Replicate sequencing libraries are important for quantification of allelic imbalance

Allele-specific expression in diploid organisms can be quantified by RNA-seq and it is common practice to rely on a single library. Here, the authors show that the standard approach has variable error rate and present Qllelic as a tool to improve reproducibility of allele-specific RNA-seq analysis.

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Autores principales:	Asia Mendelevich, Svetlana Vinogradova, Saumya Gupta, Andrey A. Mironov, Shamil R. Sunyaev, Alexander A. Gimelbrant
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6708d342cd5e4c4980bf8799bffb923c
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spelling	oai:doaj.org-article:6708d342cd5e4c4980bf8799bffb923c2021-12-02T17:47:14ZReplicate sequencing libraries are important for quantification of allelic imbalance10.1038/s41467-021-23544-82041-1723https://doaj.org/article/6708d342cd5e4c4980bf8799bffb923c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23544-8https://doaj.org/toc/2041-1723Allele-specific expression in diploid organisms can be quantified by RNA-seq and it is common practice to rely on a single library. Here, the authors show that the standard approach has variable error rate and present Qllelic as a tool to improve reproducibility of allele-specific RNA-seq analysis.Asia MendelevichSvetlana VinogradovaSaumya GuptaAndrey A. MironovShamil R. SunyaevAlexander A. GimelbrantNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Asia Mendelevich Svetlana Vinogradova Saumya Gupta Andrey A. Mironov Shamil R. Sunyaev Alexander A. Gimelbrant Replicate sequencing libraries are important for quantification of allelic imbalance
description	Allele-specific expression in diploid organisms can be quantified by RNA-seq and it is common practice to rely on a single library. Here, the authors show that the standard approach has variable error rate and present Qllelic as a tool to improve reproducibility of allele-specific RNA-seq analysis.
format	article
author	Asia Mendelevich Svetlana Vinogradova Saumya Gupta Andrey A. Mironov Shamil R. Sunyaev Alexander A. Gimelbrant
author_facet	Asia Mendelevich Svetlana Vinogradova Saumya Gupta Andrey A. Mironov Shamil R. Sunyaev Alexander A. Gimelbrant
author_sort	Asia Mendelevich
title	Replicate sequencing libraries are important for quantification of allelic imbalance
title_short	Replicate sequencing libraries are important for quantification of allelic imbalance
title_full	Replicate sequencing libraries are important for quantification of allelic imbalance
title_fullStr	Replicate sequencing libraries are important for quantification of allelic imbalance
title_full_unstemmed	Replicate sequencing libraries are important for quantification of allelic imbalance
title_sort	replicate sequencing libraries are important for quantification of allelic imbalance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6708d342cd5e4c4980bf8799bffb923c
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Replicate sequencing libraries are important for quantification of allelic imbalance

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