Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples

Abstract Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains...

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Autores principales:	Nicole Tueni, Jérémy Vizet, Martin Genet, Angelo Pierangelo, Jean-Marc Allain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66da1ab0cfd847a388cdc14c0a289121
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spelling	oai:doaj.org-article:66da1ab0cfd847a388cdc14c0a2891212021-12-02T15:09:48ZMicrostructural deformation observed by Mueller polarimetry during traction assay on myocardium samples10.1038/s41598-020-76820-w2045-2322https://doaj.org/article/66da1ab0cfd847a388cdc14c0a2891212020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76820-whttps://doaj.org/toc/2045-2322Abstract Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains to perform multiscale observations of the mechanical response of the heart wall. For this purpose, in this study, a full-field Mueller polarimetric imager (MPI) was combined, for the first time, with an in-situ traction device. The full-field MPI enables to obtain a macroscopic image of the explored tissue, while providing detailed information about its structure on a microscopic scale. Specifically it exploits the polarization of the light to determine various biophysical quantities related to the tissue scattering or anisotropy properties. Combined with a mechanical traction device, the full-field MPI allows to measure the evolution of such biophysical quantities during tissue stretch. We observe separation lines on the tissue, which are associated with a fast variation of the fiber orientation, and have the size of cardiomyocyte bundles. Thus, we hypothesize that these lines are the perimysium, the collagen layer surrounding these bundles. During the mechanical traction, we observe two mechanisms simultaneously. On one hand, the azimuth shows an affine behavior, meaning the orientation changes according to the tissue deformation, and showing coherence in the tissue. On the other hand, the separation lines appear to be resistant in shear and compression but weak against traction, with a forming of gaps in the tissue.Nicole TueniJérémy VizetMartin GenetAngelo PierangeloJean-Marc AllainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicole Tueni Jérémy Vizet Martin Genet Angelo Pierangelo Jean-Marc Allain Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
description	Abstract Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains to perform multiscale observations of the mechanical response of the heart wall. For this purpose, in this study, a full-field Mueller polarimetric imager (MPI) was combined, for the first time, with an in-situ traction device. The full-field MPI enables to obtain a macroscopic image of the explored tissue, while providing detailed information about its structure on a microscopic scale. Specifically it exploits the polarization of the light to determine various biophysical quantities related to the tissue scattering or anisotropy properties. Combined with a mechanical traction device, the full-field MPI allows to measure the evolution of such biophysical quantities during tissue stretch. We observe separation lines on the tissue, which are associated with a fast variation of the fiber orientation, and have the size of cardiomyocyte bundles. Thus, we hypothesize that these lines are the perimysium, the collagen layer surrounding these bundles. During the mechanical traction, we observe two mechanisms simultaneously. On one hand, the azimuth shows an affine behavior, meaning the orientation changes according to the tissue deformation, and showing coherence in the tissue. On the other hand, the separation lines appear to be resistant in shear and compression but weak against traction, with a forming of gaps in the tissue.
format	article
author	Nicole Tueni Jérémy Vizet Martin Genet Angelo Pierangelo Jean-Marc Allain
author_facet	Nicole Tueni Jérémy Vizet Martin Genet Angelo Pierangelo Jean-Marc Allain
author_sort	Nicole Tueni
title	Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
title_short	Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
title_full	Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
title_fullStr	Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
title_full_unstemmed	Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples
title_sort	microstructural deformation observed by mueller polarimetry during traction assay on myocardium samples
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/66da1ab0cfd847a388cdc14c0a289121
work_keys_str_mv	AT nicoletueni microstructuraldeformationobservedbymuellerpolarimetryduringtractionassayonmyocardiumsamples AT jeremyvizet microstructuraldeformationobservedbymuellerpolarimetryduringtractionassayonmyocardiumsamples AT martingenet microstructuraldeformationobservedbymuellerpolarimetryduringtractionassayonmyocardiumsamples AT angelopierangelo microstructuraldeformationobservedbymuellerpolarimetryduringtractionassayonmyocardiumsamples AT jeanmarcallain microstructuraldeformationobservedbymuellerpolarimetryduringtractionassayonmyocardiumsamples
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Microstructural deformation observed by Mueller polarimetry during traction assay on myocardium samples

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