Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.

5-methylcytosine (5-mC) can be sequentially oxidized to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-foC), and finally to 5-carboxylcytosine (5-caC), which is thought to function in active DNA cytosine demethylation in mammals. Although the roles of 5-mC in epigenetic regulation of gene expr...

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Autores principales:	Xi-Wen Xing, Yu-Li Liu, Mario Vargas, Yinsheng Wang, Yu-Qi Feng, Xiang Zhou, Bi-Feng Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66d7240d5cd04d0fbd511d3388ea3b33
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spelling	oai:doaj.org-article:66d7240d5cd04d0fbd511d3388ea3b332021-11-18T08:55:15ZMutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.1932-620310.1371/journal.pone.0072993https://doaj.org/article/66d7240d5cd04d0fbd511d3388ea3b332013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24066027/?tool=EBIhttps://doaj.org/toc/1932-62035-methylcytosine (5-mC) can be sequentially oxidized to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-foC), and finally to 5-carboxylcytosine (5-caC), which is thought to function in active DNA cytosine demethylation in mammals. Although the roles of 5-mC in epigenetic regulation of gene expression are well established, the effects of 5-hmC, 5-foC and 5-caC on DNA replication remain unclear. Here we report a systematic study on how these cytosine derivatives (5-hmC, 5-foC and 5-caC) perturb the efficiency and accuracy of DNA replication using shuttle vector technology in conjugation with next-g sequencing. Our results demonstrated that, in Escherichia coli cells, all the cytosine derivatives could induce CT transition mutation at frequencies of 0.17%-1.12%, though no effect on replication efficiency was observed. These findings provide an important new insight on the potential mutagenic properties of cytosine derivatives occurring as the intermediates of DNA demethylation.Xi-Wen XingYu-Li LiuMario VargasYinsheng WangYu-Qi FengXiang ZhouBi-Feng YuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72993 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xi-Wen Xing Yu-Li Liu Mario Vargas Yinsheng Wang Yu-Qi Feng Xiang Zhou Bi-Feng Yuan Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
description	5-methylcytosine (5-mC) can be sequentially oxidized to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-foC), and finally to 5-carboxylcytosine (5-caC), which is thought to function in active DNA cytosine demethylation in mammals. Although the roles of 5-mC in epigenetic regulation of gene expression are well established, the effects of 5-hmC, 5-foC and 5-caC on DNA replication remain unclear. Here we report a systematic study on how these cytosine derivatives (5-hmC, 5-foC and 5-caC) perturb the efficiency and accuracy of DNA replication using shuttle vector technology in conjugation with next-g sequencing. Our results demonstrated that, in Escherichia coli cells, all the cytosine derivatives could induce CT transition mutation at frequencies of 0.17%-1.12%, though no effect on replication efficiency was observed. These findings provide an important new insight on the potential mutagenic properties of cytosine derivatives occurring as the intermediates of DNA demethylation.
format	article
author	Xi-Wen Xing Yu-Li Liu Mario Vargas Yinsheng Wang Yu-Qi Feng Xiang Zhou Bi-Feng Yuan
author_facet	Xi-Wen Xing Yu-Li Liu Mario Vargas Yinsheng Wang Yu-Qi Feng Xiang Zhou Bi-Feng Yuan
author_sort	Xi-Wen Xing
title	Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
title_short	Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
title_full	Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
title_fullStr	Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
title_full_unstemmed	Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
title_sort	mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/66d7240d5cd04d0fbd511d3388ea3b33
work_keys_str_mv	AT xiwenxing mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT yuliliu mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT mariovargas mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT yinshengwang mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT yuqifeng mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT xiangzhou mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing AT bifengyuan mutagenicandcytotoxicpropertiesofoxidationproductsof5methylcytosinerevealedbynextgenerationsequencing
_version_	1718421144185339904

Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing.

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