Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.

Lipopolysaccharide (LPS) is recognized by CD14 with Toll-like receptor 4 (TLR4), and initiates 2 major pathways of TLR4 signaling, the MyD88-dependent and TRIF-dependent signaling pathways. The MyD88-dependent pathway induces inflammatory responses such as the production of TNF-α, IL-6, and IL-12 vi...

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Autores principales:	Sachiko Watanabe, Yoshio Kumazawa, Joe Inoue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6721fa6ac81842ce943a8f7ef884b4d7
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spelling	oai:doaj.org-article:6721fa6ac81842ce943a8f7ef884b4d72021-11-18T07:51:04ZLiposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.1932-620310.1371/journal.pone.0060078https://doaj.org/article/6721fa6ac81842ce943a8f7ef884b4d72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23565187/?tool=EBIhttps://doaj.org/toc/1932-6203Lipopolysaccharide (LPS) is recognized by CD14 with Toll-like receptor 4 (TLR4), and initiates 2 major pathways of TLR4 signaling, the MyD88-dependent and TRIF-dependent signaling pathways. The MyD88-dependent pathway induces inflammatory responses such as the production of TNF-α, IL-6, and IL-12 via the activation of NFκB and MAPK. The TRIF-dependent pathway induces the production of type-I IFN, and RANTES via the activation of IRF-3 and NFκB, and is also important for the induction of adaptive immune responses. CD14 plays a critical role in initiating the TRIF-dependent signaling pathway response to LPS, to support the internalization of LPS via endocytosis. Here, we clearly demonstrate that intracellular delivery of LPS by LPS-formulated liposomes (LPS-liposomes) initiate only TRIF-dependent signaling via clathrin-mediated endocytosis, independent of CD14. In fact, LPS-liposomes do not induce the production of TNF-α and IL-6 but induce RANTES production in peritoneal macrophages. Additionally, LPS-liposomes could induce adaptive immune responses effectively in CD14-deficient mice. Collectively, our results strongly suggest that LPS-liposomes are useful as a TRIF-dependent signaling-based immune adjuvant without inducing unnecessary inflammation.Sachiko WatanabeYoshio KumazawaJoe InouePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60078 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sachiko Watanabe Yoshio Kumazawa Joe Inoue Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
description	Lipopolysaccharide (LPS) is recognized by CD14 with Toll-like receptor 4 (TLR4), and initiates 2 major pathways of TLR4 signaling, the MyD88-dependent and TRIF-dependent signaling pathways. The MyD88-dependent pathway induces inflammatory responses such as the production of TNF-α, IL-6, and IL-12 via the activation of NFκB and MAPK. The TRIF-dependent pathway induces the production of type-I IFN, and RANTES via the activation of IRF-3 and NFκB, and is also important for the induction of adaptive immune responses. CD14 plays a critical role in initiating the TRIF-dependent signaling pathway response to LPS, to support the internalization of LPS via endocytosis. Here, we clearly demonstrate that intracellular delivery of LPS by LPS-formulated liposomes (LPS-liposomes) initiate only TRIF-dependent signaling via clathrin-mediated endocytosis, independent of CD14. In fact, LPS-liposomes do not induce the production of TNF-α and IL-6 but induce RANTES production in peritoneal macrophages. Additionally, LPS-liposomes could induce adaptive immune responses effectively in CD14-deficient mice. Collectively, our results strongly suggest that LPS-liposomes are useful as a TRIF-dependent signaling-based immune adjuvant without inducing unnecessary inflammation.
format	article
author	Sachiko Watanabe Yoshio Kumazawa Joe Inoue
author_facet	Sachiko Watanabe Yoshio Kumazawa Joe Inoue
author_sort	Sachiko Watanabe
title	Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
title_short	Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
title_full	Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
title_fullStr	Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
title_full_unstemmed	Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
title_sort	liposomal lipopolysaccharide initiates trif-dependent signaling pathway independent of cd14.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6721fa6ac81842ce943a8f7ef884b4d7
work_keys_str_mv	AT sachikowatanabe liposomallipopolysaccharideinitiatestrifdependentsignalingpathwayindependentofcd14 AT yoshiokumazawa liposomallipopolysaccharideinitiatestrifdependentsignalingpathwayindependentofcd14 AT joeinoue liposomallipopolysaccharideinitiatestrifdependentsignalingpathwayindependentofcd14
_version_	1718422856693448704

Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.

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