Collagen and elastic fibres in acute and chronic liver injury

Abstract The histological distinction between acute and chronic liver injury is a challenging aspect of liver histopathology. It is traditionally based on the interpretation of morphological changes to the extracellular matrix (ECM) at sites of hepatocyte loss using histochemical stains. Our aim was...

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Autores principales:	Andrew Hall, Corina Cotoi, Tu Vinh Luong, Jennifer Watkins, Prithi Bhathal, Alberto Quaglia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/66f92431e9684711a334667c8e4a59d0
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spelling	oai:doaj.org-article:66f92431e9684711a334667c8e4a59d02021-12-02T15:33:12ZCollagen and elastic fibres in acute and chronic liver injury10.1038/s41598-021-93566-12045-2322https://doaj.org/article/66f92431e9684711a334667c8e4a59d02021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93566-1https://doaj.org/toc/2045-2322Abstract The histological distinction between acute and chronic liver injury is a challenging aspect of liver histopathology. It is traditionally based on the interpretation of morphological changes to the extracellular matrix (ECM) at sites of hepatocyte loss using histochemical stains. Our aim was to investigate whether immunohistochemistry and multiplexing for collagen type (I & III) and elastic fibres and a modified Victoria blue method could be helpful. We studied 43 livers removed at transplantation for acute liver failure (ALF, 20 cases) or cirrhosis (23) plus 8 normal controls. In ALF the periportal ECM was normal in 2 cases, contained mainly collagen I associated with a ductular reaction in 6 cases, and delicate elastic strands in 11 cases. Periportal deposition of mainly collagen I and mature elastic fibres was observed in cirrhosis. In ALF the perisinusoidal ECM was intact in 4 cases, collapsed or condensed but of normal composition (predominantly collagen III) in 2 cases, and collapsed and condensed containing mostly collagen I in 17 cases (7 including delicate immature elastic strands). In contrast, bridging fibrous septa of cirrhosis contained abundant collagen 1 and bundles of mature elastin. We propose the use of a scale and the use of immunohistochemistry and multiplexing in additional to histochemical stains to characterise the ECM changes in acute and chronic liver injury.Andrew HallCorina CotoiTu Vinh LuongJennifer WatkinsPrithi BhathalAlberto QuagliaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrew Hall Corina Cotoi Tu Vinh Luong Jennifer Watkins Prithi Bhathal Alberto Quaglia Collagen and elastic fibres in acute and chronic liver injury
description	Abstract The histological distinction between acute and chronic liver injury is a challenging aspect of liver histopathology. It is traditionally based on the interpretation of morphological changes to the extracellular matrix (ECM) at sites of hepatocyte loss using histochemical stains. Our aim was to investigate whether immunohistochemistry and multiplexing for collagen type (I & III) and elastic fibres and a modified Victoria blue method could be helpful. We studied 43 livers removed at transplantation for acute liver failure (ALF, 20 cases) or cirrhosis (23) plus 8 normal controls. In ALF the periportal ECM was normal in 2 cases, contained mainly collagen I associated with a ductular reaction in 6 cases, and delicate elastic strands in 11 cases. Periportal deposition of mainly collagen I and mature elastic fibres was observed in cirrhosis. In ALF the perisinusoidal ECM was intact in 4 cases, collapsed or condensed but of normal composition (predominantly collagen III) in 2 cases, and collapsed and condensed containing mostly collagen I in 17 cases (7 including delicate immature elastic strands). In contrast, bridging fibrous septa of cirrhosis contained abundant collagen 1 and bundles of mature elastin. We propose the use of a scale and the use of immunohistochemistry and multiplexing in additional to histochemical stains to characterise the ECM changes in acute and chronic liver injury.
format	article
author	Andrew Hall Corina Cotoi Tu Vinh Luong Jennifer Watkins Prithi Bhathal Alberto Quaglia
author_facet	Andrew Hall Corina Cotoi Tu Vinh Luong Jennifer Watkins Prithi Bhathal Alberto Quaglia
author_sort	Andrew Hall
title	Collagen and elastic fibres in acute and chronic liver injury
title_short	Collagen and elastic fibres in acute and chronic liver injury
title_full	Collagen and elastic fibres in acute and chronic liver injury
title_fullStr	Collagen and elastic fibres in acute and chronic liver injury
title_full_unstemmed	Collagen and elastic fibres in acute and chronic liver injury
title_sort	collagen and elastic fibres in acute and chronic liver injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/66f92431e9684711a334667c8e4a59d0
work_keys_str_mv	AT andrewhall collagenandelasticfibresinacuteandchronicliverinjury AT corinacotoi collagenandelasticfibresinacuteandchronicliverinjury AT tuvinhluong collagenandelasticfibresinacuteandchronicliverinjury AT jenniferwatkins collagenandelasticfibresinacuteandchronicliverinjury AT prithibhathal collagenandelasticfibresinacuteandchronicliverinjury AT albertoquaglia collagenandelasticfibresinacuteandchronicliverinjury
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Collagen and elastic fibres in acute and chronic liver injury

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