Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis

Abstract Butyrate is the primary energy source for colonocytes and is essential for mucosal integrity and repair. Butyrate deficiency as a result of colonic dysbiosis is a putative factor in ulcerative colitis (UC). Commensal microbes are butyrogenic, while others may inhibit butyrate, through hydro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Helen Earley, Grainne Lennon, J. Calvin Coffey, Desmond C. Winter, P. Ronan O’Connell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66d0c141823f4f88bf3714160e19779c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:66d0c141823f4f88bf3714160e19779c
record_format	dspace
spelling	oai:doaj.org-article:66d0c141823f4f88bf3714160e19779c2021-12-02T14:23:18ZColonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis10.1038/s41598-021-86166-62045-2322https://doaj.org/article/66d0c141823f4f88bf3714160e19779c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86166-6https://doaj.org/toc/2045-2322Abstract Butyrate is the primary energy source for colonocytes and is essential for mucosal integrity and repair. Butyrate deficiency as a result of colonic dysbiosis is a putative factor in ulcerative colitis (UC). Commensal microbes are butyrogenic, while others may inhibit butyrate, through hydrogenotropic activity. The aim of this study was to quantify butyrogenic and hydrogenotropic species and determine their relationship with inflammation within the colonic mucus gel layer (MGL). Mucosal brushings were obtained from 20 healthy controls (HC), 20 patients with active colitis (AC) and 14 with quiescent colitis (QUC). Abundance of each species was determined by RT-PCR. Inflammatory scores were available for each patient. Statistical analyses were performed using Mann–Whitney-U and Kruskall-Wallis tests. Butyrogenic R. hominis was more abundant in health than UC (p < 0.005), prior to normalisation against total bacteria. Hydrogenotropic B. wadsworthia was reduced in AC compared to HC and QUC (p < 0.005). An inverse correlation existed between inflammation and R. hominis (ρ − 0.460, p < 0.005) and B. wadsworthia (ρ − 0.646, p < 0.005). Other hydrogenotropic species did not widely colonise the MGL. These data support a role for butyrogenic bacteria in UC. Butyrate deficiency in UC may be related to reduced microbial production, rather than inhibition by microbial by-products.Helen EarleyGrainne LennonJ. Calvin CoffeyDesmond C. WinterP. Ronan O’ConnellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Helen Earley Grainne Lennon J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
description	Abstract Butyrate is the primary energy source for colonocytes and is essential for mucosal integrity and repair. Butyrate deficiency as a result of colonic dysbiosis is a putative factor in ulcerative colitis (UC). Commensal microbes are butyrogenic, while others may inhibit butyrate, through hydrogenotropic activity. The aim of this study was to quantify butyrogenic and hydrogenotropic species and determine their relationship with inflammation within the colonic mucus gel layer (MGL). Mucosal brushings were obtained from 20 healthy controls (HC), 20 patients with active colitis (AC) and 14 with quiescent colitis (QUC). Abundance of each species was determined by RT-PCR. Inflammatory scores were available for each patient. Statistical analyses were performed using Mann–Whitney-U and Kruskall-Wallis tests. Butyrogenic R. hominis was more abundant in health than UC (p < 0.005), prior to normalisation against total bacteria. Hydrogenotropic B. wadsworthia was reduced in AC compared to HC and QUC (p < 0.005). An inverse correlation existed between inflammation and R. hominis (ρ − 0.460, p < 0.005) and B. wadsworthia (ρ − 0.646, p < 0.005). Other hydrogenotropic species did not widely colonise the MGL. These data support a role for butyrogenic bacteria in UC. Butyrate deficiency in UC may be related to reduced microbial production, rather than inhibition by microbial by-products.
format	article
author	Helen Earley Grainne Lennon J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell
author_facet	Helen Earley Grainne Lennon J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell
author_sort	Helen Earley
title	Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
title_short	Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
title_full	Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
title_fullStr	Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
title_full_unstemmed	Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
title_sort	colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66d0c141823f4f88bf3714160e19779c
work_keys_str_mv	AT helenearley colonisationofthecolonicmucusgellayerwithbutyrogenicandhydrogenotropicbacteriainhealthandulcerativecolitis AT grainnelennon colonisationofthecolonicmucusgellayerwithbutyrogenicandhydrogenotropicbacteriainhealthandulcerativecolitis AT jcalvincoffey colonisationofthecolonicmucusgellayerwithbutyrogenicandhydrogenotropicbacteriainhealthandulcerativecolitis AT desmondcwinter colonisationofthecolonicmucusgellayerwithbutyrogenicandhydrogenotropicbacteriainhealthandulcerativecolitis AT pronanoconnell colonisationofthecolonicmucusgellayerwithbutyrogenicandhydrogenotropicbacteriainhealthandulcerativecolitis
_version_	1718391430611730432

Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis

Ejemplares similares