Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts

Abstract Ocimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts...

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Autores principales:	Confidence Onyebuchi, Doğa Kavaz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66d4ff02cd2c481f900092600393101e
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spelling	oai:doaj.org-article:66d4ff02cd2c481f900092600393101e2021-12-02T11:43:58ZEffect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts10.1038/s41598-020-78847-52045-2322https://doaj.org/article/66d4ff02cd2c481f900092600393101e2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78847-5https://doaj.org/toc/2045-2322Abstract Ocimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts has not yet been performed. This study adopted different analytical techniques to determine the effect of extraction temperature and solvent type on the phytochemical and bioactive properties of O. gratissimum extracts. Chemical profiling showed increased concentrations of compounds for both the ethanolic and methanolic extracts compared to the water extracts. The results also revealed that the extraction temperature had an effect on the total phenolic content and radical-scavenging properties of the different extracts. The antioxidant kinetic modeling achieved the best fit when using the second-order kinetic model. Methanolic extracts had the highest levels of antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella typhimurium. At high concentrations, all extracts lowered the viability of the breast cancer cell line MDA-MB-231. In conclusion, the chemical and bioactive properties of all extracts showed significant dependence on the extraction temperature and solvent type. With proper extraction methods, they boast a wide range of promising applications in the medical, pharmaceutical, and food industries.Confidence OnyebuchiDoğa KavazNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Confidence Onyebuchi Doğa Kavaz Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
description	Abstract Ocimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts has not yet been performed. This study adopted different analytical techniques to determine the effect of extraction temperature and solvent type on the phytochemical and bioactive properties of O. gratissimum extracts. Chemical profiling showed increased concentrations of compounds for both the ethanolic and methanolic extracts compared to the water extracts. The results also revealed that the extraction temperature had an effect on the total phenolic content and radical-scavenging properties of the different extracts. The antioxidant kinetic modeling achieved the best fit when using the second-order kinetic model. Methanolic extracts had the highest levels of antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella typhimurium. At high concentrations, all extracts lowered the viability of the breast cancer cell line MDA-MB-231. In conclusion, the chemical and bioactive properties of all extracts showed significant dependence on the extraction temperature and solvent type. With proper extraction methods, they boast a wide range of promising applications in the medical, pharmaceutical, and food industries.
format	article
author	Confidence Onyebuchi Doğa Kavaz
author_facet	Confidence Onyebuchi Doğa Kavaz
author_sort	Confidence Onyebuchi
title	Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
title_short	Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
title_full	Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
title_fullStr	Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
title_full_unstemmed	Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts
title_sort	effect of extraction temperature and solvent type on the bioactive potential of ocimum gratissimum l. extracts
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/66d4ff02cd2c481f900092600393101e
work_keys_str_mv	AT confidenceonyebuchi effectofextractiontemperatureandsolventtypeonthebioactivepotentialofocimumgratissimumlextracts AT dogakavaz effectofextractiontemperatureandsolventtypeonthebioactivepotentialofocimumgratissimumlextracts
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Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts

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