A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide

Changhyun RohDivision of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, Republic of KoreaAbstract: Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global d...

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Autor principal:	Roh C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/66dbf496b10a41c5bd8620d5cba153ad
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spelling	oai:doaj.org-article:66dbf496b10a41c5bd8620d5cba153ad2021-12-02T00:48:22ZA facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide1176-91141178-2013https://doaj.org/article/66dbf496b10a41c5bd8620d5cba153ad2012-05-01T00:00:00Zhttp://www.dovepress.com/a-facile-inhibitor-screening-of-sars-coronavirus-n-protein-using-nanop-a9810https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Changhyun RohDivision of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, Republic of KoreaAbstract: Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of the SARS-associated coronavirus (SARS-CoV) nucleocapsid (N) protein. In this study, we found that a designed biochip could analyze inhibitors of the SARS-CoV N protein using nanoparticle-based RNA oligonucleotide. Among the polyphenolic compounds examined, (-)-catechin gallate and (-)-gallocatechin gallate demonstrated a remarkable inhibition activity on SARS-CoV N protein. (-)-catechin gallate and (-)-gallocatechin gallate attenuated the binding affinity in a concentrated manner as evidenced by QDs-conjugated RNA oligonucleotide on a designed biochip. At a concentration of 0.05 &micro;g mL&ndash;1, (-)-catechin gallate and (-)-gallocatechin gallate showed more than 40% inhibition activity on a nanoparticle-based RNA oligonucleotide biochip system.Keywords: SARS, RNA oligonucleotide, quantum dots, inhibitor, screeningRoh CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2173-2179 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Roh C A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
description	Changhyun RohDivision of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, Republic of KoreaAbstract: Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of the SARS-associated coronavirus (SARS-CoV) nucleocapsid (N) protein. In this study, we found that a designed biochip could analyze inhibitors of the SARS-CoV N protein using nanoparticle-based RNA oligonucleotide. Among the polyphenolic compounds examined, (-)-catechin gallate and (-)-gallocatechin gallate demonstrated a remarkable inhibition activity on SARS-CoV N protein. (-)-catechin gallate and (-)-gallocatechin gallate attenuated the binding affinity in a concentrated manner as evidenced by QDs-conjugated RNA oligonucleotide on a designed biochip. At a concentration of 0.05 &micro;g mL&ndash;1, (-)-catechin gallate and (-)-gallocatechin gallate showed more than 40% inhibition activity on a nanoparticle-based RNA oligonucleotide biochip system.Keywords: SARS, RNA oligonucleotide, quantum dots, inhibitor, screening
format	article
author	Roh C
author_facet	Roh C
author_sort	Roh C
title	A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
title_short	A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
title_full	A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
title_fullStr	A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
title_full_unstemmed	A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide
title_sort	facile inhibitor screening of sars coronavirus n protein using nanoparticle-based rna oligonucleotide
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/66dbf496b10a41c5bd8620d5cba153ad
work_keys_str_mv	AT rohc afacileinhibitorscreeningofsarscoronavirusnproteinusingnanoparticlebasedrnaoligonucleotide AT rohc facileinhibitorscreeningofsarscoronavirusnproteinusingnanoparticlebasedrnaoligonucleotide
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A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide

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