Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1

<i>Paenibacilli</i> are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to...

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Autores principales:	Pascal Mülner, Elisa Schwarz, Kristin Dietel, Stefanie Herfort, Jennifer Jähne, Peter Lasch, Tomislav Cernava, Gabriele Berg, Joachim Vater
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>P. polymyxa</i> bioactive peptides volatiles MALDI-TOF mass spectrometry GC–MS Medicine R
Acceso en línea:	https://doaj.org/article/66fc76f8632e45959828089ec4d5d7e0
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id	oai:doaj.org-article:66fc76f8632e45959828089ec4d5d7e0
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spelling	oai:doaj.org-article:66fc76f8632e45959828089ec4d5d7e02021-11-25T18:38:37ZFusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M110.3390/pathogens101114852076-0817https://doaj.org/article/66fc76f8632e45959828089ec4d5d7e02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1485https://doaj.org/toc/2076-0817<i>Paenibacilli</i> are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to inactivate pathogens. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by <i>Paenibacillus polymyxa</i> strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variability. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains, novel but not yet completely characterized polymyxin species were detected, which possibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins have gained increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC–MS approach using different cultivation media. Production of volatile organic compounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an efficient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of <i>Paenibacilli</i> is the formation of volatile pyrazine derivatives.Pascal MülnerElisa SchwarzKristin DietelStefanie HerfortJennifer JähnePeter LaschTomislav CernavaGabriele BergJoachim VaterMDPI AGarticle<i>P. polymyxa</i>bioactive peptidesvolatilesMALDI-TOF mass spectrometryGC–MSMedicineRENPathogens, Vol 10, Iss 1485, p 1485 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>P. polymyxa</i> bioactive peptides volatiles MALDI-TOF mass spectrometry GC–MS Medicine R
spellingShingle	<i>P. polymyxa</i> bioactive peptides volatiles MALDI-TOF mass spectrometry GC–MS Medicine R Pascal Mülner Elisa Schwarz Kristin Dietel Stefanie Herfort Jennifer Jähne Peter Lasch Tomislav Cernava Gabriele Berg Joachim Vater Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
description	<i>Paenibacilli</i> are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to inactivate pathogens. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by <i>Paenibacillus polymyxa</i> strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variability. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains, novel but not yet completely characterized polymyxin species were detected, which possibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins have gained increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC–MS approach using different cultivation media. Production of volatile organic compounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an efficient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of <i>Paenibacilli</i> is the formation of volatile pyrazine derivatives.
format	article
author	Pascal Mülner Elisa Schwarz Kristin Dietel Stefanie Herfort Jennifer Jähne Peter Lasch Tomislav Cernava Gabriele Berg Joachim Vater
author_facet	Pascal Mülner Elisa Schwarz Kristin Dietel Stefanie Herfort Jennifer Jähne Peter Lasch Tomislav Cernava Gabriele Berg Joachim Vater
author_sort	Pascal Mülner
title	Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
title_short	Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
title_full	Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
title_fullStr	Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
title_full_unstemmed	Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1
title_sort	fusaricidins, polymyxins and volatiles produced by <i>paenibacillus polymyxa</i> strains dsm 32871 and m1
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/66fc76f8632e45959828089ec4d5d7e0
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Fusaricidins, Polymyxins and Volatiles Produced by <i>Paenibacillus polymyxa</i> Strains DSM 32871 and M1

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