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Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measu...

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Autores principales: Yeanchon H. Dulanlebit, Nikmans Hattu, Ritti P. Sangadji, Risma Mahulette, Nataniel Kelmanutu, Sandriliati Asari
Formato: article
Lenguaje:EN
Publicado: University of Brawijaya 2020
Materias:
caffeine
hplc
iodometry
robusta sp
spectrophotometry
theobroma cacao l
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/66dd9737b0d3408e883b5b1adc61a839
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spelling oai:doaj.org-article:66dd9737b0d3408e883b5b1adc61a8392021-11-24T10:26:44ZArticle Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography2302-46902541-0733https://doaj.org/article/66dd9737b0d3408e883b5b1adc61a8392020-12-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/535/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2541-0733Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The performance of UV-Vis spectrophotometry gives linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%.Yeanchon H. Dulanlebit Nikmans HattuRitti P. SangadjiRisma Mahulette Nataniel KelmanutuSandriliati AsariUniversity of Brawijayaarticlecaffeinehplciodometryrobusta spspectrophotometrytheobroma cacao lChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 09, Iss 3, Pp 212-221 (2020)
institution DOAJ
collection DOAJ
language EN
topic caffeine
hplc
iodometry
robusta sp
spectrophotometry
theobroma cacao l
Chemistry
QD1-999
spellingShingle caffeine
hplc
iodometry
robusta sp
spectrophotometry
theobroma cacao l
Chemistry
QD1-999
Yeanchon H. Dulanlebit
Nikmans Hattu
Ritti P. Sangadji
Risma Mahulette
Nataniel Kelmanutu
Sandriliati Asari
Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
description Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The performance of UV-Vis spectrophotometry gives linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%.
format article
author Yeanchon H. Dulanlebit
Nikmans Hattu
Ritti P. Sangadji
Risma Mahulette
Nataniel Kelmanutu
Sandriliati Asari
author_facet Yeanchon H. Dulanlebit
Nikmans Hattu
Ritti P. Sangadji
Risma Mahulette
Nataniel Kelmanutu
Sandriliati Asari
author_sort Yeanchon H. Dulanlebit
title Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
title_short Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
title_full Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
title_fullStr Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
title_full_unstemmed Article Tools Print this article Indexing metadata How to cite item Email this article (Login required) Email the author (Login required) About JPACR Aim and Scope Editorial Team Publication Ethics Visitor Statistics Guidelines Author Guidelines Online Submission Template Download Template : Article Template format .doc (Ms. Word 97-2003) Article Template format .docx (Ms. Word 2010) Article Template format .rtf Journal Index User Username fathiarahma Password •••••••••••••• Remember me Notifications View Subscribe Journal Content Search Search Scope All Browse By Issue By Author By Title Information For Readers For Authors For Librarians Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
title_sort article tools print this article indexing metadata how to cite item email this article (login required) email the author (login required) about jpacr aim and scope editorial team publication ethics visitor statistics guidelines author guidelines online submission template download template : article template format .doc (ms. word 97-2003) article template format .docx (ms. word 2010) article template format .rtf journal index user username fathiarahma password •••••••••••••• remember me notifications view subscribe journal content search search scope all browse by issue by author by title information for readers for authors for librarians performance of caffeine content analysis in robusta sp and theobroma cacao l using iodometry, uv-vis spectrophotometry and high performance liquid chromatography
publisher University of Brawijaya
publishDate 2020
url https://doaj.org/article/66dd9737b0d3408e883b5b1adc61a839
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