Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats

BACKGROUND AND OBJECTIVE: Inflammation is a side effect of many diseases that could yield immune system disability. The recognized acute and chronic inflammations are the main factors for permanence of infection and long lasting in recovery of disease. Regarding to incomplete treatment of inflammati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Z Kiasalari, M Khalili, P Ahmadi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2011
Materias:	inflammation pain berberis vulgaris fruit rat Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/670c41910a3f4e548395aefec8a931f5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:670c41910a3f4e548395aefec8a931f5
record_format	dspace
spelling	oai:doaj.org-article:670c41910a3f4e548395aefec8a931f52021-11-10T08:56:18ZEffect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats1561-41072251-7170https://doaj.org/article/670c41910a3f4e548395aefec8a931f52011-02-01T00:00:00Zhttp://jbums.org/article-1-3715-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Inflammation is a side effect of many diseases that could yield immune system disability. The recognized acute and chronic inflammations are the main factors for permanence of infection and long lasting in recovery of disease. Regarding to incomplete treatment of inflammation by using chemical drugs and alkaloid compounds of barberry (berberis vulgaris) root bark such as Berberine that have strong anti-inflammatory property, in the present study, the anti-inflammatory effect of alcoholic extract of berberis vulgaris fruit using experimental methods is examined. METHODS: In this research as an experimental study, 86 male NMRI rats weighting 300-350 gr were chosen at random, and were divided into five groups: 1-control (only Evans blue), 2-sham (inflamed without treatment) 3, 4 and 5- treatment groups which received the alcoholic extract of barberry at doses of 75, 150 and 300 mg/kg intraperitoneally. Each of groups was subjected to three methods in order to measure the acute inflammation (formalin injection to paw, xylane to ear and intraperitoneally received acetic acid), and one group was examined with chronic inflammation (implantation of cotton in groin border of rats). Finally in a complementary assessment, the analgesic effect of the most effective dose of extract was evaluated using formalin test. FINDINGS: Effective doses of 150 and 300 mg/kg of berberis vulgaris fruit extract could change significantly the light absorption of inflamed foot, from 0.1±0.03 in control group to 0.15±0.02 and 0.16±0.01 respectively (p<0.05). In addition, 300 mg/kg dose of extract, increased the light absorption of peritoneal fluid significantly from 1.43±0.1 in control group relative to 0.7±0.09 in experimental group (p<0.05). Chronic inflammation has decreased significantly from 33.4±2.14 in control group relative to experimental groups (20.11±2.27 and 24.15±2.84, respectively) (p<0.01, p<0.05). Finally, the late phase of chronic pain has decreased significantly by dose of 300 mg/kg from 1.79± 0.18 (control) to 1.25± 0.1 (p<0.01).CONCLUSION: Our result shows that alcoholic extract of barberry in medium and maximum doses is able to decrease the formalin and acetic acid injection-induced inflammation in foot and peritoneum significantly and also can decrease an external body-induced chronic inflammation as well. In addition, the reduction of late phase of chronic pain in parallel with chronic inflammation suppression is considerable.Z KiasalariM KhaliliP AhmadiBabol University of Medical Sciencesarticleinflammationpainberberis vulgaris fruitratMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 1, Pp 28-35 (2011)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	inflammation pain berberis vulgaris fruit rat Medicine R Medicine (General) R5-920
spellingShingle	inflammation pain berberis vulgaris fruit rat Medicine R Medicine (General) R5-920 Z Kiasalari M Khalili P Ahmadi Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
description	BACKGROUND AND OBJECTIVE: Inflammation is a side effect of many diseases that could yield immune system disability. The recognized acute and chronic inflammations are the main factors for permanence of infection and long lasting in recovery of disease. Regarding to incomplete treatment of inflammation by using chemical drugs and alkaloid compounds of barberry (berberis vulgaris) root bark such as Berberine that have strong anti-inflammatory property, in the present study, the anti-inflammatory effect of alcoholic extract of berberis vulgaris fruit using experimental methods is examined. METHODS: In this research as an experimental study, 86 male NMRI rats weighting 300-350 gr were chosen at random, and were divided into five groups: 1-control (only Evans blue), 2-sham (inflamed without treatment) 3, 4 and 5- treatment groups which received the alcoholic extract of barberry at doses of 75, 150 and 300 mg/kg intraperitoneally. Each of groups was subjected to three methods in order to measure the acute inflammation (formalin injection to paw, xylane to ear and intraperitoneally received acetic acid), and one group was examined with chronic inflammation (implantation of cotton in groin border of rats). Finally in a complementary assessment, the analgesic effect of the most effective dose of extract was evaluated using formalin test. FINDINGS: Effective doses of 150 and 300 mg/kg of berberis vulgaris fruit extract could change significantly the light absorption of inflamed foot, from 0.1±0.03 in control group to 0.15±0.02 and 0.16±0.01 respectively (p<0.05). In addition, 300 mg/kg dose of extract, increased the light absorption of peritoneal fluid significantly from 1.43±0.1 in control group relative to 0.7±0.09 in experimental group (p<0.05). Chronic inflammation has decreased significantly from 33.4±2.14 in control group relative to experimental groups (20.11±2.27 and 24.15±2.84, respectively) (p<0.01, p<0.05). Finally, the late phase of chronic pain has decreased significantly by dose of 300 mg/kg from 1.79± 0.18 (control) to 1.25± 0.1 (p<0.01).CONCLUSION: Our result shows that alcoholic extract of barberry in medium and maximum doses is able to decrease the formalin and acetic acid injection-induced inflammation in foot and peritoneum significantly and also can decrease an external body-induced chronic inflammation as well. In addition, the reduction of late phase of chronic pain in parallel with chronic inflammation suppression is considerable.
format	article
author	Z Kiasalari M Khalili P Ahmadi
author_facet	Z Kiasalari M Khalili P Ahmadi
author_sort	Z Kiasalari
title	Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
title_short	Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
title_full	Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
title_fullStr	Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
title_full_unstemmed	Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats
title_sort	effect of alcoholic extract of berberis vulgaris fruit on acute and chronic inflammation in male rats
publisher	Babol University of Medical Sciences
publishDate	2011
url	https://doaj.org/article/670c41910a3f4e548395aefec8a931f5
work_keys_str_mv	AT zkiasalari effectofalcoholicextractofberberisvulgarisfruitonacuteandchronicinflammationinmalerats AT mkhalili effectofalcoholicextractofberberisvulgarisfruitonacuteandchronicinflammationinmalerats AT pahmadi effectofalcoholicextractofberberisvulgarisfruitonacuteandchronicinflammationinmalerats
_version_	1718440380662284288

Effect of Alcoholic Extract of Berberis Vulgaris Fruit on Acute and Chronic Inflammation in Male Rats

Ejemplares similares