Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia

Abstract. Kusdianawati, Mustopa AZ, Fatimah, Budiarto BR. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiversitas 21: 3225-3233. LAB from Sumbawa horse milk has good potential antimicrobial and probiotic agents. It is known, the study on LAB diversi...

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Autores principales:	kusdianawati KUSDIANAWATI, Apon Zaenal MUSTOPA, Fatimah FATIMAH, Bugi Ratno Budiarto
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna analysis lab rapd-pcr analysis sumbawa horse milk Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/66e3316f04c944a6aee0c7864a87f538
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spelling	oai:doaj.org-article:66e3316f04c944a6aee0c7864a87f5382021-11-22T00:40:51ZGenetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia1412-033X2085-472210.13057/biodiv/d210744https://doaj.org/article/66e3316f04c944a6aee0c7864a87f5382020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6000https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kusdianawati, Mustopa AZ, Fatimah, Budiarto BR. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiversitas 21: 3225-3233. LAB from Sumbawa horse milk has good potential antimicrobial and probiotic agents. It is known, the study on LAB diversity based on its phenotypic characters is difficult to be distinguished. However, the development of molecular characterization based on the genotypic characteristic could be done for LAB diversity analysis. The aim of this study is to obtain the genetic diversity of LAB from Sumbawa horse milk collected from Penyaring Village and Lennanguar Village, Sumbawa, West Nusa Tenggara Indonesia. LAB strains were identified based on their genotypic characteristics, including their randomly amplified polymorphic DNA (RAPD) primers profiles and 16S ribosomal RNA (rRNA) sequences. The result of RAPD-PCR analysis showed 5 clusters of dendrograms resulted from GTG5 and LB2 primer amplification. Based on 16 rRNA sequences result, the phylogenetic tree was constructed and revealed 7 species of LAB i.e: SK 1.5, SKP K.3, SKP K.5, SKP K.9/SKP K.7/M.SKP K.3, SKL K.4, M.SKL K.1/ M.SKL K.5, and SKP K.4 belonging to the species of Enterococcus faecium, Weissella confusa, Lactococcus garvieae, Enterococcus thailandicus, Lactobacillus fermentum, Enterococcus faecalis, and Lactococcus petauri. In this study, the bacteria from Enterococcus sp., Lactococcus garvieae, and Lactococcus petauri existed as a novel of bacteria which means they have not been isolated and identified in Sumbawa horse milk compared to the previous findings.kusdianawati KUSDIANAWATIApon Zaenal MUSTOPAFatimah FATIMAHBugi Ratno BudiartoMBI & UNS Soloarticle16s rrna analysislabrapd-pcr analysissumbawa horse milkBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna analysis lab rapd-pcr analysis sumbawa horse milk Biology (General) QH301-705.5
spellingShingle	16s rrna analysis lab rapd-pcr analysis sumbawa horse milk Biology (General) QH301-705.5 kusdianawati KUSDIANAWATI Apon Zaenal MUSTOPA Fatimah FATIMAH Bugi Ratno Budiarto Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
description	Abstract. Kusdianawati, Mustopa AZ, Fatimah, Budiarto BR. 2020. Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia. Biodiversitas 21: 3225-3233. LAB from Sumbawa horse milk has good potential antimicrobial and probiotic agents. It is known, the study on LAB diversity based on its phenotypic characters is difficult to be distinguished. However, the development of molecular characterization based on the genotypic characteristic could be done for LAB diversity analysis. The aim of this study is to obtain the genetic diversity of LAB from Sumbawa horse milk collected from Penyaring Village and Lennanguar Village, Sumbawa, West Nusa Tenggara Indonesia. LAB strains were identified based on their genotypic characteristics, including their randomly amplified polymorphic DNA (RAPD) primers profiles and 16S ribosomal RNA (rRNA) sequences. The result of RAPD-PCR analysis showed 5 clusters of dendrograms resulted from GTG5 and LB2 primer amplification. Based on 16 rRNA sequences result, the phylogenetic tree was constructed and revealed 7 species of LAB i.e: SK 1.5, SKP K.3, SKP K.5, SKP K.9/SKP K.7/M.SKP K.3, SKL K.4, M.SKL K.1/ M.SKL K.5, and SKP K.4 belonging to the species of Enterococcus faecium, Weissella confusa, Lactococcus garvieae, Enterococcus thailandicus, Lactobacillus fermentum, Enterococcus faecalis, and Lactococcus petauri. In this study, the bacteria from Enterococcus sp., Lactococcus garvieae, and Lactococcus petauri existed as a novel of bacteria which means they have not been isolated and identified in Sumbawa horse milk compared to the previous findings.
format	article
author	kusdianawati KUSDIANAWATI Apon Zaenal MUSTOPA Fatimah FATIMAH Bugi Ratno Budiarto
author_facet	kusdianawati KUSDIANAWATI Apon Zaenal MUSTOPA Fatimah FATIMAH Bugi Ratno Budiarto
author_sort	kusdianawati KUSDIANAWATI
title	Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
title_short	Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
title_full	Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
title_fullStr	Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
title_full_unstemmed	Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia
title_sort	genetic diversity of lactic acid bacteria isolated from sumbawa horse milk, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/66e3316f04c944a6aee0c7864a87f538
work_keys_str_mv	AT kusdianawatikusdianawati geneticdiversityoflacticacidbacteriaisolatedfromsumbawahorsemilkindonesia AT aponzaenalmustopa geneticdiversityoflacticacidbacteriaisolatedfromsumbawahorsemilkindonesia AT fatimahfatimah geneticdiversityoflacticacidbacteriaisolatedfromsumbawahorsemilkindonesia AT bugiratnobudiarto geneticdiversityoflacticacidbacteriaisolatedfromsumbawahorsemilkindonesia
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Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia

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