Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry

The disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-ci...

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Autores principales:	Dolly L. Crawford, Alexis R. Phillips, Taylor R. Williams
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Electronic cigarette Metabolism Respiratory exchange ratio Indirect calorimetry C57BL/6J mice Gender Physiology QP1-981 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/66f21b6b7fd14c31adb258340c3dcd43
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spelling	oai:doaj.org-article:66f21b6b7fd14c31adb258340c3dcd432021-11-28T04:37:22ZEvaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry2589-936810.1016/j.metop.2021.100150https://doaj.org/article/66f21b6b7fd14c31adb258340c3dcd432021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589936821000748https://doaj.org/toc/2589-9368The disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-cigarette vapor in a 1L chamber to test the hypothesis that lipid metabolism predominates in vaped mice. Metabolism was quantified via RER using a GA-200 gas analyzer (iWorx, Inc) and LabScribe v.4 (iWorx, Inc.) software. Blood glucose levels were assessed from a subset of the population using an Accu-Check glucometer (Roche Diagnostics, Inc.). Statistical analyses were conducted using R v.4.0.3. Median RER for controls was lower in females. Older females showed a reduction in RER when exposure occurred in the afternoon (p < 0.001), and in males when exposure occurred in the morning (p = 0.007). Glucose concentrations (mg/dL) were higher after e-cigarette inhalation compared with controls, but this difference was not significant (p = 0.464). The reduction in the respiratory exchange ratio supports the hypothesis that e-cigarette inhalation promotes lipid metabolism, and the magnitude of the effect is influenced by gender, age and time of day.Dolly L. CrawfordAlexis R. PhillipsTaylor R. WilliamsElsevierarticleElectronic cigaretteMetabolismRespiratory exchange ratioIndirect calorimetryC57BL/6J miceGenderPhysiologyQP1-981BiochemistryQD415-436ENMetabolism Open, Vol 12, Iss , Pp 100150- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronic cigarette Metabolism Respiratory exchange ratio Indirect calorimetry C57BL/6J mice Gender Physiology QP1-981 Biochemistry QD415-436
spellingShingle	Electronic cigarette Metabolism Respiratory exchange ratio Indirect calorimetry C57BL/6J mice Gender Physiology QP1-981 Biochemistry QD415-436 Dolly L. Crawford Alexis R. Phillips Taylor R. Williams Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
description	The disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-cigarette vapor in a 1L chamber to test the hypothesis that lipid metabolism predominates in vaped mice. Metabolism was quantified via RER using a GA-200 gas analyzer (iWorx, Inc) and LabScribe v.4 (iWorx, Inc.) software. Blood glucose levels were assessed from a subset of the population using an Accu-Check glucometer (Roche Diagnostics, Inc.). Statistical analyses were conducted using R v.4.0.3. Median RER for controls was lower in females. Older females showed a reduction in RER when exposure occurred in the afternoon (p < 0.001), and in males when exposure occurred in the morning (p = 0.007). Glucose concentrations (mg/dL) were higher after e-cigarette inhalation compared with controls, but this difference was not significant (p = 0.464). The reduction in the respiratory exchange ratio supports the hypothesis that e-cigarette inhalation promotes lipid metabolism, and the magnitude of the effect is influenced by gender, age and time of day.
format	article
author	Dolly L. Crawford Alexis R. Phillips Taylor R. Williams
author_facet	Dolly L. Crawford Alexis R. Phillips Taylor R. Williams
author_sort	Dolly L. Crawford
title	Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
title_short	Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
title_full	Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
title_fullStr	Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
title_full_unstemmed	Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
title_sort	evaluation of secondary electronic cigarette inhalation on lipid metabolism in c57bl/6j mice using indirect calorimetry
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/66f21b6b7fd14c31adb258340c3dcd43
work_keys_str_mv	AT dollylcrawford evaluationofsecondaryelectroniccigaretteinhalationonlipidmetabolisminc57bl6jmiceusingindirectcalorimetry AT alexisrphillips evaluationofsecondaryelectroniccigaretteinhalationonlipidmetabolisminc57bl6jmiceusingindirectcalorimetry AT taylorrwilliams evaluationofsecondaryelectroniccigaretteinhalationonlipidmetabolisminc57bl6jmiceusingindirectcalorimetry
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Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry

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