Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco

Abstract Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced. Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulat...

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Autores principales:	Yingying Liu, Minjing Wei, Cong Hou, Tingting Lu, Lulu Liu, Hairong Wei, Yuxiang Cheng, Zhigang Wei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66c95c693d5d4fb7b50777baa25d23f5
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spelling	oai:doaj.org-article:66c95c693d5d4fb7b50777baa25d23f52021-12-02T11:40:43ZFunctional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco10.1038/s41598-017-00093-z2045-2322https://doaj.org/article/66c95c693d5d4fb7b50777baa25d23f52017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00093-zhttps://doaj.org/toc/2045-2322Abstract Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced. Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes. Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2. PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator. Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition. The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%. Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors. Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.Yingying LiuMinjing WeiCong HouTingting LuLulu LiuHairong WeiYuxiang ChengZhigang WeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yingying Liu Minjing Wei Cong Hou Tingting Lu Lulu Liu Hairong Wei Yuxiang Cheng Zhigang Wei Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
description	Abstract Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced. Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes. Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2. PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator. Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition. The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%. Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors. Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
format	article
author	Yingying Liu Minjing Wei Cong Hou Tingting Lu Lulu Liu Hairong Wei Yuxiang Cheng Zhigang Wei
author_facet	Yingying Liu Minjing Wei Cong Hou Tingting Lu Lulu Liu Hairong Wei Yuxiang Cheng Zhigang Wei
author_sort	Yingying Liu
title	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
title_short	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
title_full	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
title_fullStr	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
title_full_unstemmed	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco
title_sort	functional characterization of populus psnshn2 in coordinated regulation of secondary wall components in tobacco
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/66c95c693d5d4fb7b50777baa25d23f5
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Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco

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