Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models

The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, animal models contribute a lot to the studies of the mechanisms underlying its pathogenesis. Dietary induction using is a well-accepted protocol in generating insulin resistance an...

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Autores principales:	Zhu Yang, Dianqiang Yang, Fancheng Tan, Chi Wai Wong, James Y. Yang, Da Zhou, Zongwei Cai, Shu-Hai Lin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	spontaneous diabetes mellitus non-human primates cynomolgus monkey (Macaca fascicularis) metabolomics transcriptomics proteomics Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/66d83047092f4d968fbe4929d117f585
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spelling	oai:doaj.org-article:66d83047092f4d968fbe4929d117f5852021-11-22T06:17:44ZMulti-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models1663-981210.3389/fphar.2021.784231https://doaj.org/article/66d83047092f4d968fbe4929d117f5852021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.784231/fullhttps://doaj.org/toc/1663-9812The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, animal models contribute a lot to the studies of the mechanisms underlying its pathogenesis. Dietary induction using is a well-accepted protocol in generating insulin resistance and diabetes models. In the present study, we reported the multi-omics profiling of the liver and sera from both peripheral blood and hepatic portal vein blood from Macaca fascicularis that spontaneously developed Type-2 diabetes mellitus with a chow diet (sDM). The other two groups of the monkeys fed with chow diet and high-fat high-sugar (HFHS) diet, respectively, were included for comparison. Analyses of various omics datasets revealed the alterations of high consistency. Between the sDM and HFHS monkeys, both the similar and unique alterations in the lipid metabolism have been demonstrated from metabolomic, transcriptomic, and proteomic data repeatedly. The comparison of the proteome and transcriptome confirmed the involvement of fatty acid binding protein 4 (FABP4) in the diet-induced pathogenesis of diabetes in macaques. Furthermore, the commonly changed genes between spontaneous diabetes and HFHS diet-induced prediabetes suggested that the alterations in the intra- and extracellular structural proteins and cell migration in the liver might mediate the HFHS diet induction of diabetes mellitus.Zhu YangZhu YangDianqiang YangFancheng TanChi Wai WongJames Y. YangDa ZhouZongwei CaiShu-Hai LinFrontiers Media S.A.articlespontaneous diabetes mellitusnon-human primatescynomolgus monkey (Macaca fascicularis)metabolomicstranscriptomicsproteomicsTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spontaneous diabetes mellitus non-human primates cynomolgus monkey (Macaca fascicularis) metabolomics transcriptomics proteomics Therapeutics. Pharmacology RM1-950
spellingShingle	spontaneous diabetes mellitus non-human primates cynomolgus monkey (Macaca fascicularis) metabolomics transcriptomics proteomics Therapeutics. Pharmacology RM1-950 Zhu Yang Zhu Yang Dianqiang Yang Fancheng Tan Chi Wai Wong James Y. Yang Da Zhou Zongwei Cai Shu-Hai Lin Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
description	The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, animal models contribute a lot to the studies of the mechanisms underlying its pathogenesis. Dietary induction using is a well-accepted protocol in generating insulin resistance and diabetes models. In the present study, we reported the multi-omics profiling of the liver and sera from both peripheral blood and hepatic portal vein blood from Macaca fascicularis that spontaneously developed Type-2 diabetes mellitus with a chow diet (sDM). The other two groups of the monkeys fed with chow diet and high-fat high-sugar (HFHS) diet, respectively, were included for comparison. Analyses of various omics datasets revealed the alterations of high consistency. Between the sDM and HFHS monkeys, both the similar and unique alterations in the lipid metabolism have been demonstrated from metabolomic, transcriptomic, and proteomic data repeatedly. The comparison of the proteome and transcriptome confirmed the involvement of fatty acid binding protein 4 (FABP4) in the diet-induced pathogenesis of diabetes in macaques. Furthermore, the commonly changed genes between spontaneous diabetes and HFHS diet-induced prediabetes suggested that the alterations in the intra- and extracellular structural proteins and cell migration in the liver might mediate the HFHS diet induction of diabetes mellitus.
format	article
author	Zhu Yang Zhu Yang Dianqiang Yang Fancheng Tan Chi Wai Wong James Y. Yang Da Zhou Zongwei Cai Shu-Hai Lin
author_facet	Zhu Yang Zhu Yang Dianqiang Yang Fancheng Tan Chi Wai Wong James Y. Yang Da Zhou Zongwei Cai Shu-Hai Lin
author_sort	Zhu Yang
title	Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
title_short	Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
title_full	Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
title_fullStr	Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
title_full_unstemmed	Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models
title_sort	multi-omics comparison of the spontaneous diabetes mellitus and diet-induced prediabetic macaque models
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/66d83047092f4d968fbe4929d117f585
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Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models

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