Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.

Th17 cells and IL-17A play a role in the development and progression of allergic diseases. We analyzed the IL-17A levels in sputum supernatants (Ss), nasal wash (NW) and plasma (P) from Healthy Controls (HC) and children with Asthma/Rhinitis. We tested the expression of IL-17A, RORγ(t) and FOXP3 in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giusy Daniela Albano, Caterina Di Sano, Anna Bonanno, Loredana Riccobono, Rosalia Gagliardo, Pascal Chanez, Mark Gjomarkaj, Angela Marina Montalbano, Giulia Anzalone, Stefania La Grutta, Fabio Luigi Massimo Ricciardolo, Mirella Profita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67146814da9343c986b2b690651305ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:67146814da9343c986b2b690651305ed
record_format	dspace
spelling	oai:doaj.org-article:67146814da9343c986b2b690651305ed2021-11-18T07:50:49ZTh17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.1932-620310.1371/journal.pone.0058892https://doaj.org/article/67146814da9343c986b2b690651305ed2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573194/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Th17 cells and IL-17A play a role in the development and progression of allergic diseases. We analyzed the IL-17A levels in sputum supernatants (Ss), nasal wash (NW) and plasma (P) from Healthy Controls (HC) and children with Asthma/Rhinitis. We tested the expression of IL-17A, RORγ(t) and FOXP3 in peripheral blood T-lymphocytes from intermittent and mild-moderate asthma. The effect of Budesonide and Formoterol was tested "in vitro" on IL-17A, RORγ(t) and FOXP3 expression in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis patients, and on nasal and bronchial epithelial cells stimulated with NW and Ss from mild-moderate asthma/persistent rhinitis. Further, the effect of 12 weeks of treatment with Budesonide and Formoterol was tested "in vivo" in T-lymphocytes from mild-moderate asthma/persistent rhinitis patients. IL-17A was increased in Ss, NW and P from children with mild-moderate asthma compared with intermittent and HC. In cultured T-lymphocytes IL-17A and RORγ(t) expression were higher in mild-moderate asthma/persistent rhinitis than in mild-moderate asthma/intermittent rhinitis, while FOXP3 was reduced. Budesonide with Formoterol reduced IL-17A and RORγ(t), while increased FOXP3 in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis, and reduced the IL-8 release mediated by IL-17A present in NW and Ss from mild-moderate asthma/persistent rhinitis in nasal and bronchial epithelial cells. Finally, Budesonide with Formoterol reduced IL-17A levels in P and Ss, CD4(+)IL-17A(+)T-cells, in naïve children with mild-moderate asthma/persistent rhinitis after 12 weeks of treatment. Th17 mediated immunity may be involved in the airway disease of children with allergic asthma and allergic rhinitis. Budesonide with Formoterol might be a useful tool for its therapeutic control.Giusy Daniela AlbanoCaterina Di SanoAnna BonannoLoredana RiccobonoRosalia GagliardoPascal ChanezMark GjomarkajAngela Marina MontalbanoGiulia AnzaloneStefania La GruttaFabio Luigi Massimo RicciardoloMirella ProfitaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e58892 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giusy Daniela Albano Caterina Di Sano Anna Bonanno Loredana Riccobono Rosalia Gagliardo Pascal Chanez Mark Gjomarkaj Angela Marina Montalbano Giulia Anzalone Stefania La Grutta Fabio Luigi Massimo Ricciardolo Mirella Profita Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
description	Th17 cells and IL-17A play a role in the development and progression of allergic diseases. We analyzed the IL-17A levels in sputum supernatants (Ss), nasal wash (NW) and plasma (P) from Healthy Controls (HC) and children with Asthma/Rhinitis. We tested the expression of IL-17A, RORγ(t) and FOXP3 in peripheral blood T-lymphocytes from intermittent and mild-moderate asthma. The effect of Budesonide and Formoterol was tested "in vitro" on IL-17A, RORγ(t) and FOXP3 expression in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis patients, and on nasal and bronchial epithelial cells stimulated with NW and Ss from mild-moderate asthma/persistent rhinitis. Further, the effect of 12 weeks of treatment with Budesonide and Formoterol was tested "in vivo" in T-lymphocytes from mild-moderate asthma/persistent rhinitis patients. IL-17A was increased in Ss, NW and P from children with mild-moderate asthma compared with intermittent and HC. In cultured T-lymphocytes IL-17A and RORγ(t) expression were higher in mild-moderate asthma/persistent rhinitis than in mild-moderate asthma/intermittent rhinitis, while FOXP3 was reduced. Budesonide with Formoterol reduced IL-17A and RORγ(t), while increased FOXP3 in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis, and reduced the IL-8 release mediated by IL-17A present in NW and Ss from mild-moderate asthma/persistent rhinitis in nasal and bronchial epithelial cells. Finally, Budesonide with Formoterol reduced IL-17A levels in P and Ss, CD4(+)IL-17A(+)T-cells, in naïve children with mild-moderate asthma/persistent rhinitis after 12 weeks of treatment. Th17 mediated immunity may be involved in the airway disease of children with allergic asthma and allergic rhinitis. Budesonide with Formoterol might be a useful tool for its therapeutic control.
format	article
author	Giusy Daniela Albano Caterina Di Sano Anna Bonanno Loredana Riccobono Rosalia Gagliardo Pascal Chanez Mark Gjomarkaj Angela Marina Montalbano Giulia Anzalone Stefania La Grutta Fabio Luigi Massimo Ricciardolo Mirella Profita
author_facet	Giusy Daniela Albano Caterina Di Sano Anna Bonanno Loredana Riccobono Rosalia Gagliardo Pascal Chanez Mark Gjomarkaj Angela Marina Montalbano Giulia Anzalone Stefania La Grutta Fabio Luigi Massimo Ricciardolo Mirella Profita
author_sort	Giusy Daniela Albano
title	Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
title_short	Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
title_full	Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
title_fullStr	Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
title_full_unstemmed	Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
title_sort	th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/67146814da9343c986b2b690651305ed
work_keys_str_mv	AT giusydanielaalbano th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT caterinadisano th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT annabonanno th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT loredanariccobono th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT rosaliagagliardo th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT pascalchanez th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT markgjomarkaj th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT angelamarinamontalbano th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT giuliaanzalone th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT stefanialagrutta th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT fabioluigimassimoricciardolo th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach AT mirellaprofita th17immunityinchildrenwithallergicasthmaandrhinitisapharmacologicalapproach
_version_	1718422856505753600

Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.

Ejemplares similares