging AT kangs nanographeneoxidecompositeforinvivoimaging AT leejy nanographeneoxidecompositeforinvivoimaging AT ohsy nanographeneoxidecompositeforinvivoimaging AT vilianate nanographeneoxidecompositeforinvivoimaging AT leei nanographeneoxidecompositeforinvivoimaging AT hany nanographeneoxidecompositeforinvivoimaging AT parkjh nanographeneoxidecompositeforinvivoimaging AT chows nanographeneoxidecompositeforinvivoimaging AT rohc nanographeneoxidecompositeforinvivoimaging AT huhys nanographeneoxidecompositeforinvivoimaging
_version_	1718402652634611712

Nano-graphene oxide composite for in vivo imaging

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