Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles

Pierson Rathinaraj,1 Ahmed M Al-Jumaily,1 Do Sung Huh21Institute of Biomedical Technologies, Auckland University of Technology, Auckland, New Zealand; 2Department of Nano science and Engineering, Inje University, Gimhea, South KoreaAbstract: Herceptin, the monoclonal antibody, was successfully immo...

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Autores principales:	Rathinaraj P, Al-Jumaily AM, Huh DS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/66f27d0025b744dc9eaa3a4037fdbb22
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spelling	oai:doaj.org-article:66f27d0025b744dc9eaa3a4037fdbb222021-12-02T02:18:14ZInternalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles1179-1314https://doaj.org/article/66f27d0025b744dc9eaa3a4037fdbb222015-02-01T00:00:00Zhttp://www.dovepress.com/internalization-acute-apoptosis-of-breast-cancer-cells-using-herceptin-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314 Pierson Rathinaraj,1 Ahmed M Al-Jumaily,1 Do Sung Huh21Institute of Biomedical Technologies, Auckland University of Technology, Auckland, New Zealand; 2Department of Nano science and Engineering, Inje University, Gimhea, South KoreaAbstract: Herceptin, the monoclonal antibody, was successfully immobilized on gold nanoparticles (GNPs) to improve their precise interactions with breast cancer cells (SK-BR3). The mean size of the GNPs (29 nm), as determined by dynamic light scattering, enlarged to 82 nm after herceptin immobilization. The in vitro cell culture experiment indicated that human skin cells (FB) proliferated well in the presence of herceptin-conjugated GNP (GNP–Her), while most of the breast cancer cells (SK-BR3) had died. To elucidate the mechanism of cell death, the interaction of breast cancer cells with GNP–Her was tracked by confocal laser scanning microscopy. Consequently, GNP–Her was found to be bound precisely to the membrane of the breast cancer cell, which became almost saturated after 6 hours incubation. This shows that the progression signal of SK-BR3 cells is retarded completely by the precise binding of antibody to the human epidermal growth factor receptor 2 receptor of the breast cancer cell membrane, causing cell death.Keywords: herceptin, gold nanoparticles, SK-BR3 cells, intracellular uptakeRathinaraj PAl-Jumaily AMHuh DSDove Medical PressarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol 2015, Iss default, Pp 51-58 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Rathinaraj P Al-Jumaily AM Huh DS Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
description	Pierson Rathinaraj,1 Ahmed M Al-Jumaily,1 Do Sung Huh21Institute of Biomedical Technologies, Auckland University of Technology, Auckland, New Zealand; 2Department of Nano science and Engineering, Inje University, Gimhea, South KoreaAbstract: Herceptin, the monoclonal antibody, was successfully immobilized on gold nanoparticles (GNPs) to improve their precise interactions with breast cancer cells (SK-BR3). The mean size of the GNPs (29 nm), as determined by dynamic light scattering, enlarged to 82 nm after herceptin immobilization. The in vitro cell culture experiment indicated that human skin cells (FB) proliferated well in the presence of herceptin-conjugated GNP (GNP–Her), while most of the breast cancer cells (SK-BR3) had died. To elucidate the mechanism of cell death, the interaction of breast cancer cells with GNP–Her was tracked by confocal laser scanning microscopy. Consequently, GNP–Her was found to be bound precisely to the membrane of the breast cancer cell, which became almost saturated after 6 hours incubation. This shows that the progression signal of SK-BR3 cells is retarded completely by the precise binding of antibody to the human epidermal growth factor receptor 2 receptor of the breast cancer cell membrane, causing cell death.Keywords: herceptin, gold nanoparticles, SK-BR3 cells, intracellular uptake
format	article
author	Rathinaraj P Al-Jumaily AM Huh DS
author_facet	Rathinaraj P Al-Jumaily AM Huh DS
author_sort	Rathinaraj P
title	Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
title_short	Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
title_full	Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
title_fullStr	Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
title_full_unstemmed	Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
title_sort	internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/66f27d0025b744dc9eaa3a4037fdbb22
work_keys_str_mv	AT rathinarajp internalizationacuteapoptosisofbreastcancercellsusingherceptinimmobilizedgoldnanoparticles AT aljumailyam internalizationacuteapoptosisofbreastcancercellsusingherceptinimmobilizedgoldnanoparticles AT huhds internalizationacuteapoptosisofbreastcancercellsusingherceptinimmobilizedgoldnanoparticles
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Internalization: acute apoptosis of breast cancer cells using herceptin-immobilized gold nanoparticles

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